Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,685,542 (C)6→5 coding (1047/1404 nt) fumC ← fumarate hydratase (fumarase C),aerobic Class II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,685,5370C.100.0% 148.8 / NA 36coding (1052/1404 nt)fumCfumarate hydratase (fumarase C),aerobic Class II
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (15/21);  total (15/21)

CTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTC  >  NC_000913/1685401‑1685667
                                                                                                                                        |                                                                                                                                  
cTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGccc                                                                                                                                  <  1:71842/139‑1 (MQ=255)
       ttCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGTACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGtt                                                                                                                          <  1:1039774/139‑1 (MQ=255)
                 tACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCAcg                                                                                                                >  2:460012/1‑139 (MQ=255)
                 tACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCAcg                                                                                                                <  1:460012/139‑1 (MQ=255)
                 tACCCACTGAGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCGAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCAcg                                                                                                                <  1:1001066/139‑1 (MQ=255)
                    ccACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCAcgtcg                                                                                                             <  1:632324/139‑1 (MQ=255)
                      aCTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGATACCGGAAGCGCCCCCATGTTGATCGCCACGTCGtt                                                                                                           >  2:392432/1‑139 (MQ=255)
                            cAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCAt                                                                                                     <  2:601360/139‑1 (MQ=255)
                                gtttgttAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCAcc                                                                                                 >  2:625013/1‑139 (MQ=255)
                                      tAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGAcag                                                                                           <  1:980011/139‑1 (MQ=255)
                                         aaCTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGGTCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGAcagcag                                                                                        <  1:1381782/139‑1 (MQ=255)
                                         aaCTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCAt                                                                                                     >  2:1253771/1‑126 (MQ=255)
                                         aaCTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCAt                                                                                                     <  1:1253771/126‑1 (MQ=255)
                                         aaCTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGAcagcag                                                                                        >  2:746722/1‑139 (MQ=255)
                                            tttCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGc                                                                                     <  1:1246173/139‑1 (MQ=255)
                                               ccATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAAACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATg                                                                                  >  1:948688/1‑139 (MQ=255)
                                                   gCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTa                                                                              <  1:7472/139‑1 (MQ=255)
                                                        cTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCATCACCTGACAGCAGAGCATGGTTAATGCCt                                                                        >  2:1351123/1‑139 (MQ=255)
                                                         tGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCt                                                                        <  1:987026/139‑1 (MQ=255)
                                                             aGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTcaca                                                                    <  2:948688/139‑1 (MQ=255)
                                                              gCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGATACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCacac                                                                   <  1:392432/139‑1 (MQ=255)
                                                                 aGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACtgtg                                                               <  1:1351123/139‑1 (MQ=255)
                                                                     cACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCAt                                                                                                     <  1:543473/98‑1 (MQ=255)
                                                                     cACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCAt                                                                                                     >  2:543473/1‑98 (MQ=255)
                                                                                        tgGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTggg                                                          <  1:173847/122‑1 (MQ=255)
                                                                                        tgGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTggg                                                          >  2:173847/1‑122 (MQ=255)
                                                                                        tgGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCAt                                         <  1:746722/139‑1 (MQ=255)
                                                                                             cACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTcaca                                                                    >  2:819210/1‑107 (MQ=255)
                                                                                             cACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTcaca                                                                    <  1:819210/107‑1 (MQ=255)
                                                                                                          aaGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTcaca                                                                    >  2:485429/1‑94 (MQ=255)
                                                                                                          aaGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTcaca                                                                    <  1:485429/94‑1 (MQ=255)
                                                                                                          aaGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTc                       >  1:204219/1‑139 (MQ=255)
                                                                                                                    gttcaAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCg               <  1:965069/137‑1 (MQ=255)
                                                                                                                    gttcaAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCg               >  2:965069/1‑137 (MQ=255)
                                                                                                                               ccGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTcaca                                                                    >  2:1445642/1‑73 (MQ=255)
                                                                                                                               ccGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTcaca                                                                    <  1:1445642/73‑1 (MQ=255)
                                                                                                                               ccGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTc  >  1:377071/1‑139 (MQ=255)
                                                                                                                                        |                                                                                                                                  
CTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTC  >  NC_000913/1685401‑1685667

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: