Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	49,765	C→T	intergenic (+134/‑58)	kefC → / → folA	potassium:proton antiporter/dihydrofolate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	49,765	0	C	T	100.0%	173.9 / NA	54	intergenic (+134/‑58)	kefC/folA	potassium:proton antiporter/dihydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/28);  total (26/28)

AGTGACGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTG  >  NC_000913/49637‑49894                                                                                                                                 |                                                                                                                                  agTGACGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGtt                                                                                                                         >  1:1109988/1‑139 (MQ=255) agTGACGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGtt                                                                                                                         >  2:958791/1‑139 (MQ=255)  gTGACGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGttt                                                                                                                        >  2:347932/1‑139 (MQ=255)     aCGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACg                                                                                                                     >  2:664989/1‑139 (MQ=255)      cGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGc                                                                                                                    <  2:1109988/139‑1 (MQ=255)      cGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGc                                                                                                                    <  2:377001/139‑1 (MQ=255)       gTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATTCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCAAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCt                                                                                                                   <  1:818285/139‑1 (MQ=255)       gTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCt                                                                                                                   <  1:87946/139‑1 (MQ=255)       gTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCt                                                                                                                   <  1:664989/139‑1 (MQ=255)                    tACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAGCATCATCCTCGCACCAGTTGGCGACGGttt                                                                                                                        <  2:410072/121‑1 (MQ=255)                    tACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGttt                                                                                                                        >  1:410072/1‑121 (MQ=255)                       ccgcattacaatgtttttgtttCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGa                                                                                                   >  1:375625/12‑139 (MQ=255)                          tAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACaa                                                                                                >  2:19330/1‑139 (MQ=255)                          tAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACaa                                                                                                >  1:91126/1‑139 (MQ=255)                            aCTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGc                                                                                                                    <  2:457019/117‑1 (MQ=255)                            aCTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGc                                                                                                                    >  1:457019/1‑117 (MQ=255)                              tgtttgttTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCt                                                                                                                   <  2:761754/116‑1 (MQ=255)                              tgtttgttTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCt                                                                                                                   >  1:761754/1‑116 (MQ=255)                                tttgtttttgttTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAAtttttt                                                                                          >  1:192256/1‑139 (MQ=255)                                        tgtttCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCttt                                                                                                                 <  2:819274/108‑1 (MQ=255)                                        tgtttCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCttt                                                                                                                 >  1:819274/1‑108 (MQ=255)                                         gtttCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGa                                                                                 <  2:192256/139‑1 (MQ=255)                                                    aTGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTAc                                                                                                                      <  2:599524/91‑1 (MQ=255)                                                    aTGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTAc                                                                                                                      >  1:599524/1‑91 (MQ=255)                                                    aTGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCttt                                                                                                                 <  2:615398/96‑1 (MQ=255)                                                    aTGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCttt                                                                                                                 >  1:615398/1‑96 (MQ=255)                                                     tGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGAt                                                                     <  1:347932/139‑1 (MQ=255)                                                       cggcgAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTAc                                                                                                                      >  1:915024/1‑88 (MQ=255)                                                       cggcgAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTAc                                                                                                                      <  2:915024/88‑1 (MQ=255)                                                         gcgAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGt                                                                 >  1:1033788/1‑139 (MQ=255)                                                          cgAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTc                                                                <  1:221092/139‑1 (MQ=255)                                                                      gagagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGt                                                    <  1:958791/139‑1 (MQ=255)                                                                       agagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAAtctc                                                                           >  2:381492/1‑115 (MQ=255)                                                                       agagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAAtctc                                                                           <  1:381492/115‑1 (MQ=255)                                                                        gagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCt                                                                                                                   <  2:58802/74‑1 (MQ=255)                                                                        gagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCt                                                                                                                   >  1:58802/1‑74 (MQ=255)                                                                        gagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGt                                                    >  1:789313/1‑137 (MQ=255)                                                                        gagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGt                                                    <  2:789313/137‑1 (MQ=255)                                                                          gCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGc                                                <  2:912419/139‑1 (MQ=255)                                                                                 cTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGAt                                         <  2:375625/139‑1 (MQ=255)                                                                                         cagAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGAt                                                                     >  1:170653/1‑103 (MQ=255)                                                                                         cagAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGAt                                                                     <  2:170653/103‑1 (MQ=255)                                                                                                        tCCTCAACATCATCCTCGCACCAGTTGGCGACGGttt                                                                                                                        >  2:962170/1‑37 (MQ=255)                                                                                                        tCCTCAACATCATCCTCGCACCAGTTGGCGACGGttt                                                                                                                        <  1:962170/37‑1 (MQ=255)                                                                                                                  catcCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTgg        >  1:8797/1‑139 (MQ=255)                                                                                                                    tcCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCg           >  2:607366/1‑134 (MQ=255)                                                                                                                    tcCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCg           <  1:607366/134‑1 (MQ=255)                                                                                                                       tCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAAcct   >  1:525991/1‑139 (MQ=255)                                                                                                                       tCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAAcct   <  2:8797/139‑1 (MQ=255)                                                                                                                        cGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAAcctg  <  1:290492/139‑1 (MQ=255)                                                                                                                          cACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGaaa                                                                               <  1:49969/60‑1 (MQ=255)                                                                                                                          cACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGaaa                                                                               >  2:49969/1‑60 (MQ=255)                                                                                                                          cACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGa                                                                                 <  1:28386/58‑1 (MQ=255)                                                                                                                          cACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGa                                                                                 >  2:28386/1‑58 (MQ=255)                                                                                                                                 |                                                                                                                                  AGTGACGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTG  >  NC_000913/49637‑49894

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑