Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	524,158	C→T	L300L (CTG→TTG)	rhsD →	Rhs protein with DUF4329 family putative toxin domain, putative neighboring cell growth inhibitor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	524,158	0	C	T	100.0%	163.3 / NA	52	L300L (CTG→TTG)	rhsD	Rhs protein with DUF4329 family putative toxin domain, putative neighboring cell growth inhibitor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (27/25);  total (27/25)

CGGAAGAGGCCCGCACCTCTTCGCTATCTTCTTCTGACAGTTCCCGCCCTCTCTCAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCG‑‑GTGTGGCTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACGGAAGCCGGTGAACTGCTGGCGGTATATGACCGCAGCAATACGCAGGTGCGCGCT  >  NC_000913/524027‑524277                                                                                                                                      |                                                                                                                        cGGAAGAGGCCCGCACCTCTTCGCTATCTTCTTCTGACAGTTTCCGCCCTCTCTCAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCG‑‑GTGTGGTTGATGCa                                                                                                                  >  2:383250/1‑139 (MQ=255)              cACCTCTTCGCTATCTTCTTCTGACAGTTCCCGCCCTCTCTCAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCG‑‑GTGTGGTTGATGCACGACCCGGCATAc                                                                                                     >  1:1316950/1‑139 (MQ=255)                                 tctGACAGTTCCCGCCCTCTCTCAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCG‑‑GTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTg                                                                                  <  1:1012053/139‑1 (MQ=255)                                    gACAGTTCCCGCCCTCTCTCAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCG‑‑GTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTgcgc                                                                               <  1:241693/139‑1 (MQ=255)                                     aCAGTTCCCGCCCTCTCTCAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCG‑‑GTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGcc                                                                              >  2:1305807/1‑139 (MQ=255)                                       aGTTCCCGCCCTCTCTCAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCG‑‑GTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCAc                                                                            >  1:468782/1‑139 (MQ=255)                                          tCCCGCCCTCTCTCAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCG‑‑GTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTgg                                                                         <  1:1305807/139‑1 (MQ=255)                                          tCCCGCCCTCTCTCAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCG‑‑GTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTgg                                                                         <  2:1324989/139‑1 (MQ=255)                                            ccGCCCTCTCTCAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCG‑‑GTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTg                                                                       <  2:468782/139‑1 (MQ=255)                                            ccGCCCTCTCTCAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCG‑‑GTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTg                                                                       <  1:253101/139‑1 (MQ=255)                                            ccGCCCTCTCTCAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCG‑‑GTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTg                                                                       >  2:253101/1‑139 (MQ=255)                                               cccTCTCTCAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGc                                                                      >  1:16004/1‑139 (MQ=255)                                               cccTCTCTCAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCG‑‑GTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCgg                                                                    <  1:921184/139‑1 (MQ=255)                                                  tctctcAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCG‑‑GTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTac                                                                 <  1:1063991/139‑1 (MQ=255)                                                             cagcGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCG‑‑GTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACGGa                                                      >  1:487202/1‑139 (MQ=255)                                                              agcGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCG‑‑GTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTg                                                                          >  1:463328/1‑118 (MQ=255)                                                              agcGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCG‑‑GTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTg                                                                          <  2:463328/118‑1 (MQ=255)                                                              agcGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCG‑‑GTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCgg                                                                    <  1:1231149/124‑1 (MQ=255)                                                              agcGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCG‑‑GTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCgg                                                                    >  2:1231149/1‑124 (MQ=255)                                                              agcGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCG‑‑GTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACGGaa                                                     >  2:266824/1‑139 (MQ=255)                                                                          cacacTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCG‑‑GTGTGGTTGATGCa                                                                                                                  >  2:813759/1‑66 (MQ=255)                                                                          cacacTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCG‑‑GTGTGGTTGATGCa                                                                                                                  <  1:813759/66‑1 (MQ=255)                                                                          cacacTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCG‑‑GTGTGGTTGATGCACg                                                                                                                <  2:213840/68‑1 (MQ=255)                                                                          cacacTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCG‑‑GTGTGGTTGATGCACg                                                                                                                >  1:213840/1‑68 (MQ=255)                                                                             acTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCG‑‑GTGTGGTTGATGCACGAc                                                                                                              <  2:179127/67‑1 (MQ=255)                                                                             acTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCG‑‑GTGTGGTTGATGCACGAc                                                                                                              >  1:179127/1‑67 (MQ=255)                                                                             acTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCG‑‑GTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACGGAAGCCGGTGAActgctg                                      >  2:773322/1‑139 (MQ=255)                                                                               tGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCG‑‑GTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACGGAAGCCGGTGAACTGCTGGc                                    >  2:916203/1‑139 (MQ=255)                                                                                   cGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCG‑‑GGGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACGGAAGCCGGTGAACTGCTGGCGGta                                >  2:20250/1‑139 (MQ=255)                                                                                    ggTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCG‑‑GTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGc                                                                      >  1:269490/1‑100 (MQ=255)                                                                                    ggTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCG‑‑GTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGc                                                                      <  2:269490/100‑1 (MQ=255)                                                                                    ggTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCG‑‑GTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACGGAAGCCGGTGAACTGCTGGCGGtat                               <  2:487202/139‑1 (MQ=255)                                                                                     gTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCG‑‑GTGTGGTTGATGCACGAc                                                                                                              >  2:712228/1‑59 (MQ=255)                                                                                     gTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCG‑‑GTGTGGTTGATGCACGAc                                                                                                              <  1:712228/59‑1 (MQ=255)                                                                                      tACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCG‑‑GTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACGGAAGCCGGTGAACTGCTGGCGGTatat                             <  2:1316950/139‑1 (MQ=255)                                                                                           aaTACGGCCCCGACAGGGGTATCCGCCTTTCGGCG‑‑GTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTg                                                                                  >  2:1322780/1‑81 (MQ=255)                                                                                           aaTACGGCCCCGACAGGGGTATCCGCCTTTCGGCG‑‑GTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTg                                                                                  <  1:1322780/81‑1 (MQ=255)                                                                                           aaTACGGCCCCGACAGGGGTATCCGCCTTTCGGCG‑‑GTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTg                                                                       <  1:90713/92‑1 (MQ=255)                                                                                           aaTACGGCCCCGACAGGGGTATCCGCCTTTCGGCG‑‑GTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTg                                                                       >  2:90713/1‑92 (MQ=255)                                                                                           aaTACGGCCCCGACAGGGGTATCCGCCTTTCGGCG‑‑GTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACGGAAGCCGGTGAActgct                                       <  1:10237/124‑1 (MQ=255)                                                                                           aaTACGGCCCCGACAGGGGTATCCGCCTTTCGGCG‑‑GTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACGGAAGCCGGTGAActgct                                       >  2:10237/1‑124 (MQ=255)                                                                                           aaTACGGCCCCGACAGGGGTATCCGCCTTTCGGCG‑‑GTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACGGAAGCCGGTGAACTGCTGGCGGTATATGACCg                        <  2:346057/139‑1 (MQ=255)                                                                                           aaTACGGCCCCGACAGGGGTATCCGCCTTTCGGCG‑‑GTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACGGAAGCCGGTGAACTGCTGGCGGTATATGACCg                        >  1:346057/1‑139 (MQ=255)                                                                                                ggCCCCGACAGGGGTATCCGCCTTTCGGCG‑‑GTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGt                                                                        <  1:886959/86‑1 (MQ=255)                                                                                                ggCCCCGACAGGGGTATCCGCCTTTCGGCG‑‑GTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGt                                                                        >  2:886959/1‑86 (MQ=255)                                                                                                  ccccGACAGGGGTATCCGCCTTTCGGCG‑‑GTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGcc                                                                                       >  1:95545/1‑69 (MQ=255)                                                                                                  ccccGACAGGGGTATCCGCCTTTCGGCG‑‑GTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGcc                                                                                       <  2:95545/69‑1 (MQ=255)                                                                                                  ccccGACAGGGGTATCCGCCTTTCGGCG‑‑GTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCAc                                                                            >  2:479399/1‑80 (MQ=255)                                                                                                  ccccGACAGGGGTATCCGCCTTTCGGCG‑‑GTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCAc                                                                            <  1:479399/80‑1 (MQ=255)                                                                                                               aTCCGCCTTTCGGCG‑‑GTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACGGAAGCCGGTGAACTGCTGGCGGTATATGACCGCAGCAATACGCAGGTgcgcg    <  1:383250/139‑1 (MQ=255)                                                                                                                tCCGCCTTTCGGCG‑‑GTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACGGAAGCCGGTGAACTGCTGGCGGTATATGACCGCAGCAATACGCAGGTgcgcgc   >  2:714574/1‑139 (MQ=255)                                                                                                                 ccGCCTTTCGGCG‑‑GTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACGGAAGCCGGTGAACTGCTGGCGGTATATGACCGCAGCAATACGCAGGTGCGCGCt  >  2:1304382/1‑139 (MQ=255)                                                                                                                                      |                                                                                                                        CGGAAGAGGCCCGCACCTCTTCGCTATCTTCTTCTGACAGTTCCCGCCCTCTCTCAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCG‑‑GTGTGGCTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACGGAAGCCGGTGAACTGCTGGCGGTATATGACCGCAGCAATACGCAGGTGCGCGCT  >  NC_000913/524027‑524277

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑