Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,593,675	(C)5→6	coding (96/699 nt)	ypfH ←	palmitoyl‑CoA esterase activity, uncertain physiological substrate

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,593,670	1	.	C	100.0%	169.4 / NA	48	E34G (GAA→GGA)	ypfH	palmitoyl‑CoA esterase activity, uncertain physiological substrate
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (25/23);  total (25/23)

CACGCGCCTGGCGATTATCTTCCGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTT‑CCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGA  >  NC_000913/2593533‑2593788                                                                                                                                           |                                                                                                                       cacGCGCCTGGCGATTATCTTCCGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTT‑c                                                                                                                       >  2:1300704/1‑139 (MQ=255)       ccTGGCGATTATCTTCCGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTT‑cccccca                                                                                                                 >  2:561829/1‑137 (MQ=255)              aTTATCTTCCGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTCCCCCCATTGCCAc                                                                                                           <  2:425475/139‑1 (MQ=255)                tATCTTCCGTAATAGCCTGCACCGAAAACCACTGACGCCCCGCCGTATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTCCCCCCATTGCCACCg                                                                                                         <  1:1231171/139‑1 (MQ=255)                  tCTTCCGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTCCCCCCATTGCCACCggg                                                                                                       <  1:463866/139‑1 (MQ=255)                      ccGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTCCCCCCATTGCCACCGGGTTAt                                                                                                   >  1:221019/1‑139 (MQ=255)                                 tGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTCCCCCCATTGCCACCGGGTTATCCCCGACACCg                                                                                        >  1:1173183/1‑139 (MQ=255)                                 tGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTCCCCCCATTGCCACCGGGTTATCCCCGACACCg                                                                                        >  2:213450/1‑139 (MQ=255)                                 tGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTCCCCCCATTGCCACCGGGTTATCCCCGACACCg                                                                                        >  2:1196077/1‑139 (MQ=255)                                        aaaaCCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTCCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGaaaa                                                                                 <  1:1300704/139‑1 (MQ=255)                                        aaaaCCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTT‑cccccca                                                                                                                 >  1:1140845/1‑104 (MQ=255)                                        aaaaCCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTT‑cccccca                                                                                                                 <  2:1140845/106‑3 (MQ=255)                                        aaaaCCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTT‑ccc                                                                                                                     <  2:66394/102‑1 (MQ=255)                                        aaaaCCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTT‑ccc                                                                                                                     >  1:66394/1‑102 (MQ=255)                                                tGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTCCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTaac                                                                         >  2:60154/1‑139 (MQ=255)                                                  aCGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTCCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTaacaa                                                                       >  1:271410/1‑139 (MQ=255)                                                     ccccGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTCCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTg                                                                    >  2:279407/1‑139 (MQ=255)                                                                     cTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTCCCCCCATTGCCACCGGGTTATCCCCGacac                                                                                          <  1:1175057/101‑1 (MQ=255)                                                                     cTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTCCCCCCATTGCCACCGGGTTATCCCCGacac                                                                                          >  2:1175057/1‑101 (MQ=255)                                                                          cTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTAttt                                                                                                                         >  1:1333849/1‑65 (MQ=255)                                                                          cTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTAttt                                                                                                                         <  2:1333849/65‑1 (MQ=255)                                                                          cTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTCCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCt                                               >  1:444450/1‑139 (MQ=255)                                                                               cgcCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTCCCCCCATTGCCa                                                                                                            >  1:550584/1‑73 (MQ=255)                                                                               cgcCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTCCCCCCATTGCCa                                                                                                            <  2:550584/73‑1 (MQ=255)                                                                                  cACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTCCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGaacaa                                       >  2:391312/1‑139 (MQ=255)                                                                                            caccacCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTCCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCa                                                                              <  2:150380/90‑1 (MQ=255)                                                                                            caccacCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTCCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCa                                                                              >  1:150380/1‑90 (MQ=255)                                                                                            caccacCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTCCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTc                             >  2:724367/1‑139 (MQ=255)                                                                                                  caGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTT‑c                                                                                                                       >  1:1399621/1‑42 (MQ=255)                                                                                                  caGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTT‑c                                                                                                                       <  2:1399621/42‑1 (MQ=255)                                                                                                     cgcATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTCCCCCCATTGCCACCGGGtt                                                                                                     >  1:866185/1‑58 (MQ=39)                                                                                                     cgcATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTCCCCCCATTGCCACCGGGtt                                                                                                     <  2:866185/58‑1 (MQ=39)                                                                                                     cgcATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTCCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCAt                    >  2:1003679/1‑139 (MQ=255)                                                                                                     cgcATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTCCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCAt                    >  1:626629/1‑139 (MQ=255)                                                                                                     cgcATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTCCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCAt                    >  2:153261/1‑139 (MQ=255)                                                                                                       cATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTCCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATgg                  >  2:245294/1‑139 (MQ=255)                                                                                                       cATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTCCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATgg                  >  1:421462/1‑139 (MQ=255)                                                                                                         tCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTCCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCg                <  2:1173183/139‑1 (MQ=255)                                                                                                         tCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTCCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCg                <  2:421462/139‑1 (MQ=255)                                                                                                         tCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTCCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCg                <  1:213450/139‑1 (MQ=255)                                                                                                         tCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTCCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCg                <  2:626629/139‑1 (MQ=255)                                                                                                         tCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTCCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCg                <  1:60154/139‑1 (MQ=255)                                                                                                          cAGGAAACAGCGGTGCAAACCAGTTGCCTATTTCCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGa               <  2:444450/139‑1 (MQ=255)                                                                                                           aGGAAACAGCGGTGCAAACCAGTTGCCTATTTCCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGTAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGaa              <  1:1003679/139‑1 (MQ=255)                                                                                                           aGGAAACAGCGGTGCAAACCAGTTGCCTATTTCCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGaa              <  1:764573/139‑1 (MQ=255)                                                                                                           aGGAAACAGCGGTGCAAACCAGTTGCCTATTTCCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGaa              <  1:153261/139‑1 (MQ=255)                                                                                                                    cGGTGCAAACCAGTTGCCTATTTCCCCCCATTGCCACCGGGTTATCCCCGACACCGTgg                                                                                     >  2:321854/1‑59 (MQ=39)                                                                                                                    cGGTGCAAACCAGTTGCCTATTTCCCCCCATTGCCACCGGGTTATCCCCGACACCGTgg                                                                                     <  1:321854/59‑1 (MQ=39)                                                                                                                    cGGTGCAAACCAGTTGCCTATTTCCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTc           <  2:1381237/133‑1 (MQ=255)                                                                                                                    cGGTGCAAACCAGTTGCCTATTTCCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTc           >  1:1381237/1‑133 (MQ=255)                                                                                                                      gTGCAAACCAGTTGCCTATTTCCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTg   <  1:777658/139‑1 (MQ=255)                                                                                                                       tGCAAACCAGTTGCCTATTTCCCCCCATTGCCACCGGGTTGTCCCCGACACCGTGGAAAAGCAGTAACAACTGTTgcg                                                               >  2:436123/1‑78 (MQ=255)                                                                                                                       tGCAAACCAGTTGCCTATTTCCCCCCATTGCCACCGGGTTGTCCCCGACACCGTGGAAAAGCAGTAACAACTGTTgcg                                                               <  1:436123/78‑1 (MQ=255)                                                                                                                       tGCAAACCAGTTGCCTATTTCCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGa  >  2:572744/1‑139 (MQ=255)                                                                                                                                 ttGCCTATTTCCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGc                                                                               >  1:973920/1‑52 (MQ=255)                                                                                                                                 ttGCCTATTTCCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGc                                                                               <  2:973920/52‑1 (MQ=255)                                                                                                                                      tATTTCCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGaacaa                                       >  2:428542/1‑87 (MQ=255)                                                                                                                                      tATTTCCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGaacaa                                       <  1:428542/87‑1 (MQ=255)                                                                                                                                           |                                                                                                                       CACGCGCCTGGCGATTATCTTCCGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTT‑CCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGA  >  NC_000913/2593533‑2593788

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑