Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	922,366	C→T	*75* (TAG→TAA)	cspD ←	inhibitor of DNA replication, cold shock protein homolog

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	922,366	0	C	T	100.0%	205.6 / NA	64	*75* (TAG→TAA)	cspD	inhibitor of DNA replication, cold shock protein homolog
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (33/31);  total (33/31)

ACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTCCAGCTTTTAGCGTTCTGTAACCATCCATCTGAATGGT  >  NC_000913/922257‑922494                                                                                                              |                                                                                                                                 aCGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACggg                                                                                                     <  2:1227805/139‑1 (MQ=255) aCGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACggg                                                                                                     <  1:21150/139‑1 (MQ=255)              gTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTg                                                                                        >  1:907316/1‑139 (MQ=255)               tAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGTTATGCGa                                                                                                                           >  2:1324077/1‑103 (MQ=255)               tAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGTTATGCGa                                                                                                                           <  1:1324077/103‑1 (MQ=255)                         ggCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGTTa                                                                                                                                >  2:1124754/1‑88 (MQ=255)                         ggCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACg                                                                                                       >  1:1443236/1‑113 (MQ=255)                         ggCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACg                                                                                                       <  2:1443236/113‑1 (MQ=255)                         ggCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGcc                                                                             >  2:667015/1‑139 (MQ=255)                         ggCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGcc                                                                             >  2:275382/1‑139 (MQ=255)                           cACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCtt                                                                           <  1:667015/139‑1 (MQ=255)                               agagTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACg                                                                                                       <  2:278809/107‑1 (MQ=255)                               agagTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACg                                                                                                       >  1:278809/1‑107 (MQ=255)                                    aaTTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAac                                                                                            <  1:810588/113‑1 (MQ=255)                                    aaTTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAac                                                                                            >  2:810588/1‑113 (MQ=255)                                           gagaTAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACg                                                                                                       >  2:840023/1‑95 (MQ=255)                                           gagaTAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACg                                                                                                       <  1:840023/95‑1 (MQ=255)                                              aTAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATc                                                        >  2:1252507/1‑139 (MQ=255)                                                     tGCCAGCCGATCGGGGTGGCATTTGGCCTTTAGGATGTACACAATGAGACAGAAGGGTTATGCGACTGCCGCTTCTACTTGGACGGGCACAAGAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGa                                                 <  1:1446765/139‑1 (MQ=255)                                                      gCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGTTATGCGACTGc                                                                                                                       <  1:727962/68‑1 (MQ=255)                                                      gCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGTTATGCGACTGc                                                                                                                       >  2:727962/1‑68 (MQ=255)                                                                  ggCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGa                                                                                                         <  2:14140/70‑1 (MQ=255)                                                                  ggCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGa                                                                                                         >  1:14140/1‑70 (MQ=255)                                                                      ggCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTCCAGCttt                                >  1:705625/1‑139 (MQ=255)                                                                      ggCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTCCAGCttt                                >  1:1029271/1‑139 (MQ=255)                                                                        cATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGc                                                                      >  1:555770/1‑99 (MQ=255)                                                                        cATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGc                                                                      <  2:555770/99‑1 (MQ=255)                                                                         aTTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTCCAGCTTTTAg                             <  1:275382/139‑1 (MQ=255)                                                                           tttGCCTTTAGGATGTACACAATGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTCCAGCTTTTAGCg                           <  1:1252507/139‑1 (MQ=255)                                                                             tGCCTTTAGGATGTACACAATGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGAc                                                                                                        >  2:258528/1‑60 (MQ=255)                                                                             tGCCTTTAGGATGTACACAATGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGAc                                                                                                        <  1:258528/60‑1 (MQ=255)                                                                                    aGGATGTACACAATGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTCCAGCTTTTAGc                            >  2:1104652/1‑129 (MQ=255)                                                                                    aGGATGTACACAATGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTCCAGCTTTTAGc                            <  1:1104652/129‑1 (MQ=255)                                                                                          tACACAATGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACg                                                                                                       >  2:538232/1‑48 (MQ=255)                                                                                          tACACAATGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACg                                                                                                       <  1:538232/48‑1 (MQ=255)                                                                                          tACACAATGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTCCAGCTTTTAGCGTTCTGTAAccatcca            >  2:598183/1‑139 (MQ=255)                                                                                           acacAATGAGATAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACggg                                                                                                     <  1:178745/49‑1 (MQ=38)                                                                                           acacAATGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACggg                                                                                                     >  2:178745/1‑49 (MQ=255)                                                                                            cacaATGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTCCAGCTTTTAGCGTTCTGTAACcatccatc          >  1:1105124/1‑139 (MQ=255)                                                                                               aaTGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTCCAGCTTTTAGCGTTCTGTAACCATCCATCTGa       >  1:850049/1‑139 (MQ=255)                                                                                               aaTGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTCCAGCTTTTAGCGTTCTGTAACCATCCATCTGa       >  2:1303193/1‑139 (MQ=255)                                                                                                aTGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCa                                                       >  2:111254/1‑90 (MQ=255)                                                                                                aTGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCa                                                       <  1:111254/90‑1 (MQ=255)                                                                                                aTGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTCCAGCTTTTAGCGTTCTGTAACCATCCATCTGaa      <  2:1029271/139‑1 (MQ=255)                                                                                                aTGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTCCAGCTTTTAGCGTTCTGTAACCATCCATCTGaa      <  1:598183/139‑1 (MQ=255)                                                                                                 tGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTCCAGCTTTTAGCGTTCTGTAAccat               <  1:383791/129‑1 (MQ=255)                                                                                                 tGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTCCAGCTTTTAGCGTTCTGTAAccat               >  2:383791/1‑129 (MQ=255)                                                                                                 tGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTCCAGCTTTTAGCGTTCTGTAAccat               <  1:1411396/129‑1 (MQ=255)                                                                                                 tGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTCCAGCTTTTAGCGTTCTGTAAccat               >  2:1411396/1‑129 (MQ=255)                                                                                                  gagaCAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTCCAGCTTTTAGCGTTCTGTAACCATCCATCTGAAtg    <  1:1303193/139‑1 (MQ=255)                                                                                                    gaCAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCttt                                                                          <  1:1149532/67‑1 (MQ=255)                                                                                                    gaCAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCttt                                                                          >  2:1149532/1‑67 (MQ=255)                                                                                                    gaCAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATc                                                        >  1:408656/1‑85 (MQ=255)                                                                                                    gaCAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATc                                                        <  2:408656/85‑1 (MQ=255)                                                                                                    gaCAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTCCAGCTTTTAGCGTTCTGTAACCATCCATCTGAAtggt  >  1:409799/1‑139 (MQ=255)                                                                                                    gaCAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTCCAGCTTTTAGCGTTCTGTAACCATCCATCTGAAtggt  <  2:705625/139‑1 (MQ=255)                                                                                                       agaagaGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTCCAGCTTTTAGCGTTCTGTAACCATCCATCTGaa      <  2:1166328/132‑1 (MQ=255)                                                                                                       agaagaGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTCCAGCTTTTAGCGTTCTGTAACCATCCATCTGaa      >  1:1166328/1‑132 (MQ=255)                                                                                                         aagaGTTATGCGACTGCCGCTTCTACTTCGACggg                                                                                                     >  2:96234/1‑35 (MQ=38)                                                                                                         aagaGTTATGCGACTGCCGCTTCTACTTCGACggg                                                                                                     <  1:96234/35‑1 (MQ=38)                                                                                                         aagaGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCaa                                                      >  2:315128/1‑82 (MQ=255)                                                                                                         aagaGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCaa                                                      <  1:315128/82‑1 (MQ=255)                                                                                                         aagaGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTCCAGCTTTTa                              >  2:456772/1‑106 (MQ=255)                                                                                                         aagaGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTCCAGCTTTTa                              <  1:456772/106‑1 (MQ=255)                                                                                                              |                                                                                                                                 ACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTCCAGCTTTTAGCGTTCTGTAACCATCCATCTGAATGGT  >  NC_000913/922257‑922494

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑