Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,499,517 (A)5→4 coding (847/1698 nt) ypdA → sensor kinase regulating yhjX, pyruvate‑responsive YpdAB two‑component system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,499,5130A.100.0% 360.4 / NA 85coding (843/1698 nt)ypdAsensor kinase regulating yhjX, pyruvate‑responsive YpdAB two‑component system
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (44/41);  total (44/41)

TGACATTCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGTGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGAAAAATCATCATTAAAAACAATGATGAAGCCCACCGCACACCAGAGATTCATTCCATGCTGGTGATCCCATTGTGGGAGAAAGGGGTCGTGACCGGAACGCTGAAAATTTACTACTGCCACGCGCATCAGATCAC  >  NC_000913/2499381‑2499647
                                                                                                                                    |                                                                                                                                      
tGACATTCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGTGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGaaaatca                                                                                                                                  <  1:201102/139‑4 (MQ=255)
tGACATTCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGTGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGaaaatca                                                                                                                                  <  2:362914/139‑4 (MQ=255)
     ttCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGTGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGAAAATCATCAtt                                                                                                                            <  1:1464283/139‑1 (MQ=255)
     ttCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGTGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGAAAATCATCAtt                                                                                                                            <  1:1380305/139‑1 (MQ=255)
          gCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGTGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGAAAATCATCATTaaaaa                                                                                                                       <  2:1445243/139‑1 (MQ=255)
              aTGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGTGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGAAAATCATCATTAAAAACAat                                                                                                                   >  2:1476781/1‑139 (MQ=255)
                 cGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGTGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGAAAATCATCATTAAAAACAatgat                                                                                                                <  2:1192844/139‑1 (MQ=255)
                  ggTGGCGTTTACTAATACCGATCATGTGCTGGCCTATGTTGGCGTGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGTGATGAATTACGGAAAATCATCATTAAAAACAatgatg                                                                                                               <  1:23482/139‑1 (MQ=255)
                            aCTAATACCGATCATGTGCTGGCCTATGTTGGCGTGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGAAAATCATCATTAAAAACAATGATGAAGCCCACCg                                                                                                     <  1:1476781/139‑1 (MQ=255)
                                   ccGATCATGTGCTGGCCTATGTTGGCGTGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGAAAATCATCATTAAAAACAATGATGAAGCCCACCGCACACCa                                                                                              <  2:200542/139‑1 (MQ=255)
                                     gATCATGTGCTGGCCTATGTTGGCGTGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGAAAATCATCATTAAAAACAatgat                                                                                                                <  2:191902/119‑1 (MQ=255)
                                     gATCATGTGCTGGCCTATGTTGGCGTGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGAAAATCATCATTAAAAACAatgat                                                                                                                >  1:191902/1‑119 (MQ=255)
                                      aTCATGTGCTGGCCTATGTTGGCGTGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGaaaat                                                                                                                                    <  1:892023/99‑2 (MQ=255)
                                      aTCATGTGCTGGCCTATGTTGGCGTGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGAAAATCATCATTaaaaa                                                                                                                       <  1:116913/111‑1 (MQ=255)
                                      aTCATGTGCTGGCCTATGTTGGCGTGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGAAAATCATCATTaaaaa                                                                                                                       >  2:116913/1‑111 (MQ=255)
                                      aTCATGTGCTGGCCTATGTTGGCGTGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGAAAATCATCATTaaa                                                                                                                         <  2:1045592/109‑1 (MQ=255)
                                      aTCATGTGCTGGCCTATGTTGGCGTGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGAAAATCATCATTaaa                                                                                                                         >  1:1045592/1‑109 (MQ=255)
                                      aTCATGTGCTGGCCTATGTTGGCGTGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGAAAATCATCATTAAAAACAATGATGACGCCCACCGCACACCagag                                                                                           >  2:988229/1‑139 (MQ=255)
                                      aTCATGTGCTGGCCTATGTTGGCGTGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGAAAATCATCATTAAAAACAATGATGAAGCCCACCGCACACCagag                                                                                           >  2:1131511/1‑139 (MQ=255)
                                      aTCATGTGCTGGCCTATGTTGGCGTGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGAAAATCATCATTAAAAACAATGATGAAGCCCACCGCACACCagag                                                                                           >  1:575995/1‑139 (MQ=255)
                                      aTCATGTGCTGGCCAATGTTGGCGTGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTAGCCGTCAGGCGATGAATTACGGaaaat                                                                                                                                    >  2:892023/1‑98 (MQ=255)
                                       tCATGTGCTGGCCTATGTTGGCGTGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGAAAATCATCATTAAAAACAATGATGAAGCCCACCGCACACCAgaga                                                                                          >  2:758085/1‑139 (MQ=255)
                                             gCTGGCCTATGTTGGCGTGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGAAAATCATCATTAAAAACAATGATGAAGCCCACCGCACACCAGAGattcatt                                                                                    <  1:988229/139‑1 (MQ=255)
                                                 gCCTATGTTGGCGTGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGaaaatca                                                                                                                                  <  2:909386/90‑4 (MQ=255)
                                                 gCCTATGTTGGCGTGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGaaaatca                                                                                                                                  >  1:909386/1‑87 (MQ=255)
                                                 gCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGAAAATCATCATTAAAAACAATGATGAAGCCCACCGCACACCAGAGATTCATTCCAt                                                                                >  2:1040619/1‑139 (MQ=255)
                                                      tGTTGGCGTGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGAAAATCATCATTAAAAACAATGATGAAGCCCACCGCACACCAGAGATTCATTCCATGCTgg                                                                           <  1:1403745/139‑1 (MQ=255)
                                                           gCGTGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGAAAATCATCATTAAAAACAATGATGAAGc                                                                                                           >  2:420707/1‑102 (MQ=255)
                                                           gCGTGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGAAAATCATCATTAAAAACAATGATGAAGc                                                                                                           <  1:420707/102‑1 (MQ=255)
                                                           gCGTGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGAAAATCATCATTAAAAACAATGATGAAGCCCACCGCACACCAGAGATTCATTCCATGCTGGTGAt                                                                       <  1:83023/138‑1 (MQ=255)
                                                           gCGTGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGAAAATCATCATTAAAAACAATGATGAAGCCCACCGCACACCAGAGATTCATTCCATGCTGGTGAt                                                                       >  2:83023/1‑138 (MQ=255)
                                                           gCGTGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGAAAATCATCATTAAAAACAATGATGAAGCCCACCGCACACCAGAGATTCATTCCATGCTGGTGATc                                                                      >  2:1079513/1‑139 (MQ=255)
                                                           gCGTGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGAAAATCATCATTAAAAACAATGATGAAGCCCACCGCACACCAGAGATTCATTCCATGCTGGTGATc                                                                      >  2:383054/1‑139 (MQ=255)
                                                                ggTGAACATAACTATCATAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGAAAATCATCATTAAAAACAATGATGAAGCCCACCGca                                                                                                   >  2:598963/1‑105 (MQ=255)
                                                                ggTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGAAAATCATCATTAAAAACaa                                                                                                                    >  2:751580/1‑88 (MQ=255)
                                                                ggTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGAAAATCATCATTAAAAACaa                                                                                                                    <  1:751580/88‑1 (MQ=255)
                                                                ggTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGAAAATCATCATTAAAAACAATGATGAAGCCCACCGca                                                                                                   <  1:598963/105‑1 (MQ=255)
                                                                ggTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGAAAATCATCATTAAAAACAATGATGAAGCCCACCGCACACCAGAGATTCATTCCATGCTGGTGATCCCAtt                                                                 >  2:942004/1‑139 (MQ=255)
                                                                  tGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGAAAATCATCATTAAAAACAATGATGAAGCCCACCGCACACCAGAGATTCATTCCATGCTGGTGATCCCATtgt                                                               <  1:61052/139‑1 (MQ=255)
                                                                    aaCATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGaaaatca                                                                                                                                  >  1:502492/1‑68 (MQ=255)
                                                                    aaCATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGaaaatca                                                                                                                                  <  2:502492/71‑4 (MQ=255)
                                                                          aCTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGAAAATCATCATTAAAAACAATGATGAAGCCCACCGCACACCa                                                                                              >  2:452698/1‑100 (MQ=255)
                                                                          aCTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGAAAATCATCATTAAAAACAATGATGAAGCCCACCGCACACCa                                                                                              <  1:452698/100‑1 (MQ=255)
                                                                           cTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGAAAATCATCATTAAAAACAATGATGAAGCCCACCGCACACCAGAGATTCATTCCATGCTGGTGATCCCATTGTGGGAGAAAg                                                      <  2:528963/139‑1 (MQ=255)
                                                                                aGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGAAAATCATCATTAAAAACAATGATGAAGCCCACCGCACACCAGAGATTCATTCCATGCTGGTGATCCCATTGTGGGAGAAAGGGGTc                                                 >  1:353415/1‑139 (MQ=255)
                                                                                   aTGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGAAAATCATCATTAAAAACAATGATGAAGCCCACCGCACACCa                                                                                              >  1:1455497/1‑91 (MQ=255)
                                                                                   aTGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGAAAATCATCATTAAAAACAATGATGAAGCCCACCGCACACCa                                                                                              <  2:1455497/91‑1 (MQ=255)
                                                                                       cGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGAAAATCATCATTAAAAACAATGATGAAGCCCACCGCACACCAGAGat                                                                                         >  1:1210970/1‑92 (MQ=255)
                                                                                       cGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGAAAATCATCATTAAAAACAATGATGAAGCCCACCGCACACCAGAGat                                                                                         <  2:1210970/92‑1 (MQ=255)
                                                                                        gATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGAAAATCATCATTAAAAACAATGATGAAGCCCACCg                                                                                                     >  2:671796/1‑79 (MQ=255)
                                                                                        gATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGAAAATCATCATTAAAAACAATGATGAAGCCCACCg                                                                                                     <  1:671796/79‑1 (MQ=255)
                                                                                        gATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGAAAATCATCATTAAAAACAATGATGAAGCCCACCg                                                                                                     <  1:1116062/79‑1 (MQ=255)
                                                                                        gATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGAAAATCATCATTAAAAACAATGATGAAGCCCACCg                                                                                                     >  2:1116062/1‑79 (MQ=255)
                                                                                          tGACTTCATTAGCCCGACTACCCGTCAGGCGATGAAGTACGGAAAATCATCATTAAAAACAATGATGAAGCCCACCGCACACCAGAGATTCATTCCATGCTGGTGATCCCATTGTGGGAGAAAGGGGTCGTGACCGGaa                                       <  1:1040619/139‑1 (MQ=255)
                                                                                            aCTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGAAAATcatcat                                                                                                                             >  1:876310/1‑51 (MQ=255)
                                                                                            aCTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGAAAATcatcat                                                                                                                             <  2:876310/51‑1 (MQ=255)
                                                                                            aCTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGAAAATCATCATTAAAAACAATGATGAAGCCCACCGCACACCAGAGATTCATTCCATGCTGGTGATCCCATTGTGGGAGAAAGGGGTCGTGACCGGAACg                                     >  2:669290/1‑139 (MQ=255)
                                                                                             cTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGAAAATCATCATTAAAAACAATGATGAAGCCCACCGCACACCAGAGATTCATTCCATGCTGGTGATCCCATTGTGGGAGAAAGGGGTCGTGACCGGAACGc                                    <  2:575995/139‑1 (MQ=255)
                                                                                                cATTAGCCCGACTACCCGTCAGGCGATGAATTACGGaaaatca                                                                                                                                  <  1:244875/43‑4 (MQ=255)
                                                                                                cATTAGCCCGACTACCCGTCAGGCGATGAATTACGGaaaatca                                                                                                                                  >  2:244875/1‑40 (MQ=255)
                                                                                                cATTAGCCCGACTACCCGTCAGGCGATGAATTACGGAAAATCATCATTAAAAACAATGATGAAGCCCACCGCACACCAGAGATTCATTCCATGCTGGTGATCCCATtg                                                                <  1:234503/108‑1 (MQ=255)
                                                                                                cATTAGCCCGACTACCCGTCAGGCGATGAATTACGGAAAATCATCATTAAAAACAATGATGAAGCCCACCGCACACCAGAGATTCATTCCATGCTGGTGATCCCATtg                                                                >  2:234503/1‑108 (MQ=255)
                                                                                                cATTAGCCCGACTACCCGTCAGGCGATGAATTACGGAAAATCATCATTAAAAACAATGATGAAGCCCACCGCACACCAGAGATTCATTCCATGCTGGTGATCCCATTGTGGGAGAAAGGGGTCGTGACCGGAACGCTGa                                 >  1:1347522/1‑139 (MQ=255)
                                                                                                cATTAGCCCGACTACCCGTCAGGCGATGAATTACGGAAAATCATCATTAAAAACAATGATGAAGCCCACCGCACACCAGAGATTCATTCCATGCTGGTGATCCCATTGTGGGAGAAAGGGGTCGTGACCGGAACGCTGa                                 <  1:383054/139‑1 (MQ=255)
                                                                                                      cccGACTACCCGTCAGGCGATGAATTACGGAAAATCATCATTAAAAACAATGATGAAg                                                                                                            >  2:741298/1‑58 (MQ=255)
                                                                                                      cccGACTACCCGTCAGGCGATGAATTACGGAAAATCATCATTAAAAACAATGATGAAg                                                                                                            <  1:741298/58‑1 (MQ=255)
                                                                                                      cccGACTACCCGTCAGGCGATGAATTACGGAAAATCATCATTAAAAACAATGATGAAGcc                                                                                                          >  2:771799/1‑60 (MQ=255)
                                                                                                      cccGACTACCCGTCAGGCGATGAATTACGGAAAATCATCATTAAAAACAATGATGAAGcc                                                                                                          <  1:771799/60‑1 (MQ=255)
                                                                                                      cccGACTACCCGTCAGGCGATGAATTACGGAAAATCATCATTAAAAACAATGATGAAGCCCACCGCACACCAGAGATTCATTCCATGCTgg                                                                           <  1:596505/91‑1 (MQ=255)
                                                                                                      cccGACTACCCGTCAGGCGATGAATTACGGAAAATCATCATTAAAAACAATGATGAAGCCCACCGCACACCAGAGATTCATTCCATGCTgg                                                                           >  2:596505/1‑91 (MQ=255)
                                                                                                      cccGACTACCCGTCAGGCGATGAATTACGGAAAATCATCATTAAAAACAATGATGAAGCCCACCGCACACCAGAGATTCATTCCATGCTGGTGATCCCATTGTGGGAGAAAGGGGTCGTGACCGGAACGCTGAAAAttt                           >  1:810838/1‑139 (MQ=255)
                                                                                                        cGACTACCCGTCAGGCGATGAATTACGGAAAATCATCATTAAAAACAATGATGAAGcc                                                                                                          >  1:447528/1‑58 (MQ=255)
                                                                                                        cGACTACCCGGCAGGCGATGAATTACGGAAAATCATCATTAAAAACAATGATGAAGcc                                                                                                          <  2:447528/58‑1 (MQ=255)
                                                                                                          aCTACCCGTCAGGCGATGAATTACGGAAAATCGTCATTAAAAACAATGATGAAGCCCACCGCACACCAGAGATTCATTCCATGCTGGTGATCCCATTGTGGGAGAAAGGGGTCGTGACCGGAACGCTGAAAATTtacta                       >  1:332057/1‑139 (MQ=255)
                                                                                                          aCTACCCGTCAGGCGATGAATTACGGAAAATCATCATTAAAAACAATGATGAAGCCCACCGCACACCAGAGATTCATTCCa                                                                                 >  2:190601/1‑81 (MQ=255)
                                                                                                          aCTACCCGTCAGGCGATGAATTACGGAAAATCATCATTAAAAACAATGATGAAGCCCACCGCACACCAGAGATTCATTCCa                                                                                 <  1:190601/81‑1 (MQ=255)
                                                                                                          aCTACCCGTCAGGCGATGAATTACGGAAAATCATCATTAAAAACAATGATGAAGCCCACCGCACACCAGAGATTCATTCCATGCTGGTGATCCCATTGTGGGAGAAAGGGGTCGTGACCGGAACGCTGAAAATTtacta                       >  2:1294386/1‑139 (MQ=255)
                                                                                                          aCTACCCGTCAGGCGATGAATTACGGAAAATCATCATTAAAAACAATGATGAAGCCCACCGCACACCAGAGATTCATTCCATGCTGGTGATCCCATTGTGGGAGAAAGGGGTCGTGACCGGAACGCTGAAAATTtacta                       >  2:422403/1‑139 (MQ=255)
                                                                                                          aCTACCCGTCAGGCGATGAATTACGGAAAATCATCATTAAAAACAATGATGAAGCCCACCGCACACCAGAGATTCATTCCATGCTGGTGATCCCATTGTGGGAGAAAGGGGTCGTGACCGGAACGCTGAAAATTtacta                       >  2:375063/1‑139 (MQ=255)
                                                                                                          aCTACCCGTCAGGCGATGAATTACGGAAAATCATCATTAAAAACAATGATGAAGCCCACCGCACACCAGAGATTCATTCCATGCTGGTGATCCCATTGTGGGAGAAAGGGGTCGTGACCGGAACGCTGAAAATTtacta                       >  2:375015/1‑139 (MQ=255)
                                                                                                          aCTACCCGTCAGGCGATGAATTACGGAAAATCATCATTAAAAACAATGATGAAGCCCACCGCACACCAGAGATTCATTCCATGCTGGTGATCCCATTGTGGGAGAAAGGGGTCGTGACCGGAACGCTGAAAATTtacta                       >  1:1096148/1‑139 (MQ=255)
                                                                                                          aCTACCCGTCAGGCGATGAATTACGGAAAATCATCATTAAAAACAATGATGAAGCCCACCGCACACCAGAGATTCATTCCATGCTGGTGATCCCATTGTGGGAGAAAGGGGTCGTGACCGGAACGCTGAAAATTtacta                       >  1:640843/1‑139 (MQ=255)
                                                                                                           cTACCCGTCAGGCGATGAATTACGGAAAATCATCATTAAAAACaa                                                                                                                    >  1:746768/1‑45 (MQ=39)
                                                                                                           cTACCCGTCAGGCGATGAATTACGGAAAATCATCATTAAAAACaa                                                                                                                    <  2:746768/45‑1 (MQ=39)
                                                                                                               ccGTCAGGCGATGAATTACGGAAAATCATCATTAAAAACAATGATGAAGCCCACCGCACACCAGAGATTCATTCCATGCTGGTGATCCCATTGTGGGAGAAAGGGGTCGTGACCGGAACGCTGAAAATTTACTACTGcc                  <  2:1347522/139‑1 (MQ=255)
                                                                                                               ccGTCAGGCGATGAATTACGGAAAATCATCATTAAAAACAATGATGAAGCCCACCGCACACCAGAGATTCATTCCATGCTGGTGATCCCATTGTGGGAGAAAGGGGTCGTGACCGGAACGCTGAAAATTTACTACTGcc                  >  1:277894/1‑139 (MQ=255)
                                                                                                                 gTCAGGCGATGAATTACGGAAAATCATCATTAAAAACAATGATGAAGCCCACCGCACACCAGAGATTCATTCCATGCTGGTGATCCCATTGTGGGAGAAAGGGGTCGTGACCGGAACGCTGAAAATTTACTACTGCCAc                >  2:43098/1‑139 (MQ=255)
                                                                                                                  tCAGGCGATGAATTACGGAAAATCATCATTAAAAACAATGATGAAGCCCACCGCACACCAGAGATTCATTCCATGCTGGTGATCCCATTGTGGGAGAAAGGGGTCGTGACCGGAACGCTGAAAATTTACTACTGCCAcg               <  1:422403/139‑1 (MQ=255)
                                                                                                                  tCAGGCGATGAATTACGGAAAATCATCATTAAAAACAATGATGAAGCCCACCGCACACCAGAGATTCATTCCATGCTGGTGATCCCATTGTGGGAGAAAGGGGTCGTGACCGGAACGCTGAAAATTTACTACTGCCAcg               <  2:353415/139‑1 (MQ=255)
                                                                                                                         aTGAATTACGGAAAATCATCATTAAAAACAATGATGAAGCCCACCGCACACCAGAGattcatt                                                                                    <  2:123696/63‑1 (MQ=255)
                                                                                                                         aTGAATTACGGAAAATCATCATTAAAAACAATGATGAAGCCCACCGCACACCAGAGattcatt                                                                                    >  1:123696/1‑63 (MQ=255)
                                                                                                                         aTGAATTACGGAAAATCATCATTAAAAACAATGATGAAGCCCACCGCACACCAGAGATTCATTCCATGCTGGTGATCCCATTGTgg                                                             <  2:244145/86‑1 (MQ=255)
                                                                                                                         aTGAATTACGGAAAATCATCATTAAAAACAATGATGAAGCCCACCGCACACCAGAGATTCATTCCATGCTGGTGATCCCATTGTgg                                                             >  1:244145/1‑86 (MQ=255)
                                                                                                                          tGAATTACGGAAAATCATCATTAAAAACAATGATGAAGCCCACCGCACACCAGAGATTCATTCCATGCTGGTGATCCCATTGTGGGAGAAAGGGGTCGTGACCGGAACGCTGAAAATTTACTACTGCCACGCGCATCAg       <  1:375063/139‑1 (MQ=255)
                                                                                                                               tACGGAAAATCATCATTAAAAACAATGATGAAGCCCACCGCACACCAGAGATTCATTCCATGCTGGTGATCCCATTGTGGGAGAAAGGGGTCGTGACCGGAACGCTGAAAATTTACTACTGCCACGCGCATCAGATCAc  <  1:1378244/139‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                      
TGACATTCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGTGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGAAAAATCATCATTAAAAACAATGATGAAGCCCACCGCACACCAGAGATTCATTCCATGCTGGTGATCCCATTGTGGGAGAAAGGGGTCGTGACCGGAACGCTGAAAATTTACTACTGCCACGCGCATCAGATCAC  >  NC_000913/2499381‑2499647

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: