| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,250,629 | (C)5→6 | coding (210/1071 nt) | dhaK ← | dihydroxyacetone kinase, PTS‑dependent, dihydroxyacetone‑binding subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,250,624 | 1 | . | C | 95.8% | 158.6 / ‑0.3 | 48 | coding (215/1071 nt) | dhaK | dihydroxyacetone kinase, PTS‑dependent, dihydroxyacetone‑binding subunit |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base C (19/27); total (20/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01 | |||||||||||
GGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAG‑G‑C‑CCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCGGGTGACATACACCGGATCCTGATG > NC_000913/1250488‑1250754 | ggTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAG‑G‑c‑c > 1:790302/1‑139 (MQ=255) tCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAG‑G‑c‑ccc > 1:708101/1‑139 (MQ=255) cgAATATCGCCGGTGTAATTTTTGATAATGAACAGTACACCTTCGCCGCCATCAGCGTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAG‑GCC‑CCCCGAAAGCATCCCCTGa < 1:55292/138‑1 (MQ=255) aGAATATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAG‑GCC‑CCCCGAAAGCATCCCCTGa < 2:137026/139‑1 (MQ=255) aGAATATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAG‑G‑CTCCCCGAAAGCATCCCCTGa < 1:792594/139‑1 (MQ=255) aaTATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAG‑GCC‑CCCCGAAAGCATCCCCTGAcc < 2:708101/139‑1 (MQ=255) aaTATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAG‑GCC‑CCCCGAAAGCATCCCCTGAcc < 2:550406/139‑1 (MQ=255) tCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAG‑GCC‑CCCCGAAAGCATCCCCTGACCGata < 2:361605/139‑1 (MQ=255) tACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAG‑GCC‑CCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCt > 2:825582/1‑139 (MQ=255) cacCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAG‑GCC‑CCCCGAAAGc < 2:936538/93‑1 (MQ=255) cacCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAG‑GCC‑CCCCGAAAGc > 1:936538/1‑93 (MQ=255) cacCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAG‑GCC‑CCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTgc > 2:1373144/1‑139 (MQ=255) cacCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAG‑GCC‑CCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTgc > 1:1291608/1‑139 (MQ=255) cacCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAG‑G‑c > 2:1173277/1‑82 (MQ=255) cacCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAG‑G‑c < 1:1173277/82‑1 (MQ=255) acCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAG‑GCC‑CCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTgcc > 2:1093357/1‑139 (MQ=255) ccTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGAC‑C‑CCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTgccg > 1:263408/1‑139 (MQ=255) cTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAG‑GCC‑CCCCGAAAGCATCCCCTGAcc > 2:1114866/1‑101 (MQ=255) cTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAG‑GCC‑CCCCGAAAGCATCCCCTGAcc < 1:1114866/101‑1 (MQ=255) tCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAG‑GCC‑CCCCGAAAGCATCCCCTGACCGATATAACca < 1:319521/109‑1 (MQ=255) tCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAG‑GCC‑CCCCGAAAGCATCCCCTGACCGATATAACca > 2:319521/1‑109 (MQ=255) cgccgcCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAG‑GCC‑CCCCGAAAGc > 1:19358/1‑87 (MQ=255) cgccgcCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAG‑GCC‑CCCCGAAAGc < 2:19358/87‑1 (MQ=255) ccgccATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAG‑GCC‑CCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCAccc > 2:1024121/1‑139 (MQ=255) cATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAG‑GCC‑CCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGAc > 2:1123212/1‑139 (MQ=255) cgcATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAG‑GCC‑CCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTc > 1:743453/1‑139 (MQ=255) gcATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAG‑GCC‑CCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTcc < 1:1024121/139‑1 (MQ=255) gcATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAG‑GCC‑CCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTcc > 2:32166/1‑139 (MQ=255) gcATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAG‑GCC‑CCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTcc < 2:261929/139‑1 (MQ=255) cATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAG‑GCC‑CCCCGAAAGCATCCCCTGAcc < 2:565949/75‑1 (MQ=255) cATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAG‑GCC‑CCCCGAAAGCATCCCCTGAcc > 1:565949/1‑75 (MQ=255) tCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAG‑G‑c‑cccccg > 2:1414988/1‑54 (MQ=255) tCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAG‑G‑c‑cccccg < 1:1414988/56‑3 (MQ=255) gATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAG‑GCC‑CCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAgg < 1:825582/139‑1 (MQ=255) tATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAG‑GCC‑CCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCAt > 1:382396/1‑139 (MQ=255) tATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAG‑GCC‑CCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCAt > 1:354576/1‑139 (MQ=255) tATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAG‑G‑c < 1:521525/40‑1 (MQ=255) tATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAG‑G‑c > 2:1366568/1‑40 (MQ=255) tATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAG‑G‑c > 2:521525/1‑40 (MQ=255) tATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAG‑G‑c < 1:1366568/40‑1 (MQ=255) gggCGTCGGTGAGGTGAAAATTTCGCCCGGACAG‑GCC‑CCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGc < 1:1093357/139‑1 (MQ=255) gggCGTCGGTGAGGTGAAAATTTCGCCCGGACAG‑GCC‑CCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGc < 2:354576/139‑1 (MQ=255) ggCGTCGGTGAGGTGAAAATTTCGCCCGGACAG‑GCC‑CCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCt < 2:263408/139‑1 (MQ=255) gCGTCGGTGAGGTGAAAATTTCGCCCGGACAG‑GCC‑CCCCGAAAGCATCCCCTGCCCGatat > 2:1087749/1‑61 (MQ=255) gCGTCGGTGAGGTGAAAATTTCGCCCGGACAG‑GCC‑CCCCGAAAGCATCCCCTGACCGatat < 1:1087749/61‑1 (MQ=255) gCGTCGGTGAGGTGAAAATTTCGCCCGGACAG‑GCC‑CCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTc > 2:600466/1‑139 (MQ=255) gCGTCGGTGAGGTGAAAATTTCGCCCGGACAG‑GCC‑CCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTc < 2:382396/139‑1 (MQ=255) gTCGGTGAGGTGAAAATTTCGCCCGGACAG‑GCC‑CCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCgg < 1:1373144/139‑1 (MQ=255) tgaggtgaAAATTTCGCCCGGACAG‑GCC‑CCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCGGGTGAc < 1:600466/139‑1 (MQ=255) ggtgaAAATTTCGCCCGGACAG‑GCC‑CCCCGAAAGCATCCCCTGACCGATATAAc < 1:117957/54‑1 (MQ=255) ggtgaAAATTTCGCCCGGACAG‑GCC‑CCCCGAAAGCATCCCCTGACCGATATAAc > 2:117957/1‑54 (MQ=255) ggtgaAAATTTCGCCCGGACAG‑GCC‑CCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCGGGTGACATa < 1:32166/139‑1 (MQ=255) tgaAAATTTCGCCCGGACAG‑GCC‑CCCCGAAAGCATCCCCTGAcc > 2:1016059/1‑44 (MQ=255) tgaAAATTTCGCCCGGACAG‑GCC‑CCCCGAAAGCATCCCCTGAcc < 1:1016059/44‑1 (MQ=255) tgaAAATTTCGCCCGGACAG‑GCC‑CCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCGGGTGACATaca < 2:743453/139‑1 (MQ=255) aaaTTTCGCCCGGACAG‑GCC‑CCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCGGGTGACATACACCg < 1:59663/139‑1 (MQ=255) aaaTTTCGCCCGGACAG‑GCC‑CCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCGGGTGACATACACCg < 2:1137983/139‑1 (MQ=255) cGGACAG‑GCC‑CCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCGGGTGACATACACCGGATCCTGATg < 2:1240715/139‑1 (MQ=255) | GGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAG‑G‑C‑CCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCGGGTGACATACACCGGATCCTGATG > NC_000913/1250488‑1250754 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |