Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 562,187 C→T Q831* (CAG→TAG)  sfmD → putative outer membrane export usher protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913562,1870CT100.0% 252.5 / NA 77Q831* (CAG→TAG) sfmDputative outer membrane export usher protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (43/34);  total (43/34)

TACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATGACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGAT  >  NC_000913/562057‑562319
                                                                                                                                  |                                                                                                                                    
tACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATGACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTTAGTGGGga                                                                                                                              >  2:4069/1‑139 (MQ=255)
       ggCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATGACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTTAGTGGGGAGACGgcg                                                                                                                       >  1:592603/1‑139 (MQ=255)
            aaaaCCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATGACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTTAGTGGGGAGACGGCGCGAAc                                                                                                                  <  2:1082283/139‑1 (MQ=255)
            aaaaCCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATGACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTTAGTGGGGAGACGGCGCGAAc                                                                                                                  <  2:146548/139‑1 (MQ=255)
                 cGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATGACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTTAGTGGGGAGACGGCGCGAACTCACg                                                                                                             >  1:939937/1‑139 (MQ=255)
                        gTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATGACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTTAGTGGGGAGACGGCGCGAACTCACGCTGCAtt                                                                                                      >  2:355420/1‑139 (MQ=255)
                         tttGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATGACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTTAGTGGGGAGACGGCGCGAACTCACGCTGCATTg                                                                                                     >  2:1378211/1‑139 (MQ=255)
                          ttGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATGACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTTAGTGGGGAGACGGCGCGAACTCACGCTGCATTgc                                                                                                    <  1:355420/139‑1 (MQ=255)
                             gATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATGACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTTAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCa                                                                                                 <  2:939937/139‑1 (MQ=255)
                             gATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATGACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTTAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCa                                                                                                 <  2:592603/139‑1 (MQ=255)
                             gATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATGACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTTAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCa                                                                                                 <  2:636581/139‑1 (MQ=255)
                               tCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATGACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTTAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACt                                                                                               <  1:511562/139‑1 (MQ=255)
                                       aCGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATGACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTTAGTGGGGAGACGGCGCGAACTCACGCTGCAt                                                                                                       <  2:627707/123‑1 (MQ=255)
                                       aCGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATGACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTTAGTGGGGAGACGGCGCGAACTCACGCTGCAt                                                                                                       >  1:627707/1‑123 (MQ=255)
                                          ggAAAACAGTACCGGAATAACCAGTATGGTGGGTGATGACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTTAGTGGGGAGACGGCGCGAACTCACGCTGCATTgc                                                                                                    <  2:851405/123‑1 (MQ=255)
                                          ggAAAACAGTACCGGAATAACCAGTATGGTGGGTGATGACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTTAGTGGGGAGACGGCGCGAACTCACGCTGCATTgc                                                                                                    >  1:851405/1‑123 (MQ=255)
                                                cAGTACCGGAATAACCAGTATGGTGGGTGATGACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTTAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCaa                                                                              >  1:549816/1‑139 (MQ=255)
                                                                 gTATGGTGGGTGATGACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTTAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGt                                                             <  2:549816/139‑1 (MQ=255)
                                                                 gTATGGTGGGTGATGACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTTAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGt                                                             <  2:1454283/139‑1 (MQ=255)
                                                                  tATGGTGGGTGATGACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTTAGTGGGGAGACGGCGCGa                                                                                                                    >  2:824418/1‑83 (MQ=255)
                                                                  tATGGTGGGTGATGACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTTAGTGGGGAGACGGCGCGa                                                                                                                    <  1:824418/83‑1 (MQ=255)
                                                                  tATGGTGGGTGATGACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTTAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTc                                                            >  2:418858/1‑139 (MQ=255)
                                                                  tATGGTGGGTGATGACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTTAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTc                                                            >  1:694314/1‑139 (MQ=255)
                                                                     ggtgggtgATGACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTTAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACt                                                         >  2:526061/1‑139 (MQ=255)
                                                                       tgggtgATGACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTTAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGt                                                       >  1:923945/1‑139 (MQ=255)
                                                                       agggtgATGACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTTAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGt                                                       >  1:918404/2‑139 (MQ=255)
                                                                         ggtgATGACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTTAGTGGGGAGACGGCGCGAACTCACGCTGCATTgc                                                                                                    >  1:1172082/1‑92 (MQ=255)
                                                                         ggtgATGACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTTAGTGGGGAGACGGCGCGAACTCACGCTGCATTgc                                                                                                    <  2:1172082/92‑1 (MQ=255)
                                                                          gtgATGACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTTAGTGGGGAGACGGCGCGAAc                                                                                                                  <  2:315459/77‑1 (MQ=255)
                                                                          gtgATGACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTTAGTGGGGAGACGGCGCGAAc                                                                                                                  >  1:315459/1‑77 (MQ=255)
                                                                            gatgaCGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTTAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCa                                                                                                 <  2:376386/92‑1 (MQ=255)
                                                                            gatgaCGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTTAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCa                                                                                                 >  1:376386/1‑92 (MQ=255)
                                                                            gatgaCGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTTAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTAttt                                                  >  2:149440/1‑139 (MQ=255)
                                                                            gatgaCGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTTAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTAttt                                                  >  2:936261/1‑139 (MQ=255)
                                                                            gatgaCGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTTAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTAttt                                                  >  2:1186897/1‑139 (MQ=255)
                                                                              tgaCGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTTAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCg                                                >  2:542915/1‑139 (MQ=255)
                                                                                aCGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTTAGTGGGGAGACGGCGCGAACTCACGCTGCATTgc                                                                                                    >  1:709831/1‑85 (MQ=255)
                                                                                aCGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTTAGTGGGGAGACGGCGCGAACTCACGCTGCATTgc                                                                                                    <  2:709831/85‑1 (MQ=255)
                                                                                aCGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTTAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGc                                              >  2:1059275/1‑139 (MQ=255)
                                                                                aCGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTTAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGc                                              >  1:84930/1‑139 (MQ=255)
                                                                                   ggCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTTAGTGGGGAGACGGCGCGAACTCACGCt                                                                                                           >  1:434413/1‑75 (MQ=255)
                                                                                   ggCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTTAGTGGGGAGACGGCGCGAACTCACGCt                                                                                                           <  2:434413/75‑1 (MQ=255)
                                                                                   ggCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTTAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCa                                                                                                 <  1:380471/85‑1 (MQ=255)
                                                                                   ggCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTTAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCa                                                                                                 >  2:380471/1‑85 (MQ=255)
                                                                                    gCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTTAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGc                                              <  2:675587/135‑1 (MQ=255)
                                                                                    gCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTTAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGc                                              >  1:675587/1‑135 (MQ=255)
                                                                                        gTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTTAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGca                                      <  1:149440/139‑1 (MQ=255)
                                                                                        gTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTAATGGTTTAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGca                                      <  2:923945/139‑1 (MQ=255)
                                                                                         tttatttaAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTTAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGcac                                     >  1:180355/1‑139 (MQ=255)
                                                                                              ttaAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTTAGTGGGGAGACGGCGCGAACTCACGCTGCATTgc                                                                                                    >  1:332260/1‑71 (MQ=255)
                                                                                              ttaAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTTAGTGGGGAGACGGCGCGAACTCACGCTGCATTgc                                                                                                    <  2:332260/71‑1 (MQ=255)
                                                                                                  gtggtgCGCCATTGTCTGGTGAATTACTGGTTTAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCa                                                                   >  1:620995/1‑100 (MQ=255)
                                                                                                  gtggtgCGCCATTGTCTGGTGAATTACTGGTTTAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCa                                                                   <  2:620995/100‑1 (MQ=255)
                                                                                                   tggtgCGCCATTGTCTGGTGAATTACTGGTTTAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCa                                                                               <  2:1014153/87‑1 (MQ=255)
                                                                                                   tggtgCGCCATTGTCTGGTGAATTACTGGTTTAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCa                                                                               >  1:1014153/1‑87 (MQ=255)
                                                                                                     gtgCGCCATTGTCTGGTGAATTACTGGTTTAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCg                                                >  1:593345/1‑116 (MQ=255)
                                                                                                     gtgCGCCATTGTCTGGTGAATTACTGGTTTAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCg                                                <  2:593345/116‑1 (MQ=255)
                                                                                                     gtgCGCCATTGTCTGGTGAATTACTGGTTTAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATc                                >  1:877248/1‑132 (MQ=255)
                                                                                                     gtgCGCCATTGTCTGGTGAATTACTGGTTTAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATc                                <  2:877248/132‑1 (MQ=255)
                                                                                                      tgCGCCATTGTCTGGTGAATTACTGGTTTAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCa                                                                                                 >  1:524271/1‑66 (MQ=255)
                                                                                                      tgCGCCATTGTCTGGTGAATTACTGGTTTAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCa                                                                                                 <  2:524271/66‑1 (MQ=255)
                                                                                                      tgCGCCATTGTCTGGTGAATTACTGGTTTAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCa                        >  1:152251/1‑139 (MQ=255)
                                                                                                       gcgcCATTGTCTGGTGAATTACTGGTTTAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTACTTCGGCAGTTTGca                                      <  1:680546/124‑1 (MQ=255)
                                                                                                       gcgcCATTGTCTGGTGAATTACTGGTTTAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTACTTCGGCAGTTTGca                                      >  2:680546/1‑124 (MQ=255)
                                                                                                        cgcCATTGTCTGGTGAATTACTGGTTTAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATa                      >  2:1084845/1‑139 (MQ=255)
                                                                                                               gTCTGGTGAATTACTGGTTTAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGaaa               >  1:742173/1‑139 (MQ=255)
                                                                                                                     tGAATTACTGGTTTAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCa         <  1:526061/139‑1 (MQ=255)
                                                                                                                       aaTTACTGGTTTAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCa         <  1:1325480/137‑1 (MQ=255)
                                                                                                                       aaTTACTGGTTTAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCa         >  2:1325480/1‑137 (MQ=255)
                                                                                                                       aaTTACTGGTTTAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAAt       >  2:1305616/1‑139 (MQ=255)
                                                                                                                        aTTACTGGTTTAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATa      <  1:1378211/139‑1 (MQ=255)
                                                                                                                        aTTACTGGTTTAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATa      <  1:1059275/139‑1 (MQ=255)
                                                                                                                         ttACTGGTTTAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGc                                                                           >  1:519936/1‑69 (MQ=255)
                                                                                                                         ttACTGGTTTAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGc                                                                           <  2:519936/69‑1 (MQ=255)
                                                                                                                          tACTGGTTTAGTGGGGAGACGGCGCGAACTCACGCTGCAtt                                                                                                      >  1:309824/1‑41 (MQ=255)
                                                                                                                          tACTGGTTTAGTGGGGAGACGGCGCGAACTCACGCTGCAtt                                                                                                      <  2:309824/41‑1 (MQ=255)
                                                                                                                            cTGGTTTAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGAt  >  2:1026686/1‑139 (MQ=255)
                                                                                                                                  |                                                                                                                                    
TACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATGACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGAT  >  NC_000913/562057‑562319

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑