Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,518,9920C.25.7% 267.0 / 87.7 101pseudogene (272/504 nt)insB1IS1 transposase B,IS, phage, Tn, Transposon‑related functions, extrachromosomal, transposon related
Reads supporting (aligned to +/- strand):  ref base C (34/41);  new base . (12/14);  total (46/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 102 total aligned reads displayed.

ACAGTGGGGCTACGTCGGTGCTAAATCACGTCAGCGCTGGCTGTTTTACGCGTATGACAGGATACGGAGGACGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGC‑‑‑CTGCTGTCGGCCTTTGAGGTCGTGGTATAGATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTCAGCGCATTGAGCGACATAATCTG  >  NC_000913/4518855‑4519110
                                                                                                                                            |                                                                                                                      
aCAGTGGGGCTACGTCGGTGCTAAATCACGTCAGCGCTGGCTGTTTTACGCGTATGACAGGATACGGAGGACGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGC‑‑‑CTGCTGTCg                                                                                                                       <  2:788823/139‑1 (MQ=255)
          tACGTCGGTGCTAAATCACGTCAGCGCTGGCTGTTTTACGCGTATGACAGGATACGGAGGACGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGC‑‑‑CTGCTGTCGGCCTTTGAgg                                                                                                             <  2:1003857/139‑1 (MQ=255)
           aCGTCGGTGCTAAATCACGTCAGCGCTGGCTGTTTTACGCGTATGACAGGATACGGAGGACGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGC‑‑‑CTGCTGTCGGCCTTTGAGGt                                                                                                            <  1:1154887/139‑1 (MQ=255)
            cGTCGGTGCTAAATCACGTCAGCGCTGGCTGTTTTACGCGTATGACAGGATACGGAGGACGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGCTGTCGGCCTTTGAg                                                                                                              >  1:340647/1‑139 (MQ=21)
            cGTCGGTGCTAAATCACGTCAGCGCTGGCTGTTTTACGCGTATGACAGGATACGGAGGACGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGC‑‑‑CTGCTGTCGGCCTTTGAGGTc                                                                                                           <  2:866606/139‑1 (MQ=255)
            cGTCGGTGCTAAATCACGTCAGCGCTGGCTGTTTTACGCGTATGACAGGATACGGAGGACGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGC‑‑‑CTGCTGTCGGCCTTTGAGGTc                                                                                                           <  2:551794/139‑1 (MQ=255)
            cGTCGGTGCTAAATCACGTCAGCGCTGGCTGTTTTACGCGTATGACAGGATACGGAGGACGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGC‑‑‑CTGCTGTCGGCCTTTGAGGTc                                                                                                           <  2:1316226/139‑1 (MQ=255)
            cGTCGGTGCTAAATCACGTCAGCGCTGGCTGTTTTACGCGTATGACAGGATACGGAGGACGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGC‑‑‑CTGCTGTCGGCATTTGAGGTc                                                                                                           >  1:866606/1‑139 (MQ=255)
             gTCGGTGCTAAATCACGTCAGCGCTGGCTGTTTTACGCGTATGACAGGATACGGAGGACGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGC‑‑‑CTGCTGTCGGCCTTTGAGGTCg                                                                                                          <  2:1244781/139‑1 (MQ=255)
                ggTGCTAAATCACGTCAGCGCTGGCTGTTTTACGCGTATGACAGGATACGGAGGACGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGC‑‑‑CTGCTGTCGGCCTTTGAGGt                                                                                                            >  1:66578/1‑134 (MQ=255)
                ggTGCTAAATCACGTCAGCGCTGGCTGTTTTACGCGTATGACAGGATACGGAGGACGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGC‑‑‑CTGCTGTCGGCCTTTGAGGt                                                                                                            <  2:66578/134‑1 (MQ=255)
                 gTGCTAAATCACGTCAGCGCTGGCTGTTTTACGCGTATGACAGGATACGGAGGACGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGC‑‑‑CTGCTGTCGGCCTTTGAGGTc                                                                                                           >  2:1213683/1‑134 (MQ=255)
                 gTGCTAAATCACGTCAGCGCTGGCTGTTTTACGCGTATGACAGGATACGGAGGACGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGC‑‑‑CTGCTGTCGGCCTTTGAGGTc                                                                                                           <  1:1213683/134‑1 (MQ=255)
                  tGCTAAATCACGTCAGCGCTGGCTGTTTTACGCGTATGACAGGATACGGAGGACGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGC‑‑‑CTGCTGTCGGCCTTTGAGGTCGTGGta                                                                                                     <  2:488486/139‑1 (MQ=255)
                            cGTCAGCGCTGGCTGTTTTACGCGTATGACAGGATACGGAGGACGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGC‑‑‑CTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACgg                                                                                           >  2:230985/1‑139 (MQ=255)
                                 gcgcTGGCTGTTTTACGCGTATGACAGGATACGGAGGACGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGC‑‑‑CTGCTGTCGGCCTTTGAGGTc                                                                                                           <  2:500542/118‑1 (MQ=255)
                                 gcgcTGGCTGTTTTACGCGTATGACAGGATACGGAGGACGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGC‑‑‑CTGCTGTCGGCCTTTGAGGTc                                                                                                           >  1:500542/1‑118 (MQ=255)
                                  cgcTGGCTGTTTTACGCGTATGACAGGATACGGAGGACGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGC‑‑‑CTGCTGTCGGCCTTTGAGGt                                                                                                            <  2:1287394/116‑1 (MQ=255)
                                  cgcTGGCTGTTTTACGCGTATGACAGGATACGGAGGACGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGC‑‑‑CTGCTGTCGGCCTTTGAGGt                                                                                                            >  1:1287394/1‑116 (MQ=255)
                                     tggctgTTTTACGCGTATGACAGGCGCCGGATGACGGTTGTTGCGCACGTATTCGGTGAACGCACTATGGCGACGCTGGAGCGTCTTCTGAGC‑‑‑CTGCTGT‑GGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGcc                                                                                 <  1:80067/139‑1 (MQ=11)
                                     tggctgTTTTACGCGTATGACAGGATACGGAGGACGGTTGTGGCGCACGTCTTCGGTGAACCCATTCTGGCCCCCCTGGAGCGTCTTCTGAGC‑‑‑CTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGCCGGAtggctggc                                                                                  <  1:97856/139‑1 (MQ=21)
                                       gctgTTTTACGCGTATGACAGGATACGGAGGACGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGC‑‑‑CTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCg                                                                                <  1:809605/139‑1 (MQ=255)
                                        ctgTTTTACGCGTATGACAGGATACGGAGGACGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGC‑‑‑CTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGc                                                                               <  2:375653/139‑1 (MQ=255)
                                                  cgTATGACAGGCTCCGGAAGACGGTTGTTGCGCACGTATTCGGTGAACGCACTATGGCGACGCTGGAGCGTCTTCTGAGC‑‑‑CTGCTGT‑GGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCa                                                                    <  1:762653/139‑1 (MQ=11)
                                                    tATGACAGGATACGGAGGACGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGC‑‑‑CTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCAc                                                                   >  1:793629/1‑139 (MQ=255)
                                                     aTGACAGGATACGGAGGACGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGC‑‑‑CTGCTGTCGGCCTTTg                                                                                                                >  2:708565/1‑93 (MQ=255)
                                                     aTGACAGGATACGGAGGACGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGC‑‑‑CTGCTGTCGGCCTTTg                                                                                                                <  1:708565/93‑1 (MQ=255)
                                                              tccGGAAGACGGTTGTTGCGCACGTATTCGGTGAACGCACTATGGCGACGCTGGAGCGTCTTCTGAGC‑‑‑CTGCTGT‑GGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGa                                                        >  1:349925/3‑139 (MQ=11)
                                                                 ggaggaCGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGC‑‑‑CTGCTGTCGGCCTTTGa                                                                                                               <  1:1062068/82‑1 (MQ=255)
                                                                 ggaggaCGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGC‑‑‑CTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGAtggctgg                                                                                   >  2:762462/1‑110 (MQ=255)
                                                                 ggaggaCGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGC‑‑‑CTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGAtggctgg                                                                                   <  1:762462/110‑1 (MQ=255)
                                                                 ggaggaCGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGC‑‑‑CTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGaaa                                                      >  2:1218555/1‑139 (MQ=255)
                                                                 ggaggaCGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGC‑‑‑CTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGaaa                                                      >  1:563899/1‑139 (MQ=255)
                                                                    agaCGGTTGTTGCGCACGTATTCGGTGAACGCACCATGGCGACGCTGGAGCGTCTTCTGAGC‑‑‑CTGCTGT‑GGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTg                                                  <  1:758432/138‑1 (MQ=14)
                                                                      aCGGTTGTTGCGCACGTATTCGGTGAACGCACTATGGCGACGCTGGAGCGTCTTCTGAGC‑‑‑CTGCTGT‑GGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCa                                                <  2:375403/139‑1 (MQ=14)
                                                                      aCGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGC‑‑‑CTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGc                                                 >  1:848953/1‑139 (MQ=255)
                                                                       cGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGC‑‑‑CTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCg                                                                                <  1:17714/107‑1 (MQ=255)
                                                                       cGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGC‑‑‑CTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCg                                                                                >  2:17714/1‑107 (MQ=255)
                                                                        ggTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGC‑‑‑CTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCg                                                                                <  1:1357281/106‑1 (MQ=255)
                                                                        ggTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGC‑‑‑CTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCg                                                                                >  2:1357281/1‑106 (MQ=255)
                                                                               gcgcACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGC‑‑‑CTGCTGTCGGCCTTTGAGGTCGTGGta                                                                                                     <  2:1449499/78‑1 (MQ=255)
                                                                               gcgcACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGC‑‑‑CTGCTGTCGGCCTTTGAGGTCGTGGta                                                                                                     >  1:1449499/1‑78 (MQ=255)
                                                                                cgcACGTATTCGGTGAACGCACTATGGCGACGCTGGAGCGTCTTCTGAGC‑‑‑CTGCTGT‑GGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTAATCAGc                                      <  2:474480/139‑1 (MQ=14)
                                                                                 gcACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGC‑‑‑CTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGc                                      <  1:70828/139‑1 (MQ=255)
                                                                                  cACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGC‑‑‑CTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCa                                     >  2:1392170/1‑139 (MQ=255)
                                                                                  cACGTATTCGGTGAACGCACTATGGCGACGCTGGAGCGTCTTCTGAGC‑‑‑CTGCTGT‑GGCCTTTGAGGTCGTg                                                                                                        >  2:707615/1‑71 (MQ=11)
                                                                                  cACGTATTCGGTGAACGCACTATGGCGACGCTGGAGCGTCTTCTGAGC‑‑‑CTGCTGT‑GGCCTTTGAGGTCGTg                                                                                                        <  1:707615/71‑1 (MQ=11)
                                                                                    cGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGC‑‑‑CTGCTGTCGGCCTTTGAGGTCg                                                                                                          >  1:71888/1‑68 (MQ=255)
                                                                                    cGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGC‑‑‑CTGCTGTCGGCCTTTGAGGTCg                                                                                                          >  2:1103215/1‑68 (MQ=255)
                                                                                    cGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGC‑‑‑CTGCTGTCGGCCTTTGAGGTCg                                                                                                          <  2:71888/68‑1 (MQ=255)
                                                                                    cGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGC‑‑‑CTGCTGTCGGCCTTTGAGGTCg                                                                                                          <  1:1103215/68‑1 (MQ=255)
                                                                                    cGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGC‑‑‑CTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGc                                      <  1:1270166/136‑1 (MQ=255)
                                                                                    cGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGC‑‑‑CTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGc                                      >  2:1270166/1‑136 (MQ=255)
                                                                                        ttCGGTGAACGCACTATGGCGACGCTGGAGCGTCTTCTGAGC‑‑‑CTGCTGT‑GGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCa                                                                    <  2:472871/101‑1 (MQ=17)
                                                                                        ttCGGTGAACGCACTATGGCGACGCTGGAGCGTCTTCTGAGC‑‑‑CTGCTGT‑GGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCa                                                                    <  2:690875/101‑1 (MQ=17)
                                                                                        ttCGGTGAACGCACTATGGCGACGCTGGAGCGTCTTCTGAGC‑‑‑CTGCTGT‑GGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCa                                                                    >  1:472871/1‑101 (MQ=17)
                                                                                        ttCGGTGAACGCACTATGGCGACGCTGGAGCGTCTTCTGAGC‑‑‑CTGCTGT‑GGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCa                                                                    >  1:690875/1‑101 (MQ=17)
                                                                                               aaCGCACTCTGGCCACACTGGAGCGTCTTCTGAGC‑‑‑CTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGa                                                        <  1:10295/107‑1 (MQ=255)
                                                                                               aaCGCACTCTGGCCACACTGGAGCGTCTTCTGAGC‑‑‑CTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGa                                                        >  2:10295/1‑107 (MQ=255)
                                                                                                 cGCACTCTGGCCACACTGGAGCGTCTTCTGAGC‑‑‑CTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCaa                                    <  1:1150419/125‑1 (MQ=255)
                                                                                                 cGCACTCTGGCCACACTGGAGCGTCTTCTGAGC‑‑‑CTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCaa                                    >  2:1150419/1‑125 (MQ=255)
                                                                                                 cGCACTATGGCGACGCTGGAGCGTCTTCTGAGC‑‑‑CTGCTGT‑GGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTAATCAGCAAGCGATATACGCAgcg                     >  2:1447187/1‑139 (MQ=11)
                                                                                                  gCACTCTGGCCACACTGGAGCGTCTTCTGAGC‑‑‑CTGTTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTCAgcg                     <  2:1055418/139‑1 (MQ=255)
                                                                                                       atGGCGACGCTGGAGCGTCTTCTGAGC‑‑‑CTGCTGT‑GGCCTTTGAGGTCGTGGTATATATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTAATCAGCAAGCGATATACGCAGCGAATTGa               <  2:780090/138‑1 (MQ=11)
                                                                                                         ggCCACACTGGAGCGTCTTCTGAGC‑‑‑CTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGcc                                                                                 <  2:1243938/72‑1 (MQ=255)
                                                                                                         ggCCACACTGGAGCGTCTTCTGAGC‑‑‑CTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGcc                                                                                 >  1:1243938/1‑72 (MQ=255)
                                                                                                         ggCCACACTGGAGCGTCTTCTGAGC‑‑‑CTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTCAGCGCATTGAg              <  2:15897/139‑1 (MQ=255)
                                                                                                         ggCCACACTGGAGCGTCTTCTGAGC‑‑‑CTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTCAGCGCATTGAg              <  1:1392170/139‑1 (MQ=255)
                                                                                                             acgcTGGAGCGTCTTCTGAGC‑‑‑CTGCTGT‑GGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTAATCAGCAAGCGATATACGCAGCGAATTGAGCggta         <  2:1194532/136‑4 (MQ=11)
                                                                                                              cgcTGGAGCGTCTTCTGAGC‑‑‑CTGCTGT‑GGCCTTTGAgg                                                                                                             <  2:1348202/36‑1 (MQ=25)
                                                                                                              cgcTGGAGCGTCTTCTGAGC‑‑‑CTGCTGT‑GGCCTTTGAgg                                                                                                             >  1:1348202/3‑38 (MQ=25)
                                                                                                              cgcTGGAGCGTCTTCTGAGC‑‑‑CTGCTGT‑GGCCTTTGAGGt                                                                                                            <  1:346171/37‑1 (MQ=37)
                                                                                                              cgcTGGAGCGTCTTCTGAGC‑‑‑CTGCTGT‑GGCCTTTGAGGt                                                                                                            >  2:346171/3‑39 (MQ=37)
                                                                                                              cgcTGGAGCGTCTTCTGAGC‑‑‑CTGCTGT‑GGCCTTTGAGGTCGTGGtataaa                                                                                                 <  2:709854/48‑3 (MQ=38)
                                                                                                              cgcTGGAGCGTCTTCTGAGC‑‑‑CTGCTGT‑GGCCTTTGAGGTCGTGGtataaa                                                                                                 >  1:709854/3‑48 (MQ=38)
                                                                                                              cacTGGAGCGTCTTCTGAGC‑‑‑CTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCg                                 <  1:1417102/115‑1 (MQ=255)
                                                                                                              cacTGGAGCGTCTTCTGAGC‑‑‑CTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCg                                 >  2:1417102/1‑115 (MQ=255)
                                                                                                                cTGGAGCGTCTTCTGAGC‑‑‑CTGCTGTCGGCCTTTGAGGTCg                                                                                                          <  1:1132833/40‑1 (MQ=255)
                                                                                                                cTGGAGCGTCTTCTGAGC‑‑‑CTGCTGTCGGCCTTTGAGGTCg                                                                                                          >  2:1132833/1‑40 (MQ=255)
                                                                                                                  ggAGCGTCTTCTGAGC‑‑‑CTGCTGT‑GGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTAATCAGCAAGCGATATACGCAGCGAATTGAGCggtataacc    >  2:391415/1‑131 (MQ=11)
                                                                                                                  ggAGCGTCTTCTGAGC‑‑‑CTGCTGT‑GGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTAATCAGCAAGCGATATACGCAGCGAATTGAGCGGTATaacc    >  2:130611/1‑137 (MQ=11)
                                                                                                                    aGCGTCTTCTGAGC‑‑‑CTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCg                                                                                >  1:330290/1‑62 (MQ=255)
                                                                                                                    aGCGTCTTCTGAGC‑‑‑CTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCg                                                                                <  2:330290/62‑1 (MQ=255)
                                                                                                                    aGCGTCTTCTGAGC‑‑‑CTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTCAGCGCATTGAGCGACATaatct   >  2:1373476/1‑139 (MQ=255)
                                                                                                                    aGCGTCTTCTGAGC‑‑‑CTGCTGT‑GGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTAATCAGCAAGCGATATACGCAGCGAATTGAGCGGTATaacctg  >  2:469154/1‑139 (MQ=11)
                                                                                                                        tcttctGAGC‑‑‑CTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGt                                <  2:1078235/106‑1 (MQ=255)
                                                                                                                        tcttctGAGC‑‑‑CTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGt                                >  1:1078235/1‑106 (MQ=255)
                                                                                                                         cttctGAGC‑‑‑CTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCg                                                                                <  1:728008/57‑1 (MQ=255)
                                                                                                                         cttctGAGC‑‑‑CTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCg                                                                                >  2:728008/1‑57 (MQ=255)
                                                                                                                         cttctGAGC‑‑‑CTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTg                                                  <  1:413894/87‑1 (MQ=255)
                                                                                                                         cttctGAGC‑‑‑CTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTg                                                  >  2:413894/1‑87 (MQ=255)
                                                                                                                           tctGAGC‑‑‑CTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCg                                                                                <  2:62476/55‑1 (MQ=255)
                                                                                                                           tctGAGC‑‑‑CTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCg                                                                                >  1:62476/1‑55 (MQ=255)
                                                                                                                              gAGC‑‑‑CTGCTGT‑GGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTAATCAGCAAGCGATATACGCAGCGAATTGAGc             >  1:990696/1‑118 (MQ=14)
                                                                                                                                      tgctgTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGc                                  <  2:663640/93‑1 (MQ=255)
                                                                                                                                      tgctgTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGc                                  >  1:663640/1‑93 (MQ=255)
                                                                                                                                      tgctgTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTCAGCGCATTGAGCGa           <  1:1185462/116‑1 (MQ=255)
                                                                                                                                      tgctgTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTCAGCGCATTGAGCGa           >  2:1185462/1‑116 (MQ=255)
                                                                                                                                        ctgTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTCAGCGCATTGa               >  1:1161168/1‑110 (MQ=255)
                                                                                                                                        ctgTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTCAGCGCATTGa               <  2:1161168/110‑1 (MQ=255)
                                                                                                                                            |                                                                                                                      
ACAGTGGGGCTACGTCGGTGCTAAATCACGTCAGCGCTGGCTGTTTTACGCGTATGACAGGATACGGAGGACGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGC‑‑‑CTGCTGTCGGCCTTTGAGGTCGTGGTATAGATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTCAGCGCATTGAGCGACATAATCTG  >  NC_000913/4518855‑4519110

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑