Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 155,935 T→C K89K (AAA→AAG)  yadV ← putative periplasmic pilin chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913155,9350TC100.0% 215.6 / NA 61K89K (AAA→AAG) yadVputative periplasmic pilin chaperone
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (32/29);  total (32/29)

CTTTGATTCGCGACCTTTTCTGCATCTGGTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGA  >  NC_000913/155807‑156064
                                                                                                                                |                                                                                                                                 
cTTTGATTCGCGACCTTTTCTGCATCTGGTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCaa                                                                                                                         <  1:1476788/139‑1 (MQ=255)
  ttGATTCGCGACCTTTTCTGCATCTGGTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATa                                                                                                                       <  1:739886/139‑1 (MQ=255)
                     gCATCTGGTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAg                                                                                                    <  2:540118/139‑1 (MQ=255)
                           ggTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATAcgc                                                                                                                    <  2:526855/117‑1 (MQ=255)
                           ggTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATAcgc                                                                                                                    >  1:526855/1‑117 (MQ=255)
                           ggTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTaa                                                                                              >  1:1268780/1‑139 (MQ=255)
                                tggtggAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAg                                                                                                    >  2:1466564/1‑128 (MQ=255)
                                tggtggAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAg                                                                                                    <  1:1466564/128‑1 (MQ=255)
                                tggtggAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTaaaa                                                                                            <  1:1337558/136‑1 (MQ=255)
                                tggtggAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTaaaa                                                                                            >  2:1337558/1‑136 (MQ=255)
                                tggtggAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAggg                                                                                         >  2:1098747/1‑139 (MQ=255)
                                       aCTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTaa                                                                                  >  2:1430698/1‑139 (MQ=255)
                                           ccAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACt                                                                              >  1:1206587/1‑139 (MQ=255)
                                                             acacGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTc                                                            <  1:791392/139‑1 (MQ=255)
                                                                   tctctatGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGcc                                                      <  2:395343/139‑1 (MQ=255)
                                                                      ctctGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATAcgc                                                                                                                    >  2:602686/1‑74 (MQ=255)
                                                                      ctctGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATAcgc                                                                                                                    <  1:602686/74‑1 (MQ=255)
                                                                           tCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTa                                              >  2:637728/1‑139 (MQ=255)
                                                                               tgggCAGTGAGGTGCTGGCTGGGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCa                                          >  1:883408/3‑139 (MQ=255)
                                                                               tAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATAcg                                                                                                                     >  2:718199/1‑64 (MQ=255)
                                                                               tAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATAcg                                                                                                                     <  1:718199/64‑1 (MQ=255)
                                                                               tAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCa                                          >  2:1388758/1‑139 (MQ=255)
                                                                                   cAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTggcg                                                                                                          <  2:1181974/71‑1 (MQ=255)
                                                                                   cAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTggcg                                                                                                          >  1:1181974/1‑71 (MQ=255)
                                                                                     gTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCAt                                                                                                                            >  1:305128/1‑51 (MQ=255)
                                                                                     gTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCAt                                                                                                                            <  2:305128/51‑1 (MQ=255)
                                                                                     gTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTgg                                                                                                            >  2:1145264/1‑67 (MQ=255)
                                                                                     gTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTgg                                                                                                            <  1:1145264/67‑1 (MQ=255)
                                                                                     gTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCa                                                                                                  <  1:888586/77‑1 (MQ=255)
                                                                                     gTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCa                                                                                                  >  2:888586/1‑77 (MQ=255)
                                                                                     gTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTaaa                                                                                             >  1:549667/1‑82 (MQ=255)
                                                                                     gTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTaaa                                                                                             <  2:549667/82‑1 (MQ=255)
                                                                                     gTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCg                                                                 <  2:1463781/110‑1 (MQ=255)
                                                                                     gTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCg                                                                 >  1:1463781/1‑110 (MQ=255)
                                                                                     gTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTg                                    >  1:69468/1‑139 (MQ=255)
                                                                                     gTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTg                                    >  2:1006517/1‑139 (MQ=255)
                                                                                      tGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAg                                                                                                    <  1:1308237/74‑1 (MQ=255)
                                                                                      tGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAg                                                                                                    >  2:1308237/1‑74 (MQ=255)
                                                                                      tGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTgg                                   >  1:593113/1‑139 (MQ=255)
                                                                                       gAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCAt                                                          <  2:256778/115‑1 (MQ=255)
                                                                                       gAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCAt                                                          >  1:256778/1‑115 (MQ=255)
                                                                                       gAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCACAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGa                                  <  1:1430698/139‑1 (MQ=255)
                                                                                                gctgTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCTATACTGGCGGCGTAGCAGTAAAAGGGTCTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACg                         >  1:372337/1‑139 (MQ=255)
                                                                                                gctgTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAg                                                                                                    >  1:124860/1‑64 (MQ=255)
                                                                                                gctgTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAg                                                                                                    <  2:124860/64‑1 (MQ=255)
                                                                                                    tgtACATTAATTTGATTGTTTGCCCACGCTTGGCAt                                                                                                                            >  1:1373406/1‑36 (MQ=255)
                                                                                                    tgtACATTAATTTGATTGTTTGCCCACGCTTGGCAt                                                                                                                            <  2:1373406/36‑1 (MQ=255)
                                                                                                    tgtACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGtt                     <  1:637728/139‑1 (MQ=255)
                                                                                                     gtACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGcc                                                      >  2:1052448/1‑105 (MQ=255)
                                                                                                     gtACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGcc                                                      <  1:1052448/105‑1 (MQ=255)
                                                                                                          ttAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTccc               >  1:387087/1‑139 (MQ=255)
                                                                                                              tttGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGc                                                                            <  2:1116238/74‑1 (MQ=255)
                                                                                                              tttGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGc                                                                            >  1:1116238/1‑74 (MQ=255)
                                                                                                                tGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAg                                                                                                    >  1:461703/1‑48 (MQ=255)
                                                                                                                tGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAg                                                                                                    <  2:461703/48‑1 (MQ=255)
                                                                                                                tGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTAtt         >  2:1364239/1‑139 (MQ=255)
                                                                                                                     gTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCa    <  2:387087/139‑1 (MQ=255)
                                                                                                                       ttGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGa  <  1:1606/139‑1 (MQ=255)
                                                                                                                       ttGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGa  <  1:1006517/139‑1 (MQ=255)
                                                                                                                            cACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCa                            <  2:1033885/108‑1 (MQ=255)
                                                                                                                            cACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCa                            >  1:1033885/1‑108 (MQ=255)
                                                                                                                                |                                                                                                                                 
CTTTGATTCGCGACCTTTTCTGCATCTGGTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGA  >  NC_000913/155807‑156064

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑