Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	130,233	T→C	N343S (AAC→AGC)	yacH ←	DUF3300 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	130,233	0	T	C	100.0%	342.3 / NA	94	N343S (AAC→AGC)	yacH	DUF3300 family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (50/44);  total (50/44)

TTTGATGAAACCGCTTTGCCATATCCTGATCATGATAGGGCACACCATTACGGTAGTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATG  >  NC_000913/130111‑130361                                                                                                                           |                                                                                                                                 tttGATGAAACCGCTTTGCCATATCCTGATCATGATAGGGCACACCATTACGGTAGTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGAt                                                                                                                  >  2:787614/1‑139 (MQ=255)      tgaAACCGCTTTGCCATATCCTGATCATGATAGGGCACACCATTACGGTAGTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGAtgttgt                                                                                                             <  1:787614/139‑1 (MQ=255)         aaCCGCTTTGCCATATCCTGATCATGATAGGGCACACCATTACGGTAGTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGc                                                                                                          >  1:490543/1‑139 (MQ=255)                     atatCCTGATCATGATAGGGCACACCATTACGGTAGTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTgcc                                                                                              <  1:163666/139‑1 (MQ=255)                       atCCTGATCATGATAGGGCACACCATTACGGTAGTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTgccag                                                                                            >  2:478206/1‑139 (MQ=255)                        tCCTGATCATGATAGGGCACACCATTACGGTAGTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTg                                                                                                <  2:1392009/134‑1 (MQ=255)                        tCCTGATCATGATAGGGCACACCATTACGGTAGTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTg                                                                                                >  1:1392009/1‑134 (MQ=255)                             aTCATGATAGGGCACACCATTACGGTAGTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTa                                                                                      >  1:1285915/1‑139 (MQ=255)                                  gATAGGGCACACCATTACGGTAGTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTc                                                                                 >  2:925785/1‑139 (MQ=255)                                  gATAGGGCACACCATTACGGTAGTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTc                                                                                 >  2:1021725/1‑139 (MQ=255)                                       ggCACACCATTACGGTAGTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGAt                                                                            >  1:1332245/1‑139 (MQ=255)                                       ggCACACCATTACGGTAGTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGAt                                                                            >  1:729619/1‑139 (MQ=255)                                       ggCACACCATTACGGTAGTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGAt                                                                            >  1:645925/1‑139 (MQ=255)                                         cacaCCATTACGGTAGTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTtg                                                                                                     <  2:44794/112‑1 (MQ=255)                                         cacaCCATTACGGTAGTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTtg                                                                                                     >  1:44794/1‑112 (MQ=255)                                          acacCATTACGGTAGTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGAc                                                                         <  1:715195/139‑1 (MQ=255)                                            acCATTACGGTAGTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCg                                                                       >  1:1002781/1‑139 (MQ=255)                                            acCATTACGGTAGTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCg                                                                       >  1:41146/1‑139 (MQ=255)                                              cATTACGGTAGTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGcc                                                                     >  1:1323334/1‑139 (MQ=255)                                                ttACGGTAGTTTGGATTGTGTCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGt                                                                                                                            <  2:407544/82‑1 (MQ=255)                                                ttACGGTAGTTTGGATTGTGTCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGt                                                                                                                            >  1:407544/1‑82 (MQ=255)                                                   cGGTAGTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGGTGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCgt                                                                >  1:1039817/1‑139 (MQ=255)                                                      tAGTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCgtggt                                                             >  1:1408179/1‑139 (MQ=255)                                                      tAGTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCgtggt                                                             <  2:490543/139‑1 (MQ=255)                                                        gTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCa                                                                                >  1:386555/1‑118 (MQ=255)                                                        gTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCa                                                                                <  2:386555/118‑1 (MQ=255)                                                        gTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCgt                                                                >  2:321125/1‑134 (MQ=255)                                                        gTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCgt                                                                <  1:321125/134‑1 (MQ=255)                                                        gTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGtga                                                           >  1:268161/1‑139 (MQ=255)                                                        gTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGtga                                                           >  1:1259785/1‑139 (MQ=255)                                                        gTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGtga                                                           <  2:1310974/139‑1 (MQ=255)                                                        gTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGtga                                                           <  2:1002781/139‑1 (MQ=255)                                                        gTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGtga                                                           >  2:701174/1‑139 (MQ=255)                                                              aTTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATaa                                                     <  2:1332245/139‑1 (MQ=255)                                                               ttGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAat                                                    <  2:1285915/139‑1 (MQ=255)                                                                  tgCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCg                                                                                                           >  1:1155688/1‑81 (MQ=255)                                                                  tgCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCg                                                                                                           <  2:1155688/81‑1 (MQ=255)                                                                  tgCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAatcat                                                 >  1:670103/1‑139 (MQ=255)                                                                  tgCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAatcat                                                 >  1:358585/1‑139 (MQ=255)                                                                  ggccgccATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAatcat                                                 <  2:645705/138‑1 (MQ=255)                                                                     cgccATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATcatcat                                              <  2:268161/139‑1 (MQ=255)                                                                          tgccatATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGCGGTGATGATAATCATCATTGTCa                                         <  2:1323334/139‑1 (MQ=255)                                                                            ccatATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCAt                                                                                        >  2:15708/1‑90 (MQ=255)                                                                            ccatATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCAt                                                                                        <  1:15708/90‑1 (MQ=255)                                                                              atatTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCatgat                                     <  1:701174/139‑1 (MQ=255)                                                                                  tCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCatgatgatg                                 <  2:1408179/139‑1 (MQ=255)                                                                                  tCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCatgatgatg                                 <  2:645925/139‑1 (MQ=255)                                                                                      aTCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTc                                                                                                                           <  1:1303204/45‑1 (MQ=255)                                                                                      aTCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTc                                                                                                                           >  2:1303204/1‑45 (MQ=255)                                                                                      aTCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAat                                                    <  2:528933/116‑1 (MQ=255)                                                                                      aTCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAat                                                    >  1:528933/1‑116 (MQ=255)                                                                                      aTCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCatgatgatga                                <  2:1295154/136‑1 (MQ=255)                                                                                      aTCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCatgatgatga                                >  1:1295154/1‑136 (MQ=255)                                                                                      aTCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATgg                             >  2:675150/1‑139 (MQ=255)                                                                                       tCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGt                            <  1:478206/139‑1 (MQ=255)                                                                                          gTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCAt                         <  2:393279/139‑1 (MQ=255)                                                                                           tAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATg                        >  2:356419/1‑139 (MQ=255)                                                                                           tAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATg                        >  1:137579/1‑139 (MQ=255)                                                                                           tAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATg                        >  2:93922/1‑139 (MQ=255)                                                                                           tAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATg                        >  2:229089/1‑139 (MQ=255)                                                                                              gATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTa                                                                                      <  2:659919/74‑1 (MQ=255)                                                                                              gATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTa                                                                                      >  1:659919/1‑74 (MQ=255)                                                                                              gATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGAtc                     >  2:749369/1‑139 (MQ=255)                                                                                              gATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGAtc                     >  1:229899/1‑139 (MQ=255)                                                                                                  cTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCa                          <  2:197476/130‑1 (MQ=255)                                                                                                  cTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCa                          >  1:197476/1‑130 (MQ=255)                                                                                                   tCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGAtcgtcgt                <  2:729619/139‑1 (MQ=255)                                                                                                   tCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGAtcgtcgt                >  2:687323/1‑139 (MQ=255)                                                                                                   tCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGAt                      <  2:1091301/133‑1 (MQ=255)                                                                                                   tCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGAt                      >  1:1091301/1‑133 (MQ=255)                                                                                                     aCCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATc                                                                  >  1:441999/1‑87 (MQ=255)                                                                                                     aCCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATc                                                                  <  2:441999/87‑1 (MQ=255)                                                                                                     aCCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGAtcgtcgtcg              <  2:1259785/139‑1 (MQ=255)                                                                                                     aCCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGAtcgtcgtcg              >  2:57347/1‑139 (MQ=255)                                                                                                         gTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATcatcat                                              >  2:775453/1‑103 (MQ=255)                                                                                                         gTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATcatcat                                              <  1:775453/103‑1 (MQ=255)                                                                                                            aTACGGTTGAAATTGCTGACGTCGATATTTATGTTGTGGCCGTTGTGTAGACAGCCATTACCGTACCGATAACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGt       <  1:750810/139‑1 (MQ=255)                                                                                                            aTACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATc                                                                  <  1:576002/80‑1 (MQ=255)                                                                                                            aTACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATc                                                                  >  2:576002/1‑80 (MQ=255)                                                                                                            aTACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCgtg                                                               <  2:398779/83‑1 (MQ=255)                                                                                                            aTACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCgtg                                                               >  1:398779/1‑83 (MQ=255)                                                                                                            aTACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGtgat                                                          <  1:135154/88‑1 (MQ=255)                                                                                                            aTACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGtgat                                                          >  2:135154/1‑88 (MQ=255)                                                                                                            aTACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGt       >  1:146619/1‑139 (MQ=255)                                                                                                               cGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGa    <  2:1241368/139‑1 (MQ=255)                                                                                                               cGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGa    <  2:41146/139‑1 (MQ=255)                                                                                                               cGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGa    <  2:358585/139‑1 (MQ=255)                                                                                                               cGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGa    <  2:229899/139‑1 (MQ=255)                                                                                                               cGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGa    <  2:146619/139‑1 (MQ=255)                                                                                                               cGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGa    <  2:137579/139‑1 (MQ=255)                                                                                                                 gTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCa                                                                                <  2:541114/61‑1 (MQ=255)                                                                                                                 gTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCa                                                                                >  1:541114/1‑61 (MQ=255)                                                                                                                 gTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGAtg  >  2:440389/1‑139 (MQ=255)                                                                                                                    gAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCATCGCAGTCGAtg  <  2:987501/136‑1 (MQ=255)                                                                                                                    gAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCATCCCAGTCGAtg  >  1:987501/1‑136 (MQ=255)                                                                                                                           |                                                                                                                                 TTTGATGAAACCGCTTTGCCATATCCTGATCATGATAGGGCACACCATTACGGTAGTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATG  >  NC_000913/130111‑130361

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑