Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 104,352 | A→G | E124G (GAG→GGG) | ftsA → | ATP‑binding cell division FtsK recruitment protein |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 104,352 | 0 | A | G | 97.3% | 250.5 / ‑3.0 | 73 | E124G (GAG→GGG) | ftsA | ATP‑binding cell division FtsK recruitment protein |
Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (33/38); total (34/39) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.21e-01 |
TATCTGGCGCTTTCTGGTAAGCACATCAGCTGCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGAGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGATATGGCG > NC_000913/104219‑104485 | tatCTGGCGCTTTCTGGTAAGCACATCAGCTGCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATc > 1:636969/1‑139 (MQ=255) ggCGCTTTCTGGTAAGCACATCAGCTGCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGtgtg < 2:213257/139‑1 (MQ=255) ggCGCTTTCTGGTAAGCACATCAGCTGCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGtgtg < 2:54596/139‑1 (MQ=255) tCTGGTAAGCACATCAGCTGCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCgtg < 1:1015074/130‑1 (MQ=255) tCTGGTAAGCACATCAGCTGCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCgtg > 2:1015074/1‑130 (MQ=255) tCTGGTAAGCACATCAGCTGCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCAtg > 1:685738/1‑139 (MQ=255) tCTGGTAAGCACATCAGCTGCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCAtg > 1:797786/1‑139 (MQ=255) ggTAAGCACATCAGCTGCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGa < 2:540349/139‑1 (MQ=255) caTCAGCTGCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCaa < 2:797648/139‑1 (MQ=255) aGCTGCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGt < 1:683799/139‑1 (MQ=255) gCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGtgtg > 2:1325423/1‑113 (MQ=255) gCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGtgtg < 1:1325423/113‑1 (MQ=255) gCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGc > 2:614967/1‑139 (MQ=255) cAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGGTGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGa < 1:1221947/139‑1 (MQ=255) gAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCgtg < 1:85034/107‑1 (MQ=255) gAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCgtg > 2:85034/1‑107 (MQ=255) gAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGAtt < 1:659865/139‑1 (MQ=255) gAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACt < 1:614967/139‑1 (MQ=255) aaTTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTAt < 2:901149/139‑1 (MQ=255) gTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGa > 1:3663/1‑139 (MQ=255) tGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAgg < 1:1341431/139‑1 (MQ=255) ggTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTAt > 2:688136/1‑130 (MQ=255) ggTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTAt < 1:688136/130‑1 (MQ=255) ccTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCa > 2:885713/1‑139 (MQ=255) tATTTCTGAAGAAGAAGTGACGCAAGAGGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCATGAAGGGATCAAg > 1:876444/1‑139 (MQ=255) tATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCgtg > 2:1078557/1‑86 (MQ=255) tATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCgtg < 1:1078557/86‑1 (MQ=255) aTTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGa > 2:488639/1‑139 (MQ=255) aTTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGa > 2:931386/1‑139 (MQ=255) tttCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGaa < 2:636969/139‑1 (MQ=255) tCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATc < 1:931386/139‑1 (MQ=255) gaagaagaaGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCgg > 1:1018336/1‑139 (MQ=255) gaagTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGa < 1:33284/85‑1 (MQ=255) gaagTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGa > 2:33284/1‑85 (MQ=255) cGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCggg < 1:339829/139‑1 (MQ=255) gCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGa > 1:502523/1‑77 (MQ=255) gCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGa < 2:502523/77‑1 (MQ=255) gCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAgg < 1:595525/129‑1 (MQ=255) gCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAgg > 2:595525/1‑129 (MQ=255) agaagaTGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTg > 1:1351756/1‑139 (MQ=255) gtgGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCgg > 1:517958/1‑115 (MQ=255) gtgGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCgg < 2:517958/115‑1 (MQ=255) tgGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCa < 2:1018336/139‑1 (MQ=255) gAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAgg < 2:685738/139‑1 (MQ=255) aaaaCGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGc < 1:885713/139‑1 (MQ=255) aaaCGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGtgct < 1:19440/54‑1 (MQ=255) aaaCGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGtgct > 2:19440/1‑54 (MQ=255) cgtcgtCCATACCACGAAATCGGTGCGTGTGCGCGATGAGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGc > 1:442221/1‑75 (MQ=255) cgtcgtCCATACCACGAAATCGGTGCGTGTGCGCGATGAGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGc < 2:442221/75‑1 (MQ=255) tcCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCg > 1:333331/1‑117 (MQ=255) tcCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCg < 2:333331/117‑1 (MQ=255) tcCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCa < 2:499129/127‑1 (MQ=255) tcCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCa > 1:499129/1‑127 (MQ=255) cATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCAcc < 1:488639/139‑1 (MQ=255) aCCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGtgtg < 2:835112/39‑1 (MQ=38) aCCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGtgtg > 1:835112/1‑39 (MQ=255) aCCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCacatgtgatcaggt < 2:1351756/139‑14 (MQ=255) ccGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGAt > 2:1421904/1‑139 (MQ=255) cgAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAg > 1:1234738/1‑78 (MQ=255) cgAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAg < 2:1234738/78‑1 (MQ=255) gAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAggg > 2:442655/1‑79 (MQ=255) gAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAggg < 1:442655/79‑1 (MQ=255) gAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATca < 1:333125/137‑1 (MQ=255) gAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATca > 2:333125/1‑137 (MQ=255) aaaTCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGa > 2:655383/1‑65 (MQ=255) aaaTCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGa < 1:655383/65‑1 (MQ=255) tgtgCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTa > 2:1441950/1‑82 (MQ=255) tgtgCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTa < 1:1441950/82‑1 (MQ=255) tgtgCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCa > 2:1217375/1‑117 (MQ=255) tgtgCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCa < 1:1217375/117‑1 (MQ=255) gcgcGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGATATg < 1:1421904/139‑1 (MQ=255) cgATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGATATGGCg > 1:305623/1‑139 (MQ=255) aTGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTg > 1:1348015/1‑107 (MQ=255) | TATCTGGCGCTTTCTGGTAAGCACATCAGCTGCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGAGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGATATGGCG > NC_000913/104219‑104485 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |