Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	860,638	A→G	Y657H (TAT→CAT)	ybiW ←	putative pyruvate formate lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	860,638	0	A	G	100.0%	190.5 / NA	53	Y657H (TAT→CAT)	ybiW	putative pyruvate formate lyase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/27);  total (26/27)

GGTCAGTACCGGAGGCCGGGCTTGCGCCTTCTGCCAGCGGGGTGTGGGCTTTACGCCCGTCCGGTGTTGCCATAGTCTGCGCGCCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCATCGTCGTTGCCGTACTTCGGCGC  >  NC_000913/860512‑860764                                                                                                                               |                                                                                                                               ggTCAGTACCGGAGGCCGGGCTTGCGCCTTCTGCCAGCGGGGTGTGGGCTTTACGCCCGTCCGGTGTTGCCATAGTCTGCGCGCCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCAAc                                                                                                                    <  2:414417/139‑1 (MQ=255)            gAGGCCGGGCTTGCGCCTTCTGCCAGCGGGGTGTGGGCTTTACGCCCGTCCGGTGTTGCCATAGTCTGCGCGCCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGAc                                                                                                         >  1:480835/1‑139 (MQ=255)                 cGGGCTTGCGCCTTCTGCCAGCGGGGTGTGGGCTTTACGCCCGTCCGGTGTTGCCATAGTCTGCGCGCCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAg                                                                                                    >  2:878912/1‑139 (MQ=255)                 cGGGCTTGCGCCTTCTGCCAGCGGGGTGTGGGCTTTACGCCCGTCCGGTGTTGCCATAGTCTGCGCGCCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAg                                                                                                    >  1:577971/1‑139 (MQ=255)                   ggCTTGCGCCTTCTGCCAGCGGGGTGTGGGCTTTACGCCCGTCCGGTGTTGCCATAGTCTGCGCGCCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAgcg                                                                                                  >  1:1385585/1‑139 (MQ=255)                      ttGCGCCTTCTGCCAGCGGGGTGTGGGCTTTACGCCCGTCCGGTGTTGCCATAGTCTGCGCGCCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCgg                                                                                               <  2:1216203/139‑1 (MQ=255)                           ccTTCTGCCAGCGGGGTGTGGGCTTTACGCCCGTCCGGTGTTGCCATAGTCTGCGCGCCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTAt                                                                                          <  1:878912/139‑1 (MQ=255)                             ttCTGCCAGCGGGGTGTGGGCTTTACGCCCGTCCGGTGTTGCCATAGTCTGCGCGCCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATgg                                                                                        <  1:1337634/139‑1 (MQ=255)                              tCTGCCAGCGGGGTGTGGGCTTTACGCCCGTCCGGTGTTGCCATAGTCTGCGCGCCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAgc                                                                                                   >  1:17178/1‑127 (MQ=255)                              tCTGCCAGCGGGGTGTGGGCTTTACGCCCGTCCGGTGTTGCCATAGTCTGCGCGCCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAgc                                                                                                   <  2:17178/127‑1 (MQ=255)                               cTGCCAGCGGGGTGTGGGCTTTACGCCCGTCCGGTGTTGCCATAGTCTGCGCGCCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTa                                                                                      <  2:1385585/139‑1 (MQ=255)                                           tgtgGGCTTTACGCCCGTCCGGTGTTGCCATAGTCTGCGCGCCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTtc                                                                          >  2:1042866/1‑139 (MQ=255)                                                    tACGCCCGTCCGGTGTTGCCATAGTCTGCGCGCCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTa                                                                                      <  1:216693/118‑1 (MQ=255)                                                    tACGCCCGTCCGGTGTTGCCATAGTCTGCGCGCCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTa                                                                                      >  2:216693/1‑118 (MQ=255)                                                     aCGCCCGTCCGGTGTTGCCATAGTCTGCGCGCCAAACGGTACGTTAGCGGAGATTGCTGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCCGa                                                                                              <  1:850367/109‑1 (MQ=255)                                                     aCGCCCGTCCGGTGTTGCCATAGTCTGCGCGCCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGa                                                                                              >  2:850367/1‑109 (MQ=255)                                                           gTCCGGTGTTGCCATAGTCTGCGCGCCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGa                                                          >  1:828524/1‑139 (MQ=255)                                                                 tgtTGCCATAGTCTGCGCGCCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAgcg                                                    <  2:480835/139‑1 (MQ=255)                                                                 tgtTGCCATAGTCTGCGCGCCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAgcg                                                    <  2:577971/139‑1 (MQ=255)                                                                          aGTCTGCGCGCCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCcagc                                           <  2:523728/139‑1 (MQ=255)                                                                           gTCTGCGCGCCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTtcgt                                                                        <  2:1364020/109‑1 (MQ=255)                                                                           gTCTGCGCGCCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTtcgt                                                                        >  1:1364020/1‑109 (MQ=255)                                                                             cTGCGCGCCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTgcc                                                                                                                          >  1:1178843/1‑57 (MQ=255)                                                                             cTGCGCGCCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTgcc                                                                                                                          <  2:1178843/57‑1 (MQ=255)                                                                             cTGCGCGCCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCagcagc                                        >  2:1192688/1‑139 (MQ=255)                                                                              tGCGCGCCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCg                                       >  2:1248666/1‑139 (MQ=255)                                                                              tGCGCGCCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCg                                       >  1:331812/1‑139 (MQ=255)                                                                              tGCGCGCCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCg                                       >  2:172199/1‑139 (MQ=255)                                                                               gcgcgcCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGt                                      <  1:16983/139‑1 (MQ=255)                                                                                cgcgcCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGata                                                                   <  1:129793/109‑1 (MQ=255)                                                                                cgcgcCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGata                                                                   >  2:129793/1‑109 (MQ=255)                                                                                cgcgcCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTa                                     >  2:736864/1‑139 (MQ=255)                                                                                cgcgcCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTa                                     >  2:715357/1‑139 (MQ=255)                                                                                cgcgcCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTa                                     <  1:620769/139‑1 (MQ=255)                                                                                cgcgcCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTa                                     >  2:1335613/1‑139 (MQ=255)                                                                                cgcgcCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTa                                     >  1:246551/1‑139 (MQ=255)                                                                                  cgcCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATc                                   <  1:715357/139‑1 (MQ=255)                                                                                  cgcCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATc                                   <  2:331812/139‑1 (MQ=255)                                                                                    ccAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGa                                 <  1:172199/139‑1 (MQ=255)                                                                                                      gagaTTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCATCGTCGTTGc               >  1:783424/1‑139 (MQ=255)                                                                                                         aTTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTtcgt                                                                        >  1:42489/1‑79 (MQ=255)                                                                                                         aTTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTtcgt                                                                        <  2:42489/79‑1 (MQ=255)                                                                                                                 cGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTtcgt                                                                        <  2:449291/71‑1 (MQ=255)                                                                                                                 cGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTtcgt                                                                        >  1:449291/1‑71 (MQ=255)                                                                                                                 cGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGTGTATCGACAGTATCATCGTCGTTGCCGTACTTCGgc    >  2:1469573/1‑139 (MQ=255)                                                                                                                  gTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGTCAGCAGCGTATCGACAGTATCATCGTCGTTGCCGTACTTCGgcg   <  1:1042866/139‑1 (MQ=255)                                                                                                                  gTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGTGTATCGACAGTATCATCGTCGTTGCCGTACTTCGgcg   <  1:1469573/139‑1 (MQ=255)                                                                                                                  gTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCATCGTCGTTGCCGTACTTCGgcg   <  1:1335613/139‑1 (MQ=255)                                                                                                                  gTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCATCGTCGTTGCCGTACTTCGgcg   <  1:860891/139‑1 (MQ=255)                                                                                                                  gTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCATCGTCGTTGCCGTACTTCGgca   <  1:1248666/139‑2 (MQ=255)                                                                                                                   tACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCATCGTCGTTGCCGTACTTCGgcgc  >  2:1389786/1‑139 (MQ=255)                                                                                                                     cccGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCATCGTCGTTGCCGTACTTCGgcg   <  2:882583/136‑1 (MQ=255)                                                                                                                     cccGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCATCGTCGTTGCCGTACTTCGgcg   >  1:882583/1‑136 (MQ=255)                                                                                                                               |                                                                                                                               GGTCAGTACCGGAGGCCGGGCTTGCGCCTTCTGCCAGCGGGGTGTGGGCTTTACGCCCGTCCGGTGTTGCCATAGTCTGCGCGCCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCATCGTCGTTGCCGTACTTCGGCGC  >  NC_000913/860512‑860764

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑