Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,767,445 (C)7→8 intergenic (+90/‑265) rnlB → / → yfjP CP4‑57 prophage, uncharacterized protein/CP4‑57 prophage, 50S ribosome‑binding GTPase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,767,4381.C83.7% 121.8 / 16.8 43intergenic (+83/‑272)rnlB/yfjPCP4‑57 prophage, uncharacterized protein/CP4‑57 prophage, 50S ribosome‑binding GTPase family protein
Reads supporting (aligned to +/- strand):  ref base . (6/1);  new base C (15/21);  total (21/22)
Fisher's exact test for biased strand distribution p-value = 4.59e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTCTGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATG  >  NC_000913/2767304‑2767570
                                                                                                                                        |                                                                                                                                      
ctctGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAAcccc                                                                                                                                  <  2:1052085/139‑1 (MQ=255)
 tctGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTaaacaac                                                                                                                                    >  1:1437324/1‑134 (MQ=255)
 tctGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAAccccc                                                                                                                                 >  1:1361550/1‑139 (MQ=255)
  ctGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAAcccc                                                                                                                                  >  1:578686/1‑137 (MQ=255)
  ctGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAAcccc                                                                                                                                  <  2:578686/137‑1 (MQ=255)
   tGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAAccccccc                                                                                                                               <  2:390945/139‑1 (MQ=255)
         gTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTcaca                                                                                                                          <  2:1361550/139‑1 (MQ=255)
         gTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTcaca                                                                                                                          <  1:668389/139‑1 (MQ=255)
            tcCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACg                                                                                                                       <  1:291607/139‑1 (MQ=255)
             cccGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGt                                                                                                                      >  1:522198/1‑139 (MQ=255)
              ccGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTc                                                                                                                     <  2:522198/139‑1 (MQ=255)
                 atgatgTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCACCttaacaaagtcatg                                                                                                                 >  1:146008/1‑126 (MQ=255)
                           gtaaGTCCTGTGCATTGATTTTTGAAACTCTTTTTTCTAAACGAACCAGTAGAGAAGTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAac                                                                                                        <  1:89838/137‑1 (MQ=255)
                           ccAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAac                                                                                                        <  1:1306764/139‑1 (MQ=255)
                                     tCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTccc                                                                                              <  1:115732/139‑1 (MQ=255)
                                               tttGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTcaca                                                                                                                          <  1:13524/101‑1 (MQ=255)
                                               tttGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTcaca                                                                                                                          >  2:13524/1‑101 (MQ=255)
                                                        cttcttTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTccc                                                                                              <  1:470331/120‑1 (MQ=255)
                                                        cttcttTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTccc                                                                                              >  2:470331/1‑120 (MQ=255)
                                                                        ccAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTaaa                                                           <  2:27857/139‑1 (MQ=255)
                                                                        ccAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTaaa                                                           <  2:146008/139‑1 (MQ=255)
                                                                          aGTATAGAAATCGCCCTTTTGGCATGAGATTAACTTGTCCAATATGATCAAATAGCAGTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACt                                                         <  1:868573/139‑1 (MQ=255)
                                                                                 aaTTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAAACCCCCCCACACAACGTCCTTCATATGGAAAAAGTATTCCCCTGTACACCCAAGACTAAATTCCCCCTCCTGAAAACTTATCaat                                                  >  1:27857/1‑139 (MQ=255)
                                                                                 aaTTCGCCCTTTTGGCATGATATTAACATGTCCAATATGATCAAATAGCATTAACCCCCCCCTTACAACGTTCGGCATAGGATATACGTTTTTCCCTGTACACCCAAGACAAAATTTAACCCTCTGCAAACTTCTCact                                                  >  1:877744/1‑137 (MQ=255)
                                                                                     cGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACACACCCCGAACAACGTCCTGCATAGGGAAACCGTTTTCCCCTATGCACCCACGACTCACTTTTACCCCCTCATAACTTCCCaatactc                                              >  1:300044/1‑136 (MQ=255)
                                                                                      gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAAACCCACCCTGACAACGGCCCGCATCGCGAACACCCTTTACCCTGTGCACACATGATTAAATTTCCCCCCCTTTAAAcctacaaaaaatgt                                             >  2:129958/1‑126 (MQ=255)
                                                                                      gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGt                                                                                                                      >  1:1141436/1‑66 (MQ=255)
                                                                                      gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGt                                                                                                                      <  2:1141436/66‑1 (MQ=255)
                                                                                      gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGa                                                                                                          <  2:865812/78‑1 (MQ=255)
                                                                                      gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGa                                                                                                          >  1:865812/1‑78 (MQ=255)
                                                                                      gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCAc                                                                                  <  1:750583/102‑1 (MQ=255)
                                                                                      gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCAc                                                                                  >  2:750583/1‑102 (MQ=255)
                                                                                      gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACACCCACCTTACAACGTATTCCATAGGGCACACGTTTTTCCATATGCCCCCACGACGAAATTTCCCCCCCTTTAAACTTCTCAATAATGt                                             >  2:678670/1‑139 (MQ=255)
                                                                                                              gTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTccc                                                                                              >  1:231463/1‑66 (MQ=255)
                                                                                                              gTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTccc                                                                                              <  2:231463/66‑1 (MQ=255)
                                                                                                               tCCAATATGATCAAATAGCATTAACCCCCCCCTCCCACCGACCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAACTTACTCCCCTTTAAACTTCTCACTAATGTCACCAATTTTCTGGAAATACTatta                    >  1:911068/1‑137 (MQ=255)
                                                                                                               tCCAATATGATCAAATAGCATTAACCCCCCCCTCACACCGTCCTGCATAGGGAACACGTTTTCCCCTGTGCAACCACGACTAAAGTTCCACCCCTTCAAACTTCTCAATAATGTCACCAATTTTCTGGAAACTCTatga                    >  2:1272307/1‑139 (MQ=255)
                                                                                                               tCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTTCACCCACGACTAAATTTCCCCCCCTTTAAACCTCTCAATAATTTCACCAATTTTCTGGAAATCCTatca                    >  1:462710/1‑137 (MQ=255)
                                                                                                               tCCAATATGATCAAATAGCATTAACCCACCCCGCACAACGTCCTGCATAGGGAACACGCTTATCACTGTGCACCACTGAAAAAATTTCACCCCCTTTAAACTTACCAATAATGACATCCATTTTCTGGAAAGCATatga                    >  1:161808/1‑139 (MQ=255)
                                                                                                                    tatGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTccc                                                                                              <  1:570321/60‑1 (MQ=255)
                                                                                                                    tatGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTccc                                                                                              >  2:570321/1‑60 (MQ=255)
                                                                                                                     atGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCATCAATTTTCTGGAAATCCTATGATGCCtt              <  2:911068/139‑1 (MQ=255)
                                                                                                                        aTCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACt                                                                              <  2:445433/72‑1 (MQ=255)
                                                                                                                        aTCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACt                                                                              >  1:445433/1‑72 (MQ=255)
                                                                                                                              tAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGAAACACGTCTTCCCCTGTGCACCCACGACTAAATATCTCCCCCTCTAAAGTTCTCAATTATTTCACCAATTTTCTGGAAATCTTATGACTCCTTTCCCGCTat     >  2:314989/1‑138 (MQ=255)
                                                                                                                               aGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCTCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTaaa    <  2:877744/139‑1 (MQ=255)
                                                                                                                               aGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTaaa    <  2:1437324/139‑1 (MQ=255)
                                                                                                                                 cATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACTACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAACTCCTATGATGCCTTTCCCGGTAAATg  >  2:707287/1‑139 (MQ=255)
                                                                                                                                   ttAACCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTccc                                                                     >  2:408319/1‑69 (MQ=255)
                                                                                                                                   ttAACCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTccc                                                                     <  1:408319/69‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                      
CTCTGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATG  >  NC_000913/2767304‑2767570

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: