Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	400,720	C→T	A68A (GCG→GCA)	ddlA ←	D‑alanine‑D‑alanine ligase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	400,720	0	C	T	100.0%	90.6 / NA	29	A68A (GCG→GCA)	ddlA	D‑alanine‑D‑alanine ligase A
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/17);  total (12/17)

GCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTACCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAACGCAATATGGGCAGGATCGTCTGCATTTAGCAGATAATTGCTGGCATCGCTGACGTGCCATTGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACA  >  NC_000913/400596‑400843                                                                                                                             |                                                                                                                            gCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTACCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAATGCAATATGGGCAgg                                                                                                               <  1:1062647/139‑1 (MQ=255) gCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTACCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAATGCAATATGGGCAgg                                                                                                               <  1:293987/139‑1 (MQ=255) gCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTACCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAATGCAATATGGGCAgg                                                                                                               <  1:963494/139‑1 (MQ=255)  cGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTACCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAATGCAATATGGGCAGGa                                                                                                              >  2:300361/1‑139 (MQ=255)      ccGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTACCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAATGCAATATGGGCAGGATCGt                                                                                                          <  2:763682/139‑1 (MQ=255)      ccGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTACCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAATGCAATATGGGCAGGATCGt                                                                                                          <  1:286134/139‑1 (MQ=255)           gACAATCGGGAAAATGACATCCACCGTCAGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTACCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAATGCAATATGGGCAGGATCGTCTGCa                                                                                                     >  2:6107/1‑139 (MQ=255)                tCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTACCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAATGCAATATGGGCAGGATCGTCTGCa                                                                                                     >  2:78150/1‑134 (MQ=255)                tCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTACCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAATGCAATATGGGCAGGATCGTCTGCa                                                                                                     <  1:78150/134‑1 (MQ=255)                tCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTACCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAATGCAATATGGGCAGGATCGTCTGCATTTAg                                                                                                <  1:139381/139‑1 (MQ=255)                tCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTACCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAATGCAATATGGGCAGGATCGTCTGCATTTAg                                                                                                >  2:112957/1‑139 (MQ=255)                          gACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTACCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAATGCAATATGGGCAGGATCGTCTGCATTTAGCAGATAATTg                                                                                      >  1:839988/1‑139 (MQ=255)                             aTCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTACCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAATGCAATATGGGCAGGATCGTCTGCATTTAGCAGATAATTGCTg                                                                                   <  2:152516/139‑1 (MQ=255)                                cACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTACCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAATGCAATATGGGCAGGATCGTCTGCATTTAGCAGATAATTGCTGGCa                                                                                <  1:1082842/139‑1 (MQ=255)                                  ccGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTACCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAATGCAATATGGGCAGGATCGTCTGCATTTAGCAGATAATTGCTGGCATc                                                                              >  2:529329/1‑139 (MQ=255)                                   cGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTACCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCgcaatgcaat                                                                                                                        <  1:912572/96‑1 (MQ=255)                                   cGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTACCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCgcaatgcaat                                                                                                                        >  2:912572/1‑96 (MQ=255)                                     tCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTACCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAATGCAATATGGGCAGGATCGTCTGCATTTAGCAGATAATTGCTGGCATCGCt                                                                           >  2:178379/1‑139 (MQ=255)                                                   ccGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTACCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAATGCAATATGGGCAGGATCGTCTGCATTTAGCAGATAATTGCTGGCATCGCTGACGTGCCATTGcc                                                             <  2:345408/139‑1 (MQ=255)                                                    cGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTACCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAATGCAATATGGGCAGGATCGTCTGCATTTAGCAGATAATTGCTGGCATCGCTGACGTGCCATTGccc                                                            <  1:6107/139‑1 (MQ=255)                                                    cGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTACCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAATGCAATATGGGCAGGATCGTCTGCATTTAGCAGATAATTGCTGGCATCGCTGACGTGCCATTGccc                                                            <  2:954088/139‑1 (MQ=255)                                                                          aTGCTCATGTTTACCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAATGCAATATGGGCAGGATCGTCTGCATTTAGCAGATAATTGCTGGCATCGCTGACGTGCCATTGCCCTTGTTTATCAATGCCCagcagc                                      >  1:19567/1‑139 (MQ=255)                                                                                          ggCACCTGCGCAAGGCTGGTCGCCGAAGGGCgcaatgcaat                                                                                                                        >  1:392949/1‑41 (MQ=255)                                                                                          ggCACCTGCGCAAGGCTGGTCGCCGAAGGGCgcaatgcaat                                                                                                                        <  2:392949/41‑1 (MQ=255)                                                                                          ggCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAATGCAATATGGGCa                                                                                                                 <  1:68410/48‑1 (MQ=255)                                                                                          ggCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAATGCAATATGGGCa                                                                                                                 >  2:68410/1‑48 (MQ=255)                                                                                             aCCTGCGCAAGGCTGGTCGCCGAAGGGCGCAATGCAATATGGGCAGGATCGTCTGCATTTAGCAGATAATTGCTGGCATCGCTGACGTGCCATTGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAg                          <  2:96760/132‑1 (MQ=255)                                                                                             aCCTGCGCAAGGCTGGTCGCCGAAGGGCGCAATGCAATATGGGCAGGATCGTCTGCATTTAGCAGATAATTGCTGGCATCGCTGACGTGCCATTGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAg                          >  1:96760/1‑132 (MQ=255)                                                                                             aCCTGCGCAAGGCTGGTCGCCGAAGGGCGCAATGCAATATGGGCAGGATCGTCTGCATTTAGCAGATAATTGCTGGCATCGCTGACGTGCCATTGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACttt                   <  1:178379/139‑1 (MQ=255)                                                                                                            gTCGCCGAAGGGCGCAATGCAATATGGGCAGGATCGTCTGCATTTAGCAGATAATTGCTGGCATCGCTGACGTGCCATTGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAg                          <  2:680461/117‑1 (MQ=255)                                                                                                            gTCGCCGAAGGGCGCAATGCAATATGGGCAGGATCGTCTGCATTTAGCAGATAATTGCTGGCATCGCTGACGTGCCATTGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAg                          >  1:680461/1‑117 (MQ=255)                                                                                                            gTCGCCGAAGGGCGCAATGCAATATGGGCAGGATCGTCTGCATTTAGCAGATAATTGCTGGCATCGCTGACGTGCCATTGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGa    >  2:316847/1‑139 (MQ=255)                                                                                                              cGCCGAAGGGCGCAATGCAATATGGGCAGGATCGTCTGCATTTAGCAGATAATTGCTGGCATCGCTGACGTGCCATTGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACa  <  1:419788/139‑1 (MQ=255)                                                                                                                             |                                                                                                                            GCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTACCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAACGCAATATGGGCAGGATCGTCTGCATTTAGCAGATAATTGCTGGCATCGCTGACGTGCCATTGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACA  >  NC_000913/400596‑400843

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑