Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 2,484,897 | (C)6→7 | coding (524/3594 nt) | evgS → | hybrid sensory histidine kinase in two‑component regulatory system with EvgA |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,484,891 | 1 | . | C | 100.0% | 224.0 / NA | 62 | Y173S (TAC→TCC) | evgS | hybrid sensory histidine kinase in two‑component regulatory system with EvgA |
Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (35/27); total (35/27) |
TTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑C‑CCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACC > NC_000913/2484754‑2485021 | ttCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑c < 2:880151/139‑1 (MQ=255) ttCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑c > 2:1407661/1‑139 (MQ=255) aatGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCCCCAGACGAGGTa < 2:891481/139‑1 (MQ=255) gACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑c > 1:1424471/1‑120 (MQ=255) gACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑c < 2:1424471/120‑1 (MQ=255) cATTGTTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCCCCAGACGAGGTAATtca > 1:49642/1‑139 (MQ=255) cATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCCCCAGACGAGGTAATtca > 2:1131610/1‑139 (MQ=255) ttGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCCCCAGACGAGGTAATtcatc > 2:1211637/1‑139 (MQ=255) gctgcAACCAAACCATTGATAATTCCCTTTCCGGGCCTGGTAACCACCCTTCGCGACTCAATTTGACCGCTAACCTCACCAAAACGAGTAAATATGGCTCGAGTACCACCTTA‑CGCCCCCAGACGAGGTAATTCatcaa > 2:1023297/1‑139 (MQ=255) gctgcAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCCCCAGACGAGGTAATTCatcaa > 2:250296/1‑139 (MQ=255) accaTTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCCCCAGACGAGGTAATTCATCAATCATTTCCaa > 2:529956/1‑138 (MQ=255) accaTTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCCCCAGACGAGGTAATTCATCAATCATTTCCaa < 1:529956/138‑1 (MQ=255) caTTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAg < 2:1340418/139‑1 (MQ=255) tACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTAt < 2:976056/139‑1 (MQ=255) tACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTAt > 1:976056/1‑139 (MQ=255) tACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTAt > 2:1289278/1‑139 (MQ=255) ccTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTAtct > 2:1209908/1‑139 (MQ=255) ttCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAAtt > 2:82193/1‑132 (MQ=255) ttCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAAtt < 1:82193/132‑1 (MQ=255) ttCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCtt > 1:1470683/1‑139 (MQ=255) ttCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCtt > 2:648443/1‑139 (MQ=255) ccGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCtttt > 2:482539/1‑139 (MQ=255) ccGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCtttt > 2:1148312/1‑139 (MQ=255) cGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTa < 1:1148312/139‑1 (MQ=255) cTGGAAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAAt > 2:439887/1‑139 (MQ=255) cctcccTTCACGACTCAATGCGACCGGTTACCGCTCCAACACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATc < 1:911987/136‑1 (MQ=255) accCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAg < 1:1407661/139‑1 (MQ=255) cccTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAgg > 2:285846/1‑139 (MQ=255) ttCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCAt > 2:55880/1‑139 (MQ=255) ttCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑c‑ccccccaga > 2:484999/1‑70 (MQ=255) ttCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑c‑ccccccaga < 1:484999/74‑5 (MQ=255) tCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTa > 1:651360/1‑113 (MQ=255) tCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTa < 2:651360/113‑1 (MQ=255) aaTGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGt > 2:321336/1‑139 (MQ=255) aaTGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATGACC‑CCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGt > 2:1139504/1‑139 (MQ=255) tGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTa < 2:244148/109‑1 (MQ=255) tGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTa > 1:244148/1‑109 (MQ=255) tGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGtct > 1:1421155/1‑139 (MQ=255) cTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTggg > 1:1350874/1‑139 (MQ=255) ttACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGc > 1:959999/1‑139 (MQ=255) ttACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGc < 1:321336/139‑1 (MQ=255) ccTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGcaca < 1:1209908/139‑1 (MQ=255) cACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTAt < 2:77198/86‑1 (MQ=255) cACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTAt > 1:77198/1‑86 (MQ=255) aCCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCGGTCTCAGCTGGGCACAATGa > 2:787563/1‑139 (MQ=255) aCCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGa < 1:1131610/139‑1 (MQ=255) aCCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGa < 2:49642/139‑1 (MQ=255) cAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAAt > 1:636874/1‑135 (MQ=255) cAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAAt < 2:636874/135‑1 (MQ=255) aaaCCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTAc < 2:1421155/139‑1 (MQ=255) aaCCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAAt > 2:662842/1‑93 (MQ=255) aaCCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAAt < 1:662842/93‑1 (MQ=255) gTAAATATTGCTCGGGTAGCAAATTACC‑CCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTAtt > 2:1030983/1‑139 (MQ=255) tAAATATTGCTCGGGTAGCAAATTACC‑CCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGc > 2:1411983/1‑65 (MQ=255) tAAATATTGCTCGGGTAGCAAATTACC‑CCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGc < 1:1411983/65‑1 (MQ=255) tAAATATTGCTCGGGTAGCAAATTACC‑CCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTg > 1:921914/1‑139 (MQ=255) ttGCTCGGGTAGCAAATTACC‑CCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGc < 1:908804/59‑1 (MQ=255) ttGCTCGGGTAGCAAATTACC‑CCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGc > 2:908804/1‑59 (MQ=255) ttGCTCGGGTAGCAAATTACC‑CCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGgtagta < 1:250296/139‑1 (MQ=255) ttGCTCGGGTAGCAAATTACC‑CCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGgtagta < 2:1470683/139‑1 (MQ=255) gCTCGGGTAGCAAATTACC‑CCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTAc < 2:622219/75‑1 (MQ=255) gCTCGGGTAGCAAATTACC‑CCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTAc > 1:622219/1‑75 (MQ=255) cTCGGGTAGCAAATTACC‑CCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGAt > 1:920728/1‑120 (MQ=255) cTCGGGTAGCAAATTACC‑CCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCACAGCTGGGCACAATGAt < 2:920728/120‑1 (MQ=255) tCGGGTAGCAAATTACC‑CCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAAcat > 1:57122/1‑139 (MQ=255) gTAGCAAATTACC‑CCCCCAGACGAGGTAATTCatcaa > 2:358894/1‑37 (MQ=255) gTAGCAAATTACC‑CCCCCAGACGAGGTAATTCatcaa < 1:358894/37‑1 (MQ=255) aGCAAATTACC‑CCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTAc < 1:399849/139‑1 (MQ=255) gCAAATTACC‑CCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACTATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTAcc < 2:1350874/139‑1 (MQ=255) | TTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑C‑CCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACC > NC_000913/2484754‑2485021 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |