| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 395,245 | G→A | A348V (GCG→GTG) | ampH ← | D‑alanyl‑D‑alanine‑ carboxypeptidase/endopeptidase, penicillin‑binding protein, weak beta‑lactamase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 395,245 | 0 | G | A | 98.5% | 209.2 / NA | 66 | A348V (GCG→GTG) | ampH | D‑alanyl‑D‑alanine‑ carboxypeptidase/endopeptidase, penicillin‑binding protein, weak beta‑lactamase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (31/34); minor base C (0/1); total (31/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACGCGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAACCGCCACCACCGCCTGTCTTCTGAATAATCCCCGGACGACCCTCTTTCGGGGCCATATATACCCAACCTAAACCAAGCGCATCCGCT > NC_000913/395110‑395376 | gTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACacgc < 1:801695/139‑1 (MQ=255) tttGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGcc > 1:438261/1‑139 (MQ=255) agTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATtcttct > 2:25471/1‑139 (MQ=255) aTCAGGACGCGGGAATAACCAACGCTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGat > 2:750574/1‑139 (MQ=255) agatcgcgGGGATAACCAACGGTTTATTTCTGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACGACCACAAACACTGCAATATTCTTCTGCGGGatcat < 2:248662/135‑1 (MQ=255) aGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGatcat > 2:809778/1‑139 (MQ=255) gACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGTCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGatcat < 2:678169/137‑1 (MQ=255) gACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGatcat > 1:678169/1‑137 (MQ=255) gACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCg > 1:5933/1‑139 (MQ=255) gACGCGGGGATAACCAACGGATTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCg > 2:1088616/1‑139 (MQ=255) cgcgGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACacgc < 1:611477/113‑1 (MQ=255) cgcgGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACacgc > 2:611477/1‑113 (MQ=255) gcgGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCa < 1:1032685/139‑1 (MQ=255) ggggATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCata < 2:438261/139‑1 (MQ=255) ggggATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCata < 1:1003261/139‑1 (MQ=255) ggATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCAtata < 2:751585/139‑1 (MQ=255) ggTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACCCGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAAc < 1:519791/139‑1 (MQ=255) tATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCata < 2:1105051/122‑1 (MQ=255) tATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCata > 1:1105051/1‑122 (MQ=255) ttCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAACCGccac > 1:1051607/1‑139 (MQ=255) gCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAACCGCCACCACCg > 2:281114/1‑139 (MQ=255) cTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAACCGCCACCACCGc < 1:809778/139‑1 (MQ=255) tAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAACCGCCACCACCGCCt < 2:5933/139‑1 (MQ=255) gCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAACCGCCACCACCGCCTGt < 1:1088616/139‑1 (MQ=255) gTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCata > 2:257629/1‑105 (MQ=255) gTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCata < 1:257629/105‑1 (MQ=255) aCCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAACCGCCACCACCGCCTGTCTTCTGaa < 1:281114/139‑1 (MQ=255) tGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACTAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAACCGCCACCACCGCCt < 2:797191/119‑1 (MQ=255) tGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAACCGCCACCACCGCCt > 1:797191/1‑119 (MQ=255) gATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAACCGCCACCACCGCCTGTCTTCTGAATAATCCCCGga < 1:25471/139‑1 (MQ=255) aTGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCata < 2:870656/90‑1 (MQ=255) aTGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCata > 1:870656/1‑90 (MQ=255) ccATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAACCGCCACCACCGc < 1:228778/112‑1 (MQ=255) ccATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAACCGCCACCACCGc < 1:422460/112‑1 (MQ=255) ccATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAACCGCCACCACCGc > 2:422460/1‑112 (MQ=255) ccATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAACCGCCACCACCGc > 2:228778/1‑112 (MQ=255) cATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCAtata < 2:888286/88‑1 (MQ=255) cATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCAtata > 1:888286/1‑88 (MQ=255) atTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAACCGCCACCACCGCCTGTCTTCTGAATAATCCCCGGACGACCCTCTTTCggg < 1:401087/139‑1 (MQ=255) tttttAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAACCGCCACCACCGCCTGTCTTCTGAATAATCCCCGGACGACCCTCTTTCgggg < 1:750574/139‑1 (MQ=255) tttttAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAACCGCCACCACCGCCTGTCTTCTGAATAATCCCCGGACGACCCTCTTTCgggg < 2:1051607/139‑1 (MQ=255) ttttAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAACCGCCACCACCGCCTGTCTTCTGAATAATCCCCGGACGACCCTCTTTCGGGGc < 2:772464/139‑1 (MQ=255) ttttAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAACCGCCACCACCGCCTGTCTTCTGAATAATCCCCGGACGACCCTCTTTCGGGGc < 2:247855/139‑1 (MQ=255) aGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAACCGCCACCACCGCCTGTCTTCTGAATAATCCCCGGACGACCCTCTTTCgggg > 2:597823/1‑132 (MQ=255) aGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAACCGCCACCACCGCCTGTCTTCTGAATAATCCCCGGACGACCCTCTTTCgggg < 1:597823/132‑1 (MQ=255) cgcgTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGatcat > 1:71008/1‑59 (MQ=255) cgcgTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGatcat < 2:71008/59‑1 (MQ=255) cgcgTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGat > 1:487194/1‑56 (MQ=255) cgcgTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGat < 2:487194/56‑1 (MQ=255) cgcgTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAACCGCCACCACCGCCTGTCTTCTGAATAATCCCCGGACGACCCTCTTTCGGGGCCAtatata > 1:57879/1‑139 (MQ=255) gcgTCAACGGCGAACGAGTGACCACCACAAACacgc < 2:723624/36‑1 (MQ=255) gcgTCAACGGCGAACGAGTGACCACCACAAACACGCCAata < 2:333062/41‑1 (MQ=255) gcgTCAACGGCGAACGAGTGACCACCACAAACACGCCAata > 1:333062/1‑41 (MQ=255) gcgTCAACAGCGAACGAGTGACCACCACAAACacgc > 1:723624/1‑36 (MQ=255) cgTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGat < 2:394464/54‑1 (MQ=255) cgTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGat > 1:394464/1‑54 (MQ=255) cgTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGa < 2:526714/53‑1 (MQ=255) cgTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGa > 1:526714/1‑53 (MQ=255) cgTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAACCGc < 2:310086/80‑1 (MQ=255) cgTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAACCGc > 1:310086/1‑80 (MQ=255) cgTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAACCGCCACCACCGc < 1:41762/89‑1 (MQ=255) cgTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAACCGCCACCACCGc > 2:41762/1‑89 (MQ=255) cgTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAACCGCCACCACCGCCTGTCTTCTGAATAATCCCCGGACGACCCTCTTTCGGGGCCATATATAcc > 1:481702/1‑139 (MQ=255) aaCGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAACCGCCACCACCGCCTGTCTTCTGAATAATCCCCGGACGACCCTCTTTCGGGGCCATATATACCCAACCTAAAcc > 2:414824/1‑139 (MQ=255) accaccACAAACACGCCAATATTCTTCTGCGGGATCATCGCCTTATAGGTAATGAAACCGCCACCACCGCCTGTCTTCTGAATAATCCCCGGACGACCCTCTTTCGGGGc > 1:771570/1‑110 (MQ=255) accaccACAAACACGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAACCGccac > 2:771696/1‑64 (MQ=255) accaccACAAACACGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAACCGccac < 1:771696/64‑1 (MQ=255) accaccACAAACACGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAACCGCCACCACCGCCTGTCTTCTGAATAATCCCCGGACGACCCTCTTTCGGGGc < 2:771570/110‑1 (MQ=255) caccacAAACACGCCAATATTCTTCTGCGGGATCATCGCCata > 2:6061/1‑43 (MQ=255) caccacAAACACGCCAATATTCTTCTGCGGGATCATCGCCata < 1:6061/43‑1 (MQ=255) cacaAACACGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAACCGCCACCACCGCCTGTCTTCTGAATAATCCCCGGACGACCCTCTTTCGGGGCCATATATACCCAACCTAAACCAAGCGCATCCGCt > 1:165209/1‑139 (MQ=255) | GTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACGCGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAACCGCCACCACCGCCTGTCTTCTGAATAATCCCCGGACGACCCTCTTTCGGGGCCATATATACCCAACCTAAACCAAGCGCATCCGCT > NC_000913/395110‑395376 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |