Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	484,106	C→T	A100T (GCG→ACG)	acrB ←	multidrug efflux system protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	484,106	0	C	T	100.0%	237.1 / NA	73	A100T (GCG→ACG)	acrB	multidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/44);  total (29/44)

TTGATAACGCCGACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCA  >  NC_000913/483973‑484234                                                                                                                                      |                                                                                                                                 ttGATAACGCCGACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGTATCAg                                                                                                                             >  2:1295947/1‑139 (MQ=255)       aCGCCGACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGTATCAGTACCAg                                                                                                                       <  2:533275/139‑1 (MQ=255)        cGCCGACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGTATCAGTACCa                                                                                                                        >  2:499217/1‑137 (MQ=255)        cGCCGACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGTATCAGTACCa                                                                                                                        <  1:499217/137‑1 (MQ=255)         gCCGACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGTATCAGTACCAGAc                                                                                                                     >  2:808391/1‑139 (MQ=255)         gCCGACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGTATCAGTACCAGAc                                                                                                                     >  1:656906/1‑139 (MQ=255)         gCCGACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGTATCAGTACCAGAc                                                                                                                     >  2:110999/1‑139 (MQ=255)            gACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGTATCAGTACCAGACTCa                                                                                                                  <  1:1429818/139‑1 (MQ=255)            gACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACAGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGTATCAGTACCAGACTCa                                                                                                                  <  1:393588/139‑1 (MQ=255)                   aTCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGTATCAGTACCAGACTCAAAGGTCa                                                                                                           <  1:676139/139‑1 (MQ=255)                   aTCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCAATATCCGTATCAGTACCAGACTCAAAGGTCa                                                                                                           <  1:1247465/139‑1 (MQ=255)                     cAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCAGCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGTATCAGTACCAGACTCAAAGGTCAgg                                                                                                         <  2:357623/139‑1 (MQ=255)                        gAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGTATCa                                                                                                                              <  2:1140417/115‑1 (MQ=255)                        gAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGTATCa                                                                                                                              >  1:1140417/1‑115 (MQ=255)                            ctgctgGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGTATCAGTACCAGACTCAAAGGTCAGGGTGATCt                                                                                                  >  2:376002/1‑139 (MQ=255)                              gctgGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGTATCAGTACCAGACTCAAAGGTCAGGGTGATCTGc                                                                                                <  1:710338/139‑1 (MQ=255)                               ctgGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGTATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCa                                                                                               >  2:1235270/1‑139 (MQ=255)                               ctgGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGTATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCa                                                                                               <  1:1083911/139‑1 (MQ=255)                                  gatgatTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGTATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACgg                                                                                            >  1:1083471/1‑139 (MQ=255)                                   atgatTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGTATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGt                                                                                           <  1:44657/139‑1 (MQ=255)                                      atTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGTAt                                                                                                                                <  1:1080189/99‑1 (MQ=255)                                      atTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGTAt                                                                                                                                >  2:1080189/1‑99 (MQ=255)                                       tttCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGTATCAGTACCAGACTCa                                                                                                                  >  1:248032/1‑112 (MQ=255)                                       tttCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGTATCAGTACCAGACTCa                                                                                                                  <  2:248032/112‑1 (MQ=255)                                        ttCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGTATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCg                                                                                      <  2:1083471/139‑1 (MQ=255)                                             aaCGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGTATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAg                                                                                 >  2:769005/1‑139 (MQ=255)                                              aCGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGTATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGt                                                                                <  1:1296830/139‑1 (MQ=255)                                                gCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGTATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCa                                                                              <  1:1295947/139‑1 (MQ=255)                                                 cTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGTATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCAc                                                                             >  1:177635/1‑139 (MQ=255)                                                 cTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGTATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCAc                                                                             <  2:266872/139‑1 (MQ=255)                                                 cTCACCCCTTGCTGCTGAACGTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAAGCTGCGCGATATACGTATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCAc                                                                             <  2:1067959/139‑1 (MQ=255)                                                    aCCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGTATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCa                                                                                               >  1:1111693/1‑118 (MQ=255)                                                    aCCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGTATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCa                                                                                               <  2:1111693/118‑1 (MQ=255)                                                       ccTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGTATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTag                                                                       <  1:397844/139‑1 (MQ=255)                                                         ttGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGTATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTagag                                                                     <  2:571115/139‑1 (MQ=255)                                                         ttGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGTATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTagag                                                                     <  2:1100228/139‑1 (MQ=255)                                                         ttGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGTATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTagag                                                                     >  1:13655/1‑139 (MQ=255)                                                         ttGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGTATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTagag                                                                     <  1:376002/139‑1 (MQ=255)                                                         ttGCTGCTGAACTTCTTGCAGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGTATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTagag                                                                     >  1:1194112/1‑139 (MQ=255)                                                          tgctgcTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGTATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAgg                                                                    <  2:1142780/139‑1 (MQ=255)                                                          tgctgcTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGTATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAgg                                                                    <  2:13655/139‑1 (MQ=255)                                                           gctgctGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGTATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGa                                                                   >  2:710912/1‑139 (MQ=255)                                                            ctgctgAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGTATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGAc                                                                  <  1:1171711/139‑1 (MQ=255)                                                             tgctgAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGTATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACa                                                                 <  1:917664/139‑1 (MQ=255)                                                             tgctgAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGTATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACa                                                                 <  2:1168031/139‑1 (MQ=255)                                                               ctgAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGTATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATg                                                               >  1:1298680/1‑139 (MQ=255)                                                                tgAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGTATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATg                                                               >  1:698219/1‑138 (MQ=255)                                                                tgAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGTATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATg                                                               <  2:698219/138‑1 (MQ=255)                                                                tgAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGTATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGt                                                              >  1:333615/1‑139 (MQ=255)                                                                 gAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGTATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTa                                                             <  2:1298680/139‑1 (MQ=255)                                                                   aCTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGTATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACa                                                           <  1:1235270/139‑1 (MQ=255)                                                                   aCTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGTATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACa                                                           <  2:5137/139‑1 (MQ=255)                                                                   aCTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGTATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACa                                                           <  2:177635/139‑1 (MQ=255)                                                                    cttcttGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGTATCAGTACCAGACTCa                                                                                                                  <  2:1287626/83‑1 (MQ=255)                                                                    cttcttGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGTATCAGTACCAGACTCa                                                                                                                  >  1:1287626/1‑83 (MQ=255)                                                                            ggCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGTATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTAt                                                  >  2:32500/1‑139 (MQ=255)                                                                              cagcaACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGTATCAGTAc                                                                                                                          >  1:1418992/1‑65 (MQ=255)                                                                              cagcaACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGTATCAGTAc                                                                                                                          <  2:1418992/65‑1 (MQ=255)                                                                               agcaACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGTATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGa                                               <  1:769005/139‑1 (MQ=255)                                                                                 caACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGTATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAg                                                       <  1:1292233/129‑1 (MQ=255)                                                                                 caACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGTATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAg                                                       >  2:696771/1‑129 (MQ=255)                                                                                 caACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGTATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAg                                                       <  1:696771/129‑1 (MQ=255)                                                                                 caACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGTATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAg                                                       >  2:1292233/1‑129 (MQ=255)                                                                                  aaCGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGTATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGATAc                                            <  2:656906/139‑1 (MQ=255)                                                                                                        tGTTCTGCACCTGAACCTGCGCGATATCCGTATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAAc                      <  2:333615/139‑1 (MQ=255)                                                                                                             tGCACCTGAACCTGCGCGATATCCGTATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCa                                                                                               >  2:1128200/1‑61 (MQ=255)                                                                                                             tGCACCTGAACCTGCGCGATATCCGTATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCa                                                                                               <  1:1128200/61‑1 (MQ=255)                                                                                                                aCCTGAACCTGCGCGATATCCGTATCAGTACCAGACTCAAAGGTCAgg                                                                                                         >  2:58110/1‑48 (MQ=255)                                                                                                                aCCTGAACCTGCGCGATATCCGTATCAGTACCAGACTCAAAGGTCAgg                                                                                                         <  1:58110/48‑1 (MQ=255)                                                                                                                     aaCCTGCGCGATATCCGTATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCgtg         >  2:363698/1‑139 (MQ=255)                                                                                                                         tGCGCGATATCCGTATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGAt                         >  2:537082/1‑119 (MQ=255)                                                                                                                         tGCGCGATATCCGTATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGAt                         <  1:537082/119‑1 (MQ=255)                                                                                                                            gcgATATCCGTATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCa  <  1:808391/139‑1 (MQ=255)                                                                                                                                      |                                                                                                                                 TTGATAACGCCGACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCA  >  NC_000913/483973‑484234

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑