| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,432,994 | (C)6→8 | coding (567/1125 nt) | smf ← | DNA recombination‑mediator A family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,432,988 | 1 | . | C | 92.2% | 156.3 / 6.1 | 51 | coding (573/1125 nt) | smf | DNA recombination‑mediator A family protein |
| Reads supporting (aligned to +/- strand): ref base . (2/2); new base C (23/24); total (25/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01 | |||||||||||
| * | NC_000913 | 3,432,988 | 2 | . | C | 83.7% | 136.6 / 20.0 | 51 | coding (573/1125 nt) | smf | DNA recombination‑mediator A family protein |
| Reads supporting (aligned to +/- strand): ref base . (4/4); new base C (20/21); total (25/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGA‑‑GCG‑‑CCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTACCTGTAAGGCTGCTTTATGCGCTACA > NC_000913/3432863‑3433111 || gAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGA‑‑GCG‑‑cccccccctgttc < 2:704395/139‑8 (MQ=255) aCGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGA‑‑GCGCCCCCCCCTGTTCAAGCAGAc < 2:693324/139‑1 (MQ=255) aGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGT‑‑GCGCCCCCCCCTGTTCAAGCAGACTGGCAg > 1:707017/1‑139 (MQ=255) tACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGA‑‑GCGCCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGAcggc > 2:898693/1‑139 (MQ=255) cacCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGA‑‑GCGCCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGAcggcgg > 1:1205005/1‑139 (MQ=255) tACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGA‑‑GCGCCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGa > 2:1195045/1‑139 (MQ=255) aCTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGA‑‑GCGCCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGaa < 2:1263687/139‑1 (MQ=255) cTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGA‑‑GCGCCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAAt < 2:659045/139‑1 (MQ=255) ttAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGA‑‑GCGCCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATg < 2:1205005/139‑1 (MQ=255) aCCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGA‑‑GCGCCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTAt > 2:655713/1‑139 (MQ=255) aCCACTGATAATGCGATTTCTCCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGTGCC‑‑CCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGa > 1:329529/1‑120 (MQ=255) aCCACTGATAATGCGATTTCATCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGA‑‑GCGCCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTAt > 2:997667/1‑139 (MQ=255) aCCACGGATAATGCGATTTCTTCGTAGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGTGCC‑‑CCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGa < 2:329529/120‑1 (MQ=255) aCTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGA‑‑GCGCCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTaa < 2:1285265/139‑1 (MQ=255) cTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGA‑‑GCGCTCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAg > 2:1244731/1‑139 (MQ=255) cTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGA‑‑GCGCCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAg > 1:232581/1‑139 (MQ=255) cTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGACGAGAGC‑‑GCC‑‑CCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTc > 1:421722/1‑139 (MQ=255) tGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGA‑‑GCGCCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTa < 2:12870/136‑1 (MQ=255) tGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGA‑‑GCGCCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTa > 1:12870/1‑136 (MQ=255) tGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGA‑‑GCGCCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGt > 2:835090/1‑139 (MQ=255) tGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGA‑‑GCGC‑CCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTc > 1:732933/1‑139 (MQ=255) tGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGA‑‑GCG‑‑cccccccctgt > 2:1208349/1‑80 (MQ=255) tGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGA‑‑GCG‑‑cccccccctgt < 1:1208349/85‑6 (MQ=255) gATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGA‑‑GCGCCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTc < 1:898693/139‑1 (MQ=255) gATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGACGAGAGC‑‑GCC‑‑CCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCa < 2:421722/139‑1 (MQ=255) tAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGA‑‑GCGCCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCa < 2:1469559/139‑1 (MQ=255) tAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGA‑‑GCGCCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCa < 1:997667/139‑1 (MQ=255) aTGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGA‑‑GCGCCCCCCCCTGTTCAAGCAGACTGGCAGCCAg > 2:400369/1‑100 (MQ=255) aTGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGA‑‑GCGCCCCCCCCTGTTCAAGCAGACTGGCAGCCAg < 1:400369/100‑1 (MQ=255) aTGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGA‑‑GCG‑‑cccccc > 2:1173982/1‑75 (MQ=255) aTGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGA‑‑GCG‑‑cccccc < 1:1173982/75‑1 (MQ=255) aTGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGA‑‑GCG‑‑cc > 1:779517/1‑71 (MQ=255) aTGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGA‑‑GCG‑‑cc < 2:779517/71‑1 (MQ=255) cGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGA‑‑GCGCCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTccc < 2:867928/139‑1 (MQ=255) cGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGA‑‑GCGCCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTccc < 1:655713/139‑1 (MQ=255) ggAGATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGA‑‑GCGCCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGTCGGCgggg > 1:947630/1‑102 (MQ=255) ggAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGA‑‑GCGCCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCgggg < 2:947630/102‑1 (MQ=255) ttGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGA‑‑GCGC‑CCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACg < 2:732933/139‑1 (MQ=255) tAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGA‑‑GCG‑‑cccccc < 1:394603/51‑1 (MQ=255) tAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGA‑‑GCG‑‑cccccc > 2:394603/1‑51 (MQ=255) cAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGA‑‑GCGCCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCAttt < 1:835090/139‑1 (MQ=255) ggggTGGAACATCGAGGGGAAATTCCGAGACGAGA‑‑GCGCCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTAcc > 1:847631/1‑139 (MQ=255) gggTGGAACATCGAGGGGAAATTCCGAGACGAGA‑‑GCGCCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGAt > 1:1079410/1‑88 (MQ=255) gggTGGAACATCGAGGGGAAATTCCGAGACGAGA‑‑GCGCCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGAt < 2:1079410/88‑1 (MQ=255) gggTGGAACATCGAGGGGAAATTCCGAGACGAGA‑‑GCG‑‑ccccccc > 1:484542/1‑43 (MQ=255) gggTGGAACATCGAGGGGAAATTCCGAGACGAGA‑‑GCG‑‑ccccccc < 2:484542/44‑2 (MQ=255) gTGGAACATCGAGGGGAAATTCCGAGACGAGA‑‑GCGCCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTACCTGt > 1:7281/1‑139 (MQ=255) ggAACATCGAGGGGAAATTCCGAGACGAGA‑‑GCGCCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTACCTGTaa > 2:255940/1‑139 (MQ=255) aaCATCGAGGGGAAATTCCGAGACGAGA‑‑GCGCCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCa > 2:231926/1‑99 (MQ=255) aaCATCGAGGGGAAATTCCGAGACGAGA‑‑GCGCCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCa < 1:231926/99‑1 (MQ=255) aaCATCGAGGGGAAATTCCGAGACGAGA‑‑GCGCCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTACCTGTAAgg < 1:255940/139‑1 (MQ=255) tCGAGGGGAAATTCCGAGACGAGA‑‑GCGCCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTc < 2:892856/93‑1 (MQ=255) tCGAGGGGAAATTCCGAGACGAGA‑‑GCGCCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTc > 1:892856/1‑93 (MQ=255) aGGGGAAATTCCGAGACGAGA‑‑GCGCTCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTACCTGTAAGGCTGCttt < 1:1244731/139‑1 (MQ=255) aGGGGAAATTCCGAGACGAGA‑‑GCGCCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTACCTGTAAGGCTGCttt > 1:1233833/1‑139 (MQ=255) ggAAATTCCGAGACGAGA‑‑GCGCCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTACCTGTAAGGCTGCTTTATg < 2:232581/139‑1 (MQ=255) aaaTTCCGAGACGAGA‑‑GCGC‑CCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCaa < 2:356200/94‑1 (MQ=255) aaaTTCCGAGACGAGA‑‑GCGC‑CCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCaa > 1:356200/1‑94 (MQ=255) aaTTCCGAGACGAGA‑‑GCGCCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTc < 1:701573/51‑1 (MQ=255) aaTTCCGAGACGAGA‑‑GCGCCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTc > 2:701573/1‑51 (MQ=255) aaTTCCGAGACGAGA‑‑GCGCCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTACCTGTAAGGCTGCTTTATgcgc > 1:980356/1‑139 (MQ=255) ccGAGACGAGA‑‑GCGCCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTACCTGTAAGGCTGCTTTATGCGCTaca < 1:253897/139‑1 (MQ=255) || GAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGA‑‑GCG‑‑CCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTACCTGTAAGGCTGCTTTATGCGCTACA > NC_000913/3432863‑3433111 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |