Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	515,808	C→T	intergenic (‑35/‑111)	qmcA ← / → fetA	PHB domain membrane‑anchored putative protease/iron export ABC transporter ATPase, peroxide resistance protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	515,808	0	C	T	100.0%	181.3 / NA	56	intergenic (‑35/‑111)	qmcA/fetA	PHB domain membrane‑anchored putative protease/iron export ABC transporter ATPase, peroxide resistance protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (30/26);  total (30/26)

TCCACTGATAGCCCTGCGGTACGATTTTGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAGAAAAAGGGAACAGCGATGCAGGAAAATAGTCCTTTGC  >  NC_000913/515686‑515940                                                                                                                           |                                                                                                                                     tCCACTGATAGCCCTGCGTTACGATTTTGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGTAAAATTGCTGCTTGAt                                                                                                                      >  2:967649/1‑139 (MQ=255) tCCACTGATAGCCCTGCGGTACGATTTTGACAACCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGTAAAATTGCTGCTTGAt                                                                                                                      >  1:872655/1‑139 (MQ=255)     cTGATAGCCCTGCGGTACGATTTTGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGTAAAATTGCTGCTTGATTaaa                                                                                                                  <  2:1221899/139‑1 (MQ=255)     cTGATAGCCCTGCGGTACGATTTTGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGTAAAATTGCTGCTTGATTaaa                                                                                                                  <  2:514756/139‑1 (MQ=255)     cTGATAGCCCTGCGGTACGATTTTGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGTAAAATTGCTGCTTGATTaaa                                                                                                                  <  2:575792/139‑1 (MQ=255)        aTAGCCCTGCGGTACGATTTTGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGTAAAATTGCTGCTTGATTAAACaa                                                                                                               >  1:1132948/1‑139 (MQ=255)            cccTGCGGTACGATTTTGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGTAAAATTGCTGCTTGATTAAACAAATta                                                                                                           <  1:920045/139‑1 (MQ=255)                gCGGTACGATTTTGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGTAAAATttctg                                                                                                                            >  2:818161/1‑118 (MQ=255)                gCGGTACGATTTTGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGTAAAATtgctg                                                                                                                            <  1:818161/118‑1 (MQ=255)                gCGGTACGATTTTGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGTAAAATTGCTGCTTGATTAAACAAATTATAcc                                                                                                       >  1:219020/1‑139 (MQ=255)                gCGGTACGATTTTGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGTAAAATTGCTGCTTGATTAAACAAATTATAc                                                                                                        <  2:229361/138‑1 (MQ=255)                gCGGTACGATTTTGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGTAAAATTGCTGCTTGATTAAACAAATTATAc                                                                                                        >  1:229361/1‑138 (MQ=255)                    tACGATTTTGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAACCCTCCTGTTGTACCGTCCATAATCAGTAAAATTGCTGCTTGATTAAACAAATTATACCTGAt                                                                                                   <  1:959667/139‑1 (MQ=255)                      cGATTTTGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGTAAAATTGCTGCTTGATTAAACAAATTATACCTGATTa                                                                                                 <  1:1135071/139‑1 (MQ=255)                      cGATTTTGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGTAAAATTGCTGCTTGATTAAACAAATTATACCTGATTa                                                                                                 <  2:1132948/139‑1 (MQ=255)                           ttGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGTAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGaa                                                                                            >  1:330252/1‑139 (MQ=255)                                 cccGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGTAAAATTGCTGCTTGATTAAACAAATtata                                                                                                         >  2:846719/1‑120 (MQ=255)                                 cccGCGCCCACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGTAAAATTGCTGCTTGATTAAACAAATtata                                                                                                         <  1:846719/120‑1 (MQ=255)                                  ccGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATACTCAGTAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGt                                                                                     <  1:967649/139‑1 (MQ=255)                                   cgcgCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGTAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGtt                                                                                    >  1:381801/1‑139 (MQ=255)                                       ccGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGTAAAATTGCTGCTTGATTaaa                                                                                                                  >  1:451186/1‑105 (MQ=255)                                       ccGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGTAAAATTGCTGCTTGATTaaa                                                                                                                  <  2:451186/105‑1 (MQ=255)                                         gACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGTAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCt                                                                              <  2:219020/139‑1 (MQ=255)                                                aCCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGTAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCt                                                                       >  1:1152224/1‑139 (MQ=255)                                                  cAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGTAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGc                                                                     <  2:1152224/139‑1 (MQ=255)                                                    gcgcGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGTAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGa                                                                   >  1:724829/1‑139 (MQ=255)                                                     cgcgACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGTAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGaa                                                                  >  2:1442528/1‑139 (MQ=255)                                                     cgcgACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGTAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGa                                                                   <  1:1446488/138‑1 (MQ=255)                                                     cgcgACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGTAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGa                                                                   >  2:1446488/1‑138 (MQ=255)                                                      gcgACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGTAAAATTGCTGCTTGAt                                                                                                                      <  1:707097/86‑1 (MQ=255)                                                      gcgACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGTAAAATTGCTGCTTGAt                                                                                                                      >  2:707097/1‑86 (MQ=255)                                                          cAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGTAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATa                                                             >  2:69818/1‑139 (MQ=255)                                                          cAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGTAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATa                                                             >  1:363920/1‑139 (MQ=255)                                                                                    aGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGTAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTc                                                                                   >  1:652356/1‑91 (MQ=255)                                                                                    aGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGTAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTc                                                                                   <  2:652356/91‑1 (MQ=255)                                                                                        tCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGTAAAAtt                                                                                                                                >  2:1025173/1‑42 (MQ=255)                                                                                        tCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGTAAAAtt                                                                                                                                <  1:1025173/42‑1 (MQ=255)                                                                                        tCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGTAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTAATCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAGAAAAAggg                               >  1:1178385/1‑139 (MQ=255)                                                                                         cGGAAAAACCTCCTGTTGTACCGTCCATAATCAGTAAAATTGCTGCTTGATTAAACAAATTATACCTGAt                                                                                                   <  1:1030407/70‑1 (MQ=255)                                                                                         cGGAAAAACCTCCTGTTGTACCGTCCATAATCAGTAAAATTGCTGCTTGATTAAACAAATTATACCTGAt                                                                                                   >  2:1030407/1‑70 (MQ=255)                                                                                         cGGAAAAACCTCCTGTTGTACCGTCCATAATCAGTAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAGAAAAAGGGa                              >  2:1197351/1‑139 (MQ=255)                                                                                          ggAAAAACCTCCTGTTGTACCGTCCATAATCAGTAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGa                                                                   >  2:283428/1‑101 (MQ=255)                                                                                          ggAAAAACCTCCTGTTGTACCGTCCATAATCAGTAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGa                                                                   <  1:283428/101‑1 (MQ=255)                                                                                           gAAAAACCTCCTGTTGTACCGTCCATAATCAGTAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGc                                                                     >  2:1039118/1‑98 (MQ=255)                                                                                           gAAAAACCTCCTGTTGTACCGTCCATAATCAGTAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGc                                                                     <  1:1039118/98‑1 (MQ=255)                                                                                           gAAAAACCTCCTGTTGTACCGTCCATAATCAGTAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAGAAAAAGGGAAc                            >  2:113657/1‑139 (MQ=255)                                                                                                aCCTCCTGTTGTACCGTCCATAATCAGTAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAGAAAAAGGGAACAGCGa                       >  2:683163/1‑139 (MQ=255)                                                                                                aCCTCCTGTTGTACCGTCCATAATCAGTAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAGAAAAAGGGAACAGCGa                       <  1:931854/139‑1 (MQ=255)                                                                                                    cctGTTGTACCGTCCATAATCAGTAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAGAAAAAGGGAACAGCGATGCa                   <  1:113657/139‑1 (MQ=255)                                                                                                           tACCGTCCATAATCAGTAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAGAAAAAGGGAACAGCGATGCAGGAAAAt            <  2:381801/139‑1 (MQ=255)                                                                                                                tCCATAATCAGTAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAGAAAAAGGGAACAGCGATGCAGGAAAATAGTcc       >  1:161345/1‑139 (MQ=255)                                                                                                                tCCATAATCAGTAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAGAAAAAGGGAACAGCGATGCAGGAAAATAGTcc       >  2:946044/1‑139 (MQ=255)                                                                                                                  cATAATCAGTAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAGAAAAAGGGAACAGCGATGCAGGAAAATAGTCCtt     >  1:960221/1‑139 (MQ=255)                                                                                                                     aaTCAGTAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAGAAAAAGGGAACAGCGa                       >  2:959949/1‑118 (MQ=255)                                                                                                                     aaTCAGTAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAGAAAAAGGGAACAGCGa                       <  1:959949/118‑1 (MQ=255)                                                                                                                     aaTCAGTAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAGAAAAAGGGAACAGCGATGCAGGAAAATAGTCCTTTGc  <  2:724829/139‑1 (MQ=255)                                                                                                                           |                                                                                                                                     TCCACTGATAGCCCTGCGGTACGATTTTGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAGAAAAAGGGAACAGCGATGCAGGAAAATAGTCCTTTGC  >  NC_000913/515686‑515940

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑