Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,424,543	Δ1 bp	coding (249/276 nt)	yjfY ←	YhcN family protein, periplasmic

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,424,543	0	C	.	100.0%	204.4 / NA	49	coding (249/276 nt)	yjfY	YhcN family protein, periplasmic
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (27/22);  total (27/22)

TGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGC  >  NC_000913/4424425‑4424676                                                                                                                       |                                                                                                                                      tGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTG‑ATATTGCCGTTACTCCCCGGt                                                                                                                  <  1:924999/139‑1 (MQ=255)         ttCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTG‑ATATTGCCGTTACTCCCCGGTTCCCGCAt                                                                                                          >  1:466200/1‑139 (MQ=255)              cAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTG‑ATATTGCCGTTACTCCCCGGTTCCCGCATCAGaa                                                                                                     >  1:113889/1‑139 (MQ=255)                   gTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTG‑ATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACg                                                                                                <  2:1093795/139‑1 (MQ=255)                   gTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTG‑ATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACg                                                                                                <  2:113889/139‑1 (MQ=255)                   gTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTG‑ATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACg                                                                                                <  1:404605/139‑1 (MQ=255)                           aaaaaaaGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTG‑ATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACg                                                                                                <  1:599017/131‑1 (MQ=255)                           aaaaaaaGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTG‑ATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACg                                                                                                >  2:599017/1‑131 (MQ=255)                                    ccccTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTG‑ATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGcc                                                                               <  2:466200/139‑1 (MQ=255)                                                 ggCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTG‑ATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTa                                                                                                  >  1:326309/1‑107 (MQ=255)                                                 ggCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTG‑ATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTa                                                                                                  <  2:326309/107‑1 (MQ=255)                                                                ggCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTG‑ATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACg                                                                                                <  2:356648/94‑1 (MQ=255)                                                                ggCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTG‑ATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACg                                                                                                >  1:356648/1‑94 (MQ=255)                                                                       tttCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTG‑ATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTttct                                                               <  2:1170391/120‑1 (MQ=255)                                                                       tttCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTG‑ATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTttct                                                               >  1:1170391/1‑120 (MQ=255)                                                                         tCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTG‑ATATTGCCGTTACTCCCCGGTTCCCACATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCt                                          >  1:676257/1‑139 (MQ=255)                                                                               gTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTG‑ATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACg                                                                                                >  1:313908/1‑79 (MQ=255)                                                                               gTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTG‑ATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACg                                                                                                <  2:313908/79‑1 (MQ=255)                                                                                         gtgttAGCGATAAATATCCGCGCTGGCGTG‑ATATTGCCGTTACTCCCCGGTTCCCGCa                                                                                                           <  1:933341/54‑1 (MQ=255)                                                                                         gtgttAGCGATAAATATCCGCGCTGGCGTG‑ATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCAttt                                                         <  1:1052748/104‑1 (MQ=255)                                                                                         gtgttAGCGATAAATATCCGCGCTGGCGTG‑ATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGa                          >  1:467555/5‑139 (MQ=255)                                                                                         gtgttAGCGATAAATATCCGCGCTGGCGTG‑ATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGa                          >  2:245361/5‑139 (MQ=255)                                                                                         gtgtTAGCGATAAATATCCGCGCTGGCGTG‑ATATTGCCGTTACTCCCCGGTTCCCGCa                                                                                                           >  2:933341/1‑58 (MQ=255)                                                                                         gtgtTAGCGATAAATATCCGCGCTGGCGTG‑ATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCAttt                                                         >  2:1052748/1‑108 (MQ=255)                                                                                          tgttAGCGATAAATATCCGCGCTGGCGTG‑ATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGc                            >  1:64812/4‑136 (MQ=255)                                                                                          tgttAGCGATAAATATCCGCGCTGGCGTG‑ATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGc                            <  2:64812/133‑1 (MQ=255)                                                                                             tAGCGATAAATATCCGCGCTGGCGTG‑ATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAAtt                      >  2:43688/1‑139 (MQ=255)                                                                                               gCGATAAATATCCGCGCTGGCGTG‑ATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACg                                                                                                <  1:402761/63‑1 (MQ=255)                                                                                               gCGATAAATATCCGCGCTGGCGTG‑ATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACg                                                                                                >  2:402761/1‑63 (MQ=255)                                                                                                 gATAAATATCCGCGCTGGCGTG‑ATATTGCCGTTACTCCCCGGTTCCCACATCAGAATTACGAGGTAGTACGTTGCGACTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTAtga                  <  2:676257/139‑1 (MQ=255)                                                                                                  aTAAATATCCGCGCTGGCGTG‑ATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTAtgat                 <  1:245361/139‑1 (MQ=255)                                                                                                   tAAATATCCGCGCTGGCGTG‑ATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCt                                          >  1:475455/1‑113 (MQ=255)                                                                                                   tAAATATCCGCGCTGGCGTG‑ATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCt                                          <  2:475455/113‑1 (MQ=255)                                                                                                   tAAATATCCGCGCTGGCGTG‑ATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGa                          <  1:833566/129‑1 (MQ=255)                                                                                                   tAAATATCCGCGCTGGCGTG‑ATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGa                          >  2:833566/1‑129 (MQ=255)                                                                                                   tAAATATCCGCGCTGGCGTG‑ATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTAtgatt                >  2:875139/1‑139 (MQ=255)                                                                                                   tAAATATCCGCGCTGGCGTG‑ATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTAtgatt                >  2:481046/1‑139 (MQ=255)                                                                                                          ccGCGCTGGCGTG‑ATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCa                                                                        >  1:1362333/1‑76 (MQ=255)                                                                                                          ccGCGCTGGCGTG‑ATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCa                                                                        <  2:1362333/76‑1 (MQ=255)                                                                                                           cgcgCTGGCGTG‑ATATTGGCGTTACTCCCCGGTTCCCGCATCAGAATTACg                                                                                                >  1:815072/1‑51 (MQ=38)                                                                                                           cgcgCTGGCGTG‑ATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACg                                                                                                <  2:815072/51‑1 (MQ=255)                                                                                                           cgcgCTGGCGTG‑ATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATaaa        >  2:797405/1‑139 (MQ=255)                                                                                                             cgcTGGCGTG‑ATATTGCCGTTACTCCCCGGTTCCCGc                                                                                                            <  1:151770/37‑1 (MQ=37)                                                                                                             cgcTGGCGTG‑ATATTGCCGTTACTCCCCGGTTCCCGc                                                                                                            >  2:151770/1‑37 (MQ=37)                                                                                                             cgcTGGCGTG‑ATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATc      >  1:683877/1‑139 (MQ=255)                                                                                                               cTGGCGTG‑ATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTAtgat                 <  2:474119/126‑1 (MQ=255)                                                                                                               cTGGCGTG‑ATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTAtgat                 >  1:474119/1‑126 (MQ=255)                                                                                                               cTGGCGTG‑ATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCAc    >  2:497786/1‑139 (MQ=255)                                                                                                                 ggCGTG‑ATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGc  >  2:631742/1‑139 (MQ=255)                                                                                                                       |                                                                                                                                      TGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGC  >  NC_000913/4424425‑4424676

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑