Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	75,447	A→G	C12R (TGC→CGC)	thiB ←	thiamine/thiamine pyrophosphate/thiamine monophosphate ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	75,447	0	A	G	100.0%	149.7 / NA	42	C12R (TGC→CGC)	thiB	thiamine/thiamine pyrophosphate/thiamine monophosphate ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/21);  total (21/21)

CCACCAGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCG  >  NC_000913/75315‑75578                                                                                                                                     |                                                                                                                                    ccaccaGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCgcagcaa                                                                                                                               >  2:939630/1‑139 (MQ=255)               tCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGGCGCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGAc                                                                                                                 >  1:1476597/1‑139 (MQ=255)                 gCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAATCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACAt                                                                                                               <  2:945962/139‑1 (MQ=255)                  cAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACAtt                                                                                                              >  1:439481/1‑139 (MQ=255)                        cAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTa                                                                                                        <  1:703302/139‑1 (MQ=255)                               cTTCAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCgcagc                                                                                                                                 <  2:1096190/107‑1 (MQ=255)                               cTTCAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCgcagc                                                                                                                                 >  1:1096190/1‑107 (MQ=255)                                     aGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCt                                                                                           >  1:450467/1‑139 (MQ=255)                                                 ccaccGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACATTGCACCTCAAAAAAGAGTg                                                                               >  1:1237103/1‑139 (MQ=255)                                                    ccGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCa                                                                            <  1:939630/139‑1 (MQ=255)                                                       gACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAg                                                                         <  2:450467/139‑1 (MQ=255)                                                       gACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAAGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGGGGCAAAg                                                                         <  2:439481/139‑1 (MQ=255)                                                                  cAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCaa                                                                           <  2:549265/126‑1 (MQ=255)                                                                  cAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCaa                                                                           >  1:549265/1‑126 (MQ=255)                                                                  cAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAg                                                              >  2:1232990/1‑139 (MQ=255)                                                                       ggcggcGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCgcag                                                                                                                                  <  1:159227/66‑1 (MQ=255)                                                                       ggcggcGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCgcag                                                                                                                                  >  2:159227/1‑66 (MQ=255)                                                                       ggcggcGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGcc                                                         >  2:572626/1‑139 (MQ=255)                                                                               aGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTg                                                                                                <  1:49277/92‑1 (MQ=255)                                                                               aGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTg                                                                                                >  2:49277/1‑92 (MQ=255)                                                                                     cGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGc                                           <  1:556896/139‑1 (MQ=255)                                                                                          gTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCg                                      <  1:1467467/139‑1 (MQ=255)                                                                                            ataAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCgcag                                                                                                                                  <  1:772803/45‑1 (MQ=255)                                                                                            ataAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCgcag                                                                                                                                  >  2:772803/1‑45 (MQ=255)                                                                                              aaaCAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAACAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTAt                                  <  2:153929/139‑1 (MQ=255)                                                                                                  agccAGAACGGGCTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTCGAGAAGGAGCCTCAAATTCCTACGCCGGCGTTATCCgg                              >  1:1211480/4‑139 (MQ=255)                                                                                                    tCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCaa                                                                           <  1:860885/92‑1 (MQ=255)                                                                                                    tCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCaa                                                                           >  2:860885/1‑92 (MQ=255)                                                                                                    tCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGAt                            >  2:9276/1‑139 (MQ=255)                                                                                                    tCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGAt                            >  1:1259649/1‑139 (MQ=255)                                                                                                    tCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGAt                            >  1:1465154/1‑139 (MQ=255)                                                                                                    tCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTT‑ACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCg                                            >  2:1358782/1‑122 (MQ=255)                                                                                                    tCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTT‑ACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCg                                            <  1:1358782/122‑1 (MQ=255)                                                                                                     cAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGc                                       >  1:69577/1‑127 (MQ=255)                                                                                                     cAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGc                                       <  2:69577/127‑1 (MQ=255)                                                                                                     cAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATc                           >  2:813181/1‑139 (MQ=255)                                                                                                            ggTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAgg                                                                        <  2:1299698/87‑1 (MQ=255)                                                                                                            ggTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAgg                                                                        >  1:1299698/1‑87 (MQ=255)                                                                                                             gTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCa                                                                            <  2:613623/82‑1 (MQ=255)                                                                                                             gTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCa                                                                            >  1:613623/1‑82 (MQ=255)                                                                                                             gTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGa                   <  1:1232990/139‑1 (MQ=255)                                                                                                              tttAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGAc                  >  1:1424014/1‑139 (MQ=255)                                                                                                               ttAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACg                 <  2:1465154/139‑1 (MQ=255)                                                                                                                  gCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGa                                                                       >  2:1157829/1‑82 (MQ=255)                                                                                                                  gCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGa                                                                       <  1:1157829/82‑1 (MQ=255)                                                                                                                       aaCGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTg                                                                   <  1:232724/81‑1 (MQ=255)                                                                                                                       aaCGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTg                                                                   >  2:232724/1‑81 (MQ=255)                                                                                                                           ggCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCa     <  2:1424014/139‑1 (MQ=255)                                                                                                                              gcTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAgcg  <  1:572626/139‑1 (MQ=255)                                                                                                                                     |                                                                                                                                    CCACCAGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCG  >  NC_000913/75315‑75578

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑