Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	444,641	C→T	intergenic (‑126/‑42)	panE ← / → yajQ	2‑dehydropantoate reductase, NADPH‑specific/phage Phi6 host factor, ATP/GTP binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	444,641	0	C	T	100.0%	151.6 / NA	48	intergenic (‑126/‑42)	panE/yajQ	2‑dehydropantoate reductase, NADPH‑specific/phage Phi6 host factor, ATP/GTP binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/23);  total (25/23)

TGTTCCTCCTCACCCGCAACCACTCCGGGTGTTCAATAAGGCTATCCCTTAATTGTGCATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGCGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGATCTTCAGGAAGCACGTAACGCGGTCGATAACGCGAGCCGCGAAGTGGAGTCCCGTTTT  >  NC_000913/444516‑444772                                                                                                                              |                                                                                                                                    tGTTCCTCCTCACCCGCAACCACTCCGGGTGTTCAATAAGGCTATCCCTTAATTGTGCATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTggg                                                                                                                                  <  2:520653/129‑1 (MQ=255) tGTTCCTCCTCACCCGCAACCACTCCGGGTGTTCAATAAGGCTATCCCTTAATTGTGCATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTggg                                                                                                                                  >  1:520653/1‑129 (MQ=255)              ccGCAACCACTCCGGGTGTTCAATAAGGCTATCCCTTAATTGTGCATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATg                                                                                                           <  1:924046/139‑1 (MQ=255)              ccGCAACCACTCCGGGTGTTCAATAAGGCTATCCCTTAATTGGGCATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTTGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATg                                                                                                           <  2:1081477/139‑1 (MQ=255)                  aaCCACTCCGGGTGTTCAATAAGGCTATCCCTTAATTGTGCATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAgg                                                                                                       <  1:440365/139‑1 (MQ=255)                            ggTGTTCAATAAGGCTATCCCTTAATTGTGCATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAggg                                                                                                      >  2:458877/1‑130 (MQ=255)                            ggTGTTCAATAAGGCTATCCCTTAATTGTGCATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAggg                                                                                                      <  1:458877/130‑1 (MQ=255)                                     tAAGGCTATCCCTTAATTGTGCATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATc                                                                                    <  2:328001/139‑1 (MQ=255)                                             ccccTTAATTGTGCATGCTGTTGCGACTATGCACAATTAAGCGATACGTCCTGGTGCAGGACTGTCGGTTATTAAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAGGATGCCATCTTTCGata                                                                            >  1:682710/2‑139 (MQ=255)                                                       gtgCATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCa                                                                                                                 <  2:319963/92‑1 (MQ=255)                                                       gtgCATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCa                                                                                                                 >  1:319963/1‑92 (MQ=255)                                                          cATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGt                                                               >  1:136894/1‑139 (MQ=255)                                                               tGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGgag                                                                                                    <  2:515577/97‑1 (MQ=255)                                                               tGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGgag                                                                                                    >  1:515577/1‑97 (MQ=255)                                                               tGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGt                                                               <  2:810345/134‑1 (MQ=255)                                                               tGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGt                                                               >  1:810345/1‑134 (MQ=255)                                                                  tGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGt                                                               >  1:205989/1‑131 (MQ=255)                                                                  tGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGt                                                               <  2:205989/131‑1 (MQ=255)                                                                  tGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGATCTTc                                                       <  1:311508/139‑1 (MQ=255)                                                                      aCTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGATCTTCAGGa                                                   >  2:360185/1‑139 (MQ=255)                                                                      aCTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGATCTTCAGGa                                                   <  2:205146/139‑1 (MQ=255)                                                                         aTGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGc                                                                                        >  1:566854/1‑99 (MQ=255)                                                                         aTGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGc                                                                                        <  2:566854/99‑1 (MQ=255)                                                                         aTGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGATCTTCAGGAAGc                                                >  2:579656/1‑139 (MQ=255)                                                                                 ttAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGATCTTCAGGAAGCACGTAAcg                                        >  2:1109080/1‑139 (MQ=255)                                                                                         tACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGCGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGATCTTCACGAAGCACGTAACGCGGTCGAt                                >  2:646442/1‑139 (MQ=255)                                                                                         tACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGtctc                                                                     >  1:1060881/1‑102 (MQ=255)                                                                                         tACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGtctc                                                                     <  2:1060881/102‑1 (MQ=255)                                                                                         tACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGATCTTCAGGAAGCACGTa                                           >  2:743526/1‑128 (MQ=255)                                                                                         tACGTCCTGGTGCAGGACTGTCGGTTATTGAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCTTCTTTTGATATTGTCTCTGAAGTTGATCTTCAGGAAGCACGTa                                           <  1:743526/128‑1 (MQ=255)                                                                                           cGTCCTGGTGCAGGACTGTCGGTTATTTACCTTAGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGACATCCTTCGATATTGTCTCTGAAGTTGATCTTCAGGAAGCACGTATCGCGCTCGTTaa                              >  2:954040/1‑139 (MQ=255)                                                                                           cGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGatat                                                                           >  2:872600/1‑94 (MQ=255)                                                                                           cGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGatat                                                                           <  1:872600/94‑1 (MQ=255)                                                                                           cGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGa                                                            <  2:222526/109‑1 (MQ=255)                                                                                           cGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGa                                                            >  1:222526/1‑109 (MQ=255)                                                                                           cGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGATCTTCAGGAAGCACGTAACGCGGTCGATaa                              >  2:240512/1‑139 (MQ=255)                                                                                                 ggTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGATCTTCAGGAAGCACGTAACGCGGTCGATAACGCGAg                        >  2:1109647/1‑139 (MQ=255)                                                                                                           tGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGATCTTCAGGAAGCACGTAACGCGGTCGATAACGCGAGCCGCGAAGTg              <  1:360185/139‑1 (MQ=255)                                                                                                             tCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGcc                                                                                       >  1:892439/1‑64 (MQ=255)                                                                                                             tCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGcc                                                                                       <  2:892439/64‑1 (MQ=255)                                                                                                             tCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGa                                                            >  1:1005555/1‑91 (MQ=255)                                                                                                             tCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGa                                                            <  2:1005555/91‑1 (MQ=255)                                                                                                               ggTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGATCTTCAGGAAGCACGTAACGCGGTCGATAACGCGAGCCGCGAAGTGGAGt          <  1:1109080/139‑1 (MQ=255)                                                                                                                  tATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGat                                                                             <  1:123514/69‑1 (MQ=255)                                                                                                                  tATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGat                                                                             >  2:123514/1‑69 (MQ=255)                                                                                                                  tATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGtctc                                                                     <  2:207626/77‑1 (MQ=255)                                                                                                                  tATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGtctc                                                                     >  1:207626/1‑77 (MQ=255)                                                                                                                       aaCTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGATCTTCAGGAAGCACGTAACGCGGTCGATAACGCGAGCCGCGAAGTGGAGTCCCGtttt  >  2:399990/1‑139 (MQ=255)                                                                                                                              |                                                                                                                                    TGTTCCTCCTCACCCGCAACCACTCCGGGTGTTCAATAAGGCTATCCCTTAATTGTGCATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGCGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGATCTTCAGGAAGCACGTAACGCGGTCGATAACGCGAGCCGCGAAGTGGAGTCCCGTTTT  >  NC_000913/444516‑444772

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑