Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,271,455	G→A	T132I (ACC→ATC)	chaA ←	calcium/sodium:proton antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,271,455	0	G	A	100.0%	163.0 / NA	51	T132I (ACC→ATC)	chaA	calcium/sodium:proton antiporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/23);  total (28/23)

CTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGGTGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGTAACAATCATAATGATTGAATAGAGCGTATCACGCATTAGCGTTGGCGCGGCGTCGCCGGTTGCCATTAAAGCTGAAATCAAA  >  NC_000913/1271340‑1271589                                                                                                                    |                                                                                                                                       cTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCcaa                                                                                                                  <  2:1449036/138‑1 (MQ=255) cTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCcaa                                                                                                                  >  1:1449036/1‑138 (MQ=255)         ttGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGa                                                                                                         >  2:894891/1‑139 (MQ=255)                  tCGCCGCAGGCAGGGCCATTGGAAATACCAGTACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAAc                                                                                                <  1:552034/139‑1 (MQ=255)                              gagCCATTGGAAATACCAGTACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGt                                                                                    <  1:1214687/139‑1 (MQ=255)                              gagCCATTGGAAATACCAGTACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGt                                                                                    <  1:1436053/139‑1 (MQ=255)                                gCCATTGGAAATACCAGTACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGTaa                                                                                  >  2:280092/1‑139 (MQ=255)                                    ttGGAAATACCAGTACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGTAACAAt                                                                              <  2:257974/139‑1 (MQ=255)                                           tACCAGTACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCg                                                                                                                       <  2:5088/91‑1 (MQ=255)                                           tACCAGTACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCg                                                                                                                       >  1:5088/1‑91 (MQ=255)                                                gTACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGTAACAATCATAATGATTGa                                                                  <  1:894891/139‑1 (MQ=255)                                                  aCGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAAc                                                                                                <  1:228472/107‑1 (MQ=255)                                                  aCGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAAc                                                                                                >  2:228472/1‑107 (MQ=255)                                                      ttATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGa                                                                                                                          >  1:827890/1‑77 (MQ=255)                                                      ttATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGa                                                                                                                          <  2:827890/77‑1 (MQ=255)                                                      ttATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGTAACa                                                                                >  2:330986/1‑119 (MQ=255)                                                      ttATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGTAACa                                                                                <  1:330986/119‑1 (MQ=255)                                                        aTCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGTAACAATCATAATGATTGAATAGAGCg                                                          >  2:739071/1‑139 (MQ=255)                                                              aGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCcaaca                                                                                                                >  2:267159/1‑79 (MQ=255)                                                              aGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCcaaca                                                                                                                <  1:267159/79‑1 (MQ=255)                                                                ggggAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGt                                                                                    >  1:1296239/1‑105 (MQ=255)                                                                ggggAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGt                                                                                    <  2:1296239/105‑1 (MQ=255)                                                                                   tACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGTAACAATCATAATGATTGAATAGAGCGTATCACGCATTAGCGTTGGCGCGGCGt                               >  2:766606/1‑139 (MQ=255)                                                                                   tACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGTAACAATCATAATGATTGAATAGAGCGTATCACGCATTAGCGTTGGCGCGGCGt                               <  1:766606/139‑1 (MQ=255)                                                                                      tGCTTGATATCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAAc                                                                                                <  1:949258/71‑1 (MQ=255)                                                                                      tGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAAc                                                                                                >  2:949258/1‑71 (MQ=255)                                                                                        cTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGTAACAATCATAATGATTGAATAGAGCGTATCACGCATTAGCGTTGGCGCGGCGTCGCCg                          >  1:1385493/1‑139 (MQ=255)                                                                                          tGATACCAAACAGATTTATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAAcc                                                                                               <  2:901006/68‑1 (MQ=255)                                                                                          tGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAAcc                                                                                               >  1:901006/1‑68 (MQ=255)                                                                                                cAAACAGATTCATATATTGGATGGCAAACTTACGACCGccc                                                                                                                    >  1:868803/1‑41 (MQ=255)                                                                                                cAAACAGATTCATATATTGGATGGCAAACTTACGACCGccc                                                                                                                    <  2:868803/41‑1 (MQ=255)                                                                                                cAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGTAACAATCATAATGATTGAATAGAGCGTATCACGCATTAGCGTTGGCGCGGCGTCGCCGGTTGCCAt                  >  2:339744/1‑139 (MQ=255)                                                                                                  aaCAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGt                                                                                    >  2:1093053/1‑71 (MQ=255)                                                                                                  aaCAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGt                                                                                    <  1:1093053/71‑1 (MQ=255)                                                                                                  aaCAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGTAACAATCATAATGATTGAATAGAGCGTATCACGCATTAGCGTTGGCGCGGCGTCGCCGGTTGCCATTa                >  1:177364/1‑139 (MQ=255)                                                                                                       aTTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGccc                                                                                          <  2:350225/60‑1 (MQ=255)                                                                                                       aTTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGccc                                                                                          >  1:350225/1‑60 (MQ=255)                                                                                                       aTTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGTaa                                                                                  <  2:478458/68‑1 (MQ=255)                                                                                                       aTTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGTaa                                                                                  >  1:478458/1‑68 (MQ=255)                                                                                                       aTTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGTAACAATCATAATGa                                                                      <  2:853012/80‑1 (MQ=255)                                                                                                       aTTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGTAACAATCATAATGATTGAATAGAGCGTATCACGCATTAGCGTTGGCGCGGCGTCGCCGGTTGCCATTAAAGCt           >  2:422609/1‑139 (MQ=255)                                                                                                       aTTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGTAACAATCATAATGATTGAATAGAGCGTATCACGCATTAGCGTTGGCGCGGCGTCGCCGGTTGCCATTAAAGCt           >  2:453648/1‑139 (MQ=255)                                                                                                       aTTCATATATTAGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGTAACAATCATAATGa                                                                      >  1:853012/1‑80 (MQ=255)                                                                                                        ttCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGTAACAATCATAATGATTGAATAGAGCGTATCACGCATTAGCGTTGGCGCGGCGTCGCCGGTTGCCATTAAAGCTg          <  1:739071/139‑1 (MQ=255)                                                                                                          cATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGTaa                                                                                  <  2:1324337/65‑1 (MQ=255)                                                                                                          cATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGTaa                                                                                  >  1:1324337/1‑65 (MQ=255)                                                                                                          cATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGTAACAATCATAATGATTGAATAGAGCGTATCACGCATTAGCGTTGGCGCGGCGTCGCCg                          >  1:956147/1‑121 (MQ=255)                                                                                                          cATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGTAACAATCATAATGATTGAATAGAGCGTATCACGCATTAGCGTTGGCGCGGCGTCGCCg                          <  2:956147/121‑1 (MQ=255)                                                                                                          cATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGTAACAATCATAATGATTGAATAGAGCGTATCACGCATTAGCGTTGGCGCGGCGTCGCCGGTTGCCATTAAAGCTGaa        >  2:980165/1‑139 (MQ=255)                                                                                                                 tGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCTGTAACAATCATaa                                                                         >  2:752277/1‑67 (MQ=255)                                                                                                                 tGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGTAACAATCATAATGATTGAATAGAGCGTATCACGCATTAGCGTTGGCGCGGCGTCGCCGGTTGCCATTAAAGCTGAAATCaaa  >  1:485149/1‑138 (MQ=255)                                                                                                                    |                                                                                                                                       CTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGGTGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGTAACAATCATAATGATTGAATAGAGCGTATCACGCATTAGCGTTGGCGCGGCGTCGCCGGTTGCCATTAAAGCTGAAATCAAA  >  NC_000913/1271340‑1271589

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑