Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,014,259 Δ1 bp intergenic (‑20/+28) yqeB ← / ← yqeC XdhC‑CoxI family protein with NAD(P)‑binding Rossman fold/putative selenium‑dependent hydroxylase accessory protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,014,2580C.94.4% 207.2 / 5.9 54intergenic (‑19/+29)yqeB/yqeCXdhC‑CoxI family protein with NAD(P)‑binding Rossman fold/putative selenium‑dependent hydroxylase accessory protein
Reads supporting (aligned to +/- strand):  ref base C (3/0);  new base . (25/26);  total (28/26)
Fisher's exact test for biased strand distribution p-value = 2.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.04e-01

GCGCACTAACATTTGTGCAGAATGGCGGGGAGTCGAGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCGCTTTGTG  >  NC_000913/3014123‑3014390
                                                                                                                                       |                                                                                                                                    
gcgcACTAACATTTGTGCAGAATGGCGGGGAGTCGAGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCttt                                                                                                                                   >  1:790743/1‑139 (MQ=255)
 cgcACTAACATTTGTGCAGAATGGCGGGGAGTCGAGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCtttc                                                                                                                                  >  2:1381176/1‑138 (MQ=255)
   cACTAACATTTGTGCAGAATGGCGGGGAGTCGAGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCTTTCAg                                                                                                                               <  2:689329/139‑1 (MQ=255)
                gCAGAATGGCGGGGAGTCGAGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCTTTCAg                                                                                                                               <  2:881207/126‑1 (MQ=255)
                gCAGAATGGCGGGGAGTCGAGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCTTTCAg                                                                                                                               >  1:881207/1‑126 (MQ=255)
                      tGGCGGGGAGTCGAGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCTTTCAGTAACCCGGAATACCCGGGc                                                                                                            <  1:859393/139‑1 (MQ=255)
                         cGGGGAGTCTAGCCTCGGCTATCAACAATTTGCGACATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCTTTCAGTAACCCGGAATACCCGGGCCgt                                                                                                         <  2:941700/139‑2 (MQ=255)
                                tCGAGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTca                                                                                                  >  1:183793/1‑139 (MQ=255)
                                         ggCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATcttt                                                                                                                                   <  1:1293212/98‑4 (MQ=255)
                                         ggCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCttt                                                                                                                                   >  2:1293212/1‑98 (MQ=255)
                                          gCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTgcg                                                                                        >  2:668145/1‑139 (MQ=255)
                                                  caaTTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTg                                                                                <  1:1381176/139‑1 (MQ=255)
                                                    aTTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCa                                                                              <  1:1134336/139‑1 (MQ=255)
                                                       tGCGCCATACCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGa                                                                           <  2:131787/139‑1 (MQ=255)
                                                        gcgcCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGAt                                                                          >  1:439936/1‑139 (MQ=255)
                                                        gcgcCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGAt                                                                          >  2:144689/1‑139 (MQ=255)
                                                         cgcCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATg                                                                         <  2:790743/139‑1 (MQ=255)
                                                         cgcCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATg                                                                         <  2:439936/139‑1 (MQ=255)
                                                         cgcCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATg                                                                         <  2:408189/139‑1 (MQ=255)
                                                             aTAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCt                                                                     >  1:1363115/1‑139 (MQ=255)
                                                               aGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCt                                                                     >  1:1069557/1‑137 (MQ=255)
                                                               aGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCt                                                                     <  2:1069557/137‑1 (MQ=255)
                                                                   aaCGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTgcgcg                                                                                      <  2:1469606/116‑1 (MQ=255)
                                                                   aaCGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTgcgcg                                                                                      >  1:1469606/1‑116 (MQ=255)
                                                                      ggACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCg                                                            <  1:144689/139‑1 (MQ=255)
                                                                                      cGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCct                                            >  1:421105/1‑139 (MQ=255)
                                                                                            ttGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAgg                                                                            >  1:259784/1‑101 (MQ=255)
                                                                                            ttGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAgg                                                                            <  2:259784/101‑1 (MQ=255)
                                                                                            ttGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTActt                                      >  2:796995/1‑137 (MQ=255)
                                                                                                        tgAAAATATTCATACCAACCTCAATGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCa                          <  2:660004/139‑1 (MQ=255)
                                                                                                            aaTATTCATACCAACCTCAATGGATATCTTTCAGTAACCCGGAATACCCGGGCCgtta                                                                                                       >  2:359458/1‑55 (MQ=255)
                                                                                                            aaTATTCATACCAACCTCAATGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTa                                                                                                       <  1:359458/58‑1 (MQ=255)
                                                                                                            aaTATTCATACCAACCTCAATGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAgg                                                                            <  2:741714/85‑1 (MQ=255)
                                                                                                            aaTATTCATACCAACCTCAATGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAgg                                                                            >  1:741714/1‑85 (MQ=255)
                                                                                                                ttCATACCAACCTCAATGGATATCTTTCAGTAACCCGGAATACCCGGGCCgtta                                                                                                       <  2:409523/54‑4 (MQ=255)
                                                                                                                ttCATACCAACCTCAATGGATATCTTTCAGTAACCCGGAATACCCGGGCCgtta                                                                                                       >  1:409523/1‑51 (MQ=255)
                                                                                                                  cATACCAACCTCAATGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTgcgcg                                                                                      >  2:436911/1‑69 (MQ=255)
                                                                                                                  cATACCAACCTCAATGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTgcgcg                                                                                      <  1:436911/69‑1 (MQ=255)
                                                                                                                  cATACCAACCTCAATGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGAt                                                                          <  1:155813/81‑1 (MQ=255)
                                                                                                                  cATACCAACCTCAATGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGAt                                                                          >  2:155813/1‑81 (MQ=255)
                                                                                                                  cATACCAACCTCAATGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTAcgt                                      >  1:824354/1‑117 (MQ=255)
                                                                                                                  cATACCAACCTCAATGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTAcgt                                      <  2:824354/117‑1 (MQ=255)
                                                                                                                     aCCAACCTCAATGTATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAgg                                                                            <  1:118166/76‑1 (MQ=255)
                                                                                                                     aCCAACCTCAATGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAgg                                                                            >  2:118166/1‑76 (MQ=255)
                                                                                                                        aaCCTCAATGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCa                                                                              >  1:1261151/1‑71 (MQ=255)
                                                                                                                        aaCCTCAATGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCa                                                                              <  2:1261151/71‑1 (MQ=255)
                                                                                                                         aCCTCAATGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTAcgtcg                                    <  1:615146/112‑1 (MQ=255)
                                                                                                                         aCCTCAATGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTAcgtcg                                    >  2:615146/1‑112 (MQ=255)
                                                                                                                         aCCTCAATGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCg         >  2:117073/1‑139 (MQ=255)
                                                                                                                          ccTCAATGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCGc        >  1:1174907/1‑139 (MQ=255)
                                                                                                                          ccTCAATGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTATATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGCAGGAGCTCGc        >  1:889683/1‑139 (MQ=255)
                                                                                                                              aaTGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCGCTTtg    >  1:1204982/1‑139 (MQ=255)
                                                                                                                               aTGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGa                                                                           <  2:260074/67‑1 (MQ=255)
                                                                                                                               aTGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGa                                                                           >  1:260074/1‑67 (MQ=255)
                                                                                                                                tGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCGCTTtgtg  <  1:117073/139‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                    
GCGCACTAACATTTGTGCAGAATGGCGGGGAGTCGAGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCGCTTTGTG  >  NC_000913/3014123‑3014390

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: