Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	470,643	G→A	*591* (TAG→TAA)  S3N (AGT→AAT)	mdlA → mdlB →	putative multidrug ABC transporter ATPase putative multidrug ABC transporter ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	470,643	0	G	A	100.0%	185.2 / NA	57	*591* (TAG→TAA)  S3N (AGT→AAT)	mdlA mdlB	putative multidrug ABC transporter ATPase putative multidrug ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/30);  total (27/30)

GTGGCAATCATGATGTGCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAGTTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGATTGCGGTCCTGATGATGTGGGTTGCGGCGGCGGCAGAAGTCAGTGGGCCGCTGCTTATCAG  >  NC_000913/470522‑470775                                                                                                                          |                                                                                                                                     gTGGCAATCATGATGTGCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGc                                                                                                                     <  2:738595/139‑1 (MQ=255)    gCAATCATGATGTGCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGa                                                                                                                  <  2:659671/139‑1 (MQ=255)            gatgTGCTGACACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAg                                                                                                          <  1:718296/139‑1 (MQ=255)              tgtgCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAgcg                                                                                                        >  2:975024/1‑139 (MQ=255)                  cTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTg                                                                                                    >  1:965806/1‑139 (MQ=255)                  cTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTg                                                                                                    >  2:588924/1‑139 (MQ=255)                           caaAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGACCGACTCTCAAGCGCCTGTTAGCGTAc                                                                                           >  1:948010/1‑139 (MQ=255)                              aGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAgc                                                                                                         <  1:227426/122‑1 (MQ=255)                              aGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAgc                                                                                                         >  2:227426/1‑122 (MQ=255)                                   cTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGcc                                                                                   <  1:975024/139‑1 (MQ=255)                                    tGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCg                                                                                  <  2:33261/139‑1 (MQ=255)                                      gTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGACCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTg                                                                                <  2:948010/139‑1 (MQ=255)                                          cgcgATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGAAGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGt                                                                            >  2:785992/1‑139 (MQ=255)                                           gcgATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTa                                                                           <  2:1070046/139‑1 (MQ=255)                                            cgATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTaa                                                                          >  1:873805/1‑139 (MQ=255)                                                 tGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGc                                                                     >  2:1021524/1‑139 (MQ=255)                                                 tGTATCGCTATCAACAACTGCAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGc                                                                     >  2:220430/1‑139 (MQ=255)                                                      cGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGcc                                                                                                                              >  2:372049/1‑77 (MQ=255)                                                      cGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGcc                                                                                                                              <  1:372049/77‑1 (MQ=255)                                                          aTCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGATTg                                                            <  2:1050/139‑1 (MQ=255)                                                          aTCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGATTg                                                            <  1:1021524/139‑1 (MQ=255)                                                           tCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAgcgc                                                                                                       >  1:891874/1‑95 (MQ=255)                                                           tCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAgcgc                                                                                                       <  2:891874/95‑1 (MQ=255)                                                           tCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTaaa                                                                         >  2:619383/1‑125 (MQ=255)                                                           tCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTaaa                                                                         <  1:619383/125‑1 (MQ=255)                                                           tCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGATTGc                                                           >  2:347133/1‑139 (MQ=255)                                                           tCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGATTGc                                                           >  2:778677/1‑139 (MQ=255)                                                           tCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGATTGc                                                           >  2:272238/1‑139 (MQ=255)                                                             aacaacTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGAc                                                                                                                 >  2:30076/1‑83 (MQ=255)                                                             aacaacTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGAc                                                                                                                 <  1:30076/83‑1 (MQ=255)                                                             aacaacTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGATTGCgg                                                         <  1:588924/139‑1 (MQ=255)                                                             aacaacTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGATTGCgg                                                         <  1:778677/139‑1 (MQ=255)                                                                  cTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGATTGCGGTCCtg                                                    <  2:965806/139‑1 (MQ=255)                                                                   tGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGa                                                                                                                  >  2:277822/1‑76 (MQ=255)                                                                   tGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGa                                                                                                                  <  1:277822/76‑1 (MQ=255)                                                                   tGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGATTGCGATCCtga                                                   >  1:507840/1‑139 (MQ=255)                                                                     gAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCGAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGATTGCgg                                                         >  2:1105553/1‑131 (MQ=255)                                                                     gAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCGAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGATTGCgg                                                         <  1:1105553/131‑1 (MQ=255)                                                                     gAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGATTGCGGTCCtgatg                                                 >  2:427147/1‑139 (MQ=255)                                                                       ggcggcGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGATTGCGGTCCTgatgat                                               >  2:521639/1‑139 (MQ=255)                                                                               tCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGcc                                                                                   <  2:11736/95‑1 (MQ=255)                                                                               tCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGcc                                                                                   >  1:11736/1‑95 (MQ=255)                                                                                     acgCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGATTGCGATCCTGATGATGTGGGTTgcggcgg                                 <  2:507840/139‑1 (MQ=255)                                                                                        cTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGcc                                                                                   <  2:756054/86‑1 (MQ=255)                                                                                        cTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGcc                                                                                   >  1:756054/1‑86 (MQ=255)                                                                                                aaTCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGc                                                                                                                     >  1:1096157/1‑44 (MQ=255)                                                                                                aaTCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGc                                                                                                                     <  2:1096157/44‑1 (MQ=255)                                                                                                       aggaggCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGATTGCGGTCCTGATGATGTGGGTTGCGGCGGCGGCAGAAGTCAGTGGGc               <  1:521639/139‑1 (MQ=255)                                                                                                          aggCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGATTGCGGTCCTGATGATGTGGGTTGCGGCGGCGGCAGAAGTCAGTGGGCCgc            <  1:785992/139‑1 (MQ=255)                                                                                                          aggCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGATTGCGGTCCTGATGATGTGGGTTGCGGCGGCGGCAGAAGTCAGTGGGCCgc            <  1:745705/139‑1 (MQ=255)                                                                                                            gCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGATTGCGGTCCTGATGATGTGGGTTGCGGCGGCGGCAGAAGTCAGTGGGCCgctg          <  1:823531/139‑1 (MQ=255)                                                                                                              cGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGATTGCGGTCCTGATGATGTGGGTTGCGGCGGCGGCAGAAGTCAGTGGGCCgctgct        >  2:790391/1‑139 (MQ=255)                                                                                                                  gATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCg                                                                      <  1:877199/73‑1 (MQ=255)                                                                                                                  gATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCg                                                                      >  2:877199/1‑73 (MQ=255)                                                                                                                  gATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGATTGCGGTCCTGATGATGTGGGTTGCGGCGGCGGCAGAAGTCAGTGGGCCGCTGCTTATc    <  1:347133/139‑1 (MQ=255)                                                                                                                    tGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGATTGCGGTCCTGATGATGTGGGTTGCGGCGGCGGCAGAAGTCAGTGGGCCGCTGCTTATCAg  <  1:790391/139‑1 (MQ=255)                                                                                                                    tGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGATTGCGGTCCTGATGATGTGGGTTGCGGCGGCGGCAGAAGTCAGTGGGCCGCTGCTTATCAg  >  2:1042648/1‑139 (MQ=255)                                                                                                                          |                                                                                                                                     GTGGCAATCATGATGTGCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAGTTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGATTGCGGTCCTGATGATGTGGGTTGCGGCGGCGGCAGAAGTCAGTGGGCCGCTGCTTATCAG  >  NC_000913/470522‑470775

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑