Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	385,737	A→G	D169G (GAT→GGT)	tauA →	taurine ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	385,737	0	A	G	100.0%	159.4 / NA	45	D169G (GAT→GGT)	tauA	taurine ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/21);  total (24/21)

TTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTGGACGTCTGGGTGGTGCGCAAAGATTT  >  NC_000913/385616‑385869                                                                                                                          |                                                                                                                                     tttATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATg                                                                                                                     >  2:16271/1‑139 (MQ=255)              cccacTACAGCCTGCTGGCGGCACTGAGACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACcgg                                                                                                         <  1:665815/138‑1 (MQ=255)              cccacTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGTCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACcgg                                                                                                         >  2:665815/1‑138 (MQ=255)                        ccTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGcc                                                                                              >  1:988224/1‑139 (MQ=255)                        ccTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGcc                                                                                              >  2:211788/1‑139 (MQ=255)                        ccTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGcc                                                                                              >  1:571568/1‑139 (MQ=255)                            ctgGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTgg                                                                                          >  2:928842/1‑139 (MQ=255)                             tgGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGa                                                                                         >  2:992791/1‑139 (MQ=255)                                 ggcACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCGGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGaaaaa                                                                                     >  2:248955/1‑139 (MQ=255)                                   cACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGa                                                                                   >  1:472601/1‑139 (MQ=255)                                                gggCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTggg                                                                                                               <  2:344274/98‑1 (MQ=255)                                                gggCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTggg                                                                                                               >  1:344274/1‑98 (MQ=255)                                                  gCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGAc                                                                    <  2:571568/139‑1 (MQ=255)                                                      tAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGAt                                                                >  1:544441/1‑139 (MQ=255)                                                          cccGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTg                                                            >  2:181948/1‑139 (MQ=255)                                                              ggCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGgtg                                                                         >  2:964794/1‑122 (MQ=255)                                                              ggCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGgtg                                                                         <  1:964794/122‑1 (MQ=255)                                                                    tGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCgg                                                  <  1:1033561/139‑1 (MQ=255)                                                                     ggAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCggg                                                 <  1:992791/139‑1 (MQ=255)                                                                      gagaTTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAAc                                                         <  2:821840/130‑1 (MQ=255)                                                                      gagaTTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAAc                                                         >  1:821840/1‑130 (MQ=255)                                                                       agaTTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCa                                               <  2:988224/139‑1 (MQ=255)                                                                         aTTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGt                                             >  1:781678/1‑139 (MQ=255)                                                                             tgAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGgtg                                                                         <  1:583509/107‑1 (MQ=255)                                                                             tgAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGgtg                                                                         >  2:583509/1‑107 (MQ=255)                                                                              gAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGccc                                                                                             >  2:93476/1‑86 (MQ=255)                                                                              gAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGccc                                                                                             <  1:93476/86‑1 (MQ=255)                                                                                   tGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATgg                                                                                                                             <  2:684194/49‑1 (MQ=255)                                                                                   agCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATgg                                                                                                                             >  1:684194/2‑49 (MQ=38)                                                                                    gCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCa                                                                              <  1:83646/95‑1 (MQ=255)                                                                                    gCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCa                                                                              >  2:83646/1‑95 (MQ=255)                                                                                     cAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGGATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCATGGAACAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGTGGGGCGCGCCaa                                 <  1:118253/139‑1 (MQ=255)                                                                                          gcccgcGATTATCGCTGCCTGGCGGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTg                            <  1:248955/139‑1 (MQ=255)                                                                                          gcccgcGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTg                            <  2:472601/139‑1 (MQ=255)                                                                                           cccgcGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGAc                                                                    <  1:1098235/98‑1 (MQ=255)                                                                                           cccgcGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGAc                                                                    >  2:1098235/1‑98 (MQ=255)                                                                                            ccgcGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCt                             >  1:826755/1‑136 (MQ=255)                                                                                            ccgcGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCt                             <  2:826755/136‑1 (MQ=255)                                                                                             cgcgATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTGGAc                         >  1:3219/1‑139 (MQ=255)                                                                                                       gCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTGGACGTCTGggtgg               <  1:211788/139‑1 (MQ=255)                                                                                                               gCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTGGACGTCTGGGTGGTGCGCaaa       >  2:600052/1‑139 (MQ=255)                                                                                                                    ggggAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTGGACGTCTGGGTGGTGCGCAAAGAttt  <  2:781678/139‑1 (MQ=255)                                                                                                                    ggggAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTGGACGTCTGGGTGGTGCGCAAAGAttt  <  1:923938/139‑1 (MQ=255)                                                                                                                     gggAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGAc                                                                    >  2:810674/1‑72 (MQ=255)                                                                                                                     gggAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGAc                                                                    <  1:810674/72‑1 (MQ=255)                                                                                                                          |                                                                                                                                     TTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTGGACGTCTGGGTGGTGCGCAAAGATTT  >  NC_000913/385616‑385869

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑