Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,288,561 (C)7→6 pseudogene (354/2525 nt) yejO ← pseudogene, autotransporter outer membrane homology,putative transport, Not classified, putative ATP‑binding component of a transport system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,288,5550C.98.4% 251.4 / ‑3.0 62pseudogene (360/2525 nt)yejOpseudogene, autotransporter outer membrane homology,putative transport, Not classified, putative ATP‑binding component of a transport system
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base . (32/29);  minor base T (0/1);  total (32/30)
Fisher's exact test for biased strand distribution p-value = 4.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GACGGTTTGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCG  >  NC_000913/2288420‑2288688
                                                                                                                                       |                                                                                                                                     
gaCGGTTTGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGcccc                                                                                                                                    <  2:600095/139‑1 (MQ=255)
gaCGGTTTGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGcccc                                                                                                                                    >  1:600095/1‑139 (MQ=255)
 aCGGTTTGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGccccc                                                                                                                                   >  2:546998/1‑139 (MQ=255)
   ggTTTGTTCCGAATACCCCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGcccccca                                                                                                                                 <  2:1284487/139‑2 (MQ=255)
                   cTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCATCTTTTTCAACGATTa                                                                                                                >  1:451241/1‑139 (MQ=255)
                        tGGCCCGCGTGGTTGTTTTAATATCACCGCCTGCTTTCTGATCTACCACAAATGCAAAGCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATATCGACAAAGACGTCCCCCCATCTTTTTCAACGATTAAgcgc                                                                                                           <  1:1334795/139‑1 (MQ=255)
                         ggCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCATCTTTTTCAACGATTAAGcgcc                                                                                                          <  2:491630/139‑1 (MQ=255)
                         ggCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCATCTTTTTCAACGATTAAGcgcc                                                                                                          <  1:1007809/139‑1 (MQ=255)
                         ggCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCATCTTTTTCAACGATTAAGcgcc                                                                                                          <  2:840380/139‑1 (MQ=255)
                            ccGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGcccc                                                                                                                                    <  2:393545/111‑1 (MQ=255)
                            ccGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGcccc                                                                                                                                    >  1:393545/1‑111 (MQ=255)
                             cgcgTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCATCTTTTTCAACGATTAAGcgcccgcc                                                                                                      >  2:49046/1‑139 (MQ=255)
                              gcgTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCt                                                                                                     >  1:269243/1‑139 (MQ=255)
                               cgTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGcccccc                                                                                                                                  >  1:1033142/1‑110 (MQ=255)
                               cgTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGcccccc                                                                                                                                  <  2:1033142/110‑1 (MQ=255)
                               cgTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCtt                                                                                                    <  2:269243/139‑1 (MQ=255)
                               cgTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCtt                                                                                                    >  2:756038/1‑139 (MQ=255)
                               cgTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCtt                                                                                                    >  1:837865/1‑139 (MQ=255)
                                                 aCCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCg                                                                                  >  2:1147696/1‑139 (MQ=255)
                                                  ccGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGc                                                                                 >  1:1241392/1‑139 (MQ=255)
                                                    gCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTCACCTCCGGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTg                                                                               <  1:449369/139‑1 (MQ=255)
                                                     ccTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTgc                                                                              >  1:1256145/1‑139 (MQ=255)
                                                     ccTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTgc                                                                              >  2:744922/1‑139 (MQ=255)
                                                     ccTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTgc                                                                              >  1:1449787/1‑139 (MQ=255)
                                                     ccTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTg                                                                               <  1:770528/138‑1 (MQ=255)
                                                     ccTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTg                                                                               >  2:770528/1‑138 (MQ=255)
                                                          tttCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCAt                                                                         <  2:1241392/139‑1 (MQ=255)
                                                                 tCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTg                                                                               <  2:30189/126‑1 (MQ=255)
                                                                 tCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTg                                                                               >  1:30189/1‑126 (MQ=255)
                                                                    aCCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAg                                                               <  1:744922/139‑1 (MQ=255)
                                                                      cGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGca                                                             <  1:49046/139‑1 (MQ=255)
                                                                        caaatgcaaatCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGcaca                                                           <  2:1449787/139‑1 (MQ=255)
                                                                        caaatgcaaatCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGcaca                                                           <  2:1322149/139‑1 (MQ=255)
                                                                          aatgcaaatCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTg                                                                                            <  1:1331249/104‑1 (MQ=255)
                                                                          aatgcaaatCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTg                                                                                            >  2:1331249/1‑104 (MQ=255)
                                                                          aatgcaaatCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTg                                                                               <  2:175639/117‑1 (MQ=255)
                                                                          aatgcaaatCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTg                                                                               >  1:175639/1‑117 (MQ=255)
                                                                           atgcaaatCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCa                                                        <  1:1147696/139‑1 (MQ=255)
                                                                               aaatCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCattat                                                    >  2:224157/1‑139 (MQ=255)
                                                                               aaatCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCattat                                                    >  2:845317/1‑139 (MQ=255)
                                                                               aaatCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCattat                                                    <  2:649075/139‑1 (MQ=255)
                                                                                     ccccTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCATCTTTTTCAACGAtt                                                                                                                 <  2:25366/72‑1 (MQ=255)
                                                                                     ccccTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCATCTTTTTCAACGAtt                                                                                                                 >  1:25366/1‑72 (MQ=255)
                                                                                      cccTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGcc                                                                                                                                      >  1:559110/1‑51 (MQ=255)
                                                                                      cccTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGcc                                                                                                                                      <  2:559110/51‑1 (MQ=255)
                                                                                      cccTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCg                                                                                              >  2:1224054/1‑90 (MQ=255)
                                                                                      cccTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCg                                                                                              <  1:1224054/90‑1 (MQ=255)
                                                                                       ccTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTa                                            >  2:765294/1‑139 (MQ=255)
                                                                                             tagccTCCAGTGGTCCTCCCTTTTCGATAGCGACAAAGACGGcccc                                                                                                                                    >  1:850755/4‑46 (MQ=38)
                                                                                             tAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGcccc                                                                                                                                    <  2:850755/46‑1 (MQ=255)
                                                                                                   ccAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGcc                                >  1:236110/1‑139 (MQ=255)
                                                                                                    cAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCcg                               >  2:1246256/1‑139 (MQ=255)
                                                                                                    cAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCcg                               >  1:232364/1‑139 (MQ=255)
                                                                                                    cAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCcg                               >  1:509197/1‑139 (MQ=255)
                                                                                                     agtagtCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCAt                                                                         <  1:247533/96‑1 (MQ=255)
                                                                                                     agtagtCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCAt                                                                         >  2:247533/1‑96 (MQ=255)
                                                                                                     agtagtCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCcgc                              >  1:1443598/1‑139 (MQ=255)
                                                                                                     agtagtCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCcgc                              >  2:909666/1‑139 (MQ=255)
                                                                                                       tagtCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCATCTTTTTCAACGATTAAgcgc                                                                                                           <  2:1054365/60‑1 (MQ=255)
                                                                                                       tagtCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCATCTTTTTCAACGATTAAgcgc                                                                                                           >  1:1054365/1‑60 (MQ=255)
                                                                                                         gtCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCa                          <  1:845317/139‑1 (MQ=255)
                                                                                                          tcctccCTTTTCGATAGCGACAAAGACGGCCCCCTATCTTTTTCAACGATTAAGcgccc                                                                                                         <  1:995258/59‑1 (MQ=39)
                                                                                                          tcctccCTTTTCGATAGCGACAAAGACGGCCCCCCATCTTTTTCAACGATTACGcgccc                                                                                                         >  2:995258/1‑59 (MQ=39)
                                                                                                            ctccCTTTTCGATAGCGACAAAGACGGCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCAccc                       >  1:1351466/1‑139 (MQ=255)
                                                                                                              cccTTTTCGATAGCGACAAAGACGGCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCAt                     >  2:13032/1‑139 (MQ=255)
                                                                                                              cccTTTTCGATAGCGACAAAGACGGCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCAt                     >  2:684566/1‑139 (MQ=255)
                                                                                                                   ttCGATAGCGACAAAGACGGCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGaa                <  2:509197/139‑1 (MQ=255)
                                                                                                                    tCGATAGCGACAAAGACGGCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAAc               <  2:339940/139‑1 (MQ=255)
                                                                                                                         aGCGACAAAGACGGCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAAttt          >  2:179686/1‑139 (MQ=255)
                                                                                                                               aaaGACGGCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAAc    <  2:1227807/139‑1 (MQ=255)
                                                                                                                                 aGACGGCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCg  <  1:765294/139‑1 (MQ=255)
                                                                                                                                 aGACGGCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCg  <  1:684566/139‑1 (MQ=255)
                                                                                                                                 aGACGGCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCg  <  1:546998/139‑1 (MQ=255)
                                                                                                                                 aGACGGCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCg  <  1:179686/139‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                     
GACGGTTTGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCG  >  NC_000913/2288420‑2288688

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: