| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 828,477 | C→T | V169I (GTT→ATT) | ybhF ← | putative ABC transporter ATPase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 828,477 | 0 | C | T | 97.9% | 152.7 / NA | 48 | V169I (GTT→ATT) | ybhF | putative ABC transporter ATPase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (24/23); minor base G (0/1); total (24/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTTCGTTCATCAGTAACACGTCACGGCACTGCTCGGCTTCGTCGAGATACGAGGTACTCCAGAGGATTAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAACGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTAAACGGCCCAAGAGACGTAAACT > NC_000913/828345‑828611 | cTTCGTTCATCAGTAACACGTCACGGCACTGCTCGGCTTCGTCGAGATACGAGGTACTCCAGAGGATTAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAatgccgac > 2:1438635/1‑139 (MQ=255)cTTCGTTCATCAGTAACACGTCACGGCACTGCTCGGCTTCGTCGAGATACGAGGTACTCCAGAGGATTAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAatgccgac < 2:360665/139‑1 (MQ=255) atcaGTAACACGTCACGGCACTGCTCGGCTTCGTCGAGATACGAGGTACTCCAGAGGATTAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGtt > 2:1408685/1‑139 (MQ=255) cacGTCACGGCACTGCTCGGCTTCGTCGAGATACGAGGTACTCCAGAGGATTAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGagc > 2:686361/1‑139 (MQ=255) cGTCACGGCACTGCTCGGCTTCGTCGAGATACGAGGTACTCCAGAGGATTAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGagcag > 1:777790/1‑139 (MQ=255) tGCTCGGCTTCGACGAGATACGAGGTCCTCCATAGGATTAACATCCCTTCGCCCGCCAGCACATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAAGGCCGACGCCGGCTTCATCGAGCAGCAACACTTTCg < 1:287940/139‑1 (MQ=255) cTCGGCTTCGTCGAGATACGAGGTACTCCAGAGGATTAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGt > 2:1441422/1‑139 (MQ=255) cGGCTTCGTCGAGATACGAGGTACTCCAGAGGATTAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTc < 1:1408685/139‑1 (MQ=255) cTTCGTCGAGATACGAGGTACTCCAGAGGATTAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGcc < 1:668499/139‑1 (MQ=255) cTTCGTCGAGATACGAGGTACTCCAGAGGATTAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGcc > 2:668499/1‑139 (MQ=255) cTTCGTCGAGATACGAGGTACTCCAGAGGATTAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGcc < 1:1438635/139‑1 (MQ=255) aTACGAGGTACTCCAGAGGATTAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTa < 1:137529/139‑1 (MQ=255) aTACGAGGTACTCCAGAGGATTAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTa < 1:1441422/139‑1 (MQ=255) cTCCAGAGGATTAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGAc < 2:777790/139‑1 (MQ=255) tCCAGAGGATTAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGAcc > 1:687759/1‑139 (MQ=255) cAGAGGATTAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTa < 1:1350920/126‑1 (MQ=255) cAGAGGATTAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTa > 2:1350920/1‑126 (MQ=255) agGATTAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGtt > 2:443662/1‑139 (MQ=255) gATTAACATCCCTTCGCCGGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGtttt > 1:816316/1‑139 (MQ=255) gATTAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCg < 1:1345279/109‑1 (MQ=255) gATTAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCg > 2:1345279/1‑109 (MQ=255) gATTAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGtttt > 1:570351/1‑139 (MQ=255) ttAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCcca < 1:1277509/111‑1 (MQ=255) ttAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCcca > 2:1277509/1‑111 (MQ=255) ttAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGTTTTTg > 1:1247158/1‑139 (MQ=255) tAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCccacc < 2:302291/112‑1 (MQ=255) tAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCccacc > 1:302291/1‑112 (MQ=255) tAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGTTTTTGt > 2:1134624/1‑139 (MQ=255) ccTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGTaa > 2:780444/1‑86 (MQ=255) ccTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCaa < 1:780444/86‑1 (MQ=255) ccTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGTTTTTGTTTCATcc < 1:1134624/139‑1 (MQ=255) cTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCg < 1:1269884/98‑1 (MQ=255) cTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCg > 2:1269884/1‑98 (MQ=255) cgcccgccAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGTTTTTGTTTCATCCCAcc > 2:759722/1‑139 (MQ=255) cgcccgccAGCTCATGCACCATCTGCCACAG‑TCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGTTTTTGTTTCATCCCACCg < 1:677064/139‑1 (MQ=255) gccAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGTTTTTGTTTCATCCCACCGgaga < 1:759722/139‑1 (MQ=255) cTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTg < 2:204180/139‑1 (MQ=255) gCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGTTTTTGTTTCAt > 2:207243/1‑118 (MQ=255) gCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGTTTTTGTTTCAt < 1:207243/118‑1 (MQ=255) ccACAGTTCGCGCCATGAGATAGGGTCAATGCCGACGCCGGGTTCATCg > 1:1335040/1‑49 (MQ=255) ccACAGTTCGCGCCATGAGATAGGGTCAATGCCGACGCCGGGTTCATCg < 2:1335040/49‑1 (MQ=255) gTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTaaa < 1:443662/139‑1 (MQ=255) gTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTaaa < 2:687759/139‑1 (MQ=255) ttCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGcc > 2:1477598/1‑66 (MQ=255) ttCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGcc < 1:1477598/66‑1 (MQ=255) cgcCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCaa > 1:1315743/1‑47 (MQ=255) cgcCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCaa < 2:1315743/47‑1 (MQ=255) cgcCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCccac < 2:445737/65‑1 (MQ=255) cgcCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCccac > 1:445737/1‑65 (MQ=255) tCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGGGTCCGGTAAACGGCCCAAGAGACGTAAACt > 2:1302128/1‑139 (MQ=255) | CTTCGTTCATCAGTAACACGTCACGGCACTGCTCGGCTTCGTCGAGATACGAGGTACTCCAGAGGATTAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAACGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTAAACGGCCCAAGAGACGTAAACT > NC_000913/828345‑828611 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |