Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,548,360	C→T	D320D (GAC→GAT)	fdnG →	formate dehydrogenase‑N, alpha subunit, nitrate‑inducible

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,548,360	0	C	T	100.0%	151.6 / NA	47	D320D (GAC→GAT)	fdnG	formate dehydrogenase‑N, alpha subunit, nitrate‑inducible
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/22);  total (25/22)

TACGGACATTACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGACGGTCTGT‑‑TCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGACTCATCCGCGCT  >  NC_000913/1548234‑1548475                                                                                                                               |                                                                                                                      tACGGACATTACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATgg                                                                                                                     >  1:1289114/1‑129 (MQ=255) tACGGACATTACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATgg                                                                                                                     <  2:1289114/129‑1 (MQ=255)      aCATTACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGT‑‑TCAGCGGCTa                                                                                                    >  1:1000915/1‑139 (MQ=255)         ttACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGT‑‑TCAGCGGCTacga                                                                                                 >  2:1261607/1‑139 (MQ=255)          tACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGT‑‑TCAGCGGCTacgac                                                                                                >  2:28616/1‑139 (MQ=255)           aCGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGT‑‑TCAGCGGCTacgacg                                                                                               <  2:1458410/139‑1 (MQ=255)              ttCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCTCAGCGGCTacgacg                                                                                               >  2:476103/1‑138 (MQ=255)              ttCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCTCAGCGGCTacgacg                                                                                               <  1:476103/138‑1 (MQ=255)                  tGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGT‑‑TCAGCGGCTACGACGCTGaaaa                                                                                        >  2:717813/1‑139 (MQ=255)                        gCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGT‑‑TCAGCGGCTACGACGCTGAAAAACGTCa                                                                                  >  1:297647/1‑139 (MQ=255)                         cGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGT‑‑TCAGCGGCTACGACGCTGAAAAACGTCaa                                                                                 <  1:717813/139‑1 (MQ=255)                              tGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGT‑‑TCAGCGGCTACGACGCTGAAAAACGTCAATACGa                                                                            >  2:318302/1‑139 (MQ=255)                                     ccTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGT‑‑TCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCg                                                                     <  1:318302/139‑1 (MQ=255)                                             aaaaCAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGT‑‑TCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGaa                                                             <  1:28616/139‑1 (MQ=255)                                               aacaacAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGT‑‑TCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACt                                                           >  1:219569/1‑139 (MQ=255)                                                    caaAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGT‑‑TCAg                                                                                                          <  1:310995/87‑1 (MQ=255)                                                    caaAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGT‑‑TCAg                                                                                                          >  2:310995/1‑87 (MQ=255)                                                              gCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGT‑‑TCAg                                                                                                          <  2:82391/77‑1 (MQ=255)                                                              gCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGT‑‑TCAg                                                                                                          >  1:82391/1‑77 (MQ=255)                                                                            gCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGT‑‑TCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGaaa                              >  1:36479/1‑139 (MQ=255)                                                                               ttACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGT‑‑TCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTc                                                                   <  1:145020/99‑1 (MQ=255)                                                                               ttACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGT‑‑TCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTc                                                                   >  2:145020/1‑99 (MQ=255)                                                                                tACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGT‑‑TCAGCGGCTACGACGCTGAAAAAc                                                                                      >  2:1269061/1‑79 (MQ=255)                                                                                tACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGT‑‑TCAGCGGCTACGACGCTGAAAAAc                                                                                      <  1:1269061/79‑1 (MQ=255)                                                                                tACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGT‑‑TCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAAcgcg                          >  2:867113/1‑139 (MQ=255)                                                                                       aCGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGT‑‑TCAGCGGCTa                                                                                                    <  2:1170613/58‑1 (MQ=255)                                                                                       aCGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGT‑‑TCAGCGGCTa                                                                                                    >  1:1170613/1‑58 (MQ=255)                                                                                       aCGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGT‑‑TCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAac                   >  2:1104939/1‑139 (MQ=255)                                                                                       aCGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGT‑‑TCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAac                   <  2:297647/139‑1 (MQ=255)                                                                                        cGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGT‑‑TCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAcaca                  <  2:1000915/139‑1 (MQ=255)                                                                                          ccAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGT‑‑TCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACt                >  1:1456734/1‑139 (MQ=255)                                                                                             gcttgctgGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGT‑‑TCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGCCCTgg                                                               <  2:1354818/86‑1 (MQ=255)                                                                                             gCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGT‑‑TCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTgg                                                               >  1:1354818/1‑89 (MQ=255)                                                                                              ccTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGT‑‑TCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTgg                                                               >  2:97336/1‑88 (MQ=255)                                                                                              ccTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGT‑‑TCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTgg                                                               <  1:97336/88‑1 (MQ=255)                                                                                              ccTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGT‑‑TCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGACt            <  2:36479/139‑1 (MQ=255)                                                                                                  ctgGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGT‑‑TCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGACTCATc        >  2:758187/1‑139 (MQ=255)                                                                                                  ctgATGCGTGATGATTTTGCTTTCGAAGATGGTCTGT‑‑TCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGACTCATc        >  2:641213/1‑139 (MQ=255)                                                                                                   tgGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGT‑‑TCAGCGGCTa                                                                                                    >  2:749731/1‑46 (MQ=255)                                                                                                   tgGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGT‑‑TCAGCGGCTa                                                                                                    <  1:749731/46‑1 (MQ=255)                                                                                                     gTGCGTGATGATTTTGCTTTCGAAGATGGTCTGT‑‑TCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAACCACTGACTCATCcgc     >  2:1392198/1‑139 (MQ=255)                                                                                                      tGCGTGATGATTTTGCTTTCGAAGATGGTCTGT‑‑TCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAACCACTGACTCATCcgcg    <  1:1392198/139‑1 (MQ=255)                                                                                                      tGCGTGATGATTTTGCTTTCGAAGATGGTCTGT‑‑TCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGACTCATCcgcg    <  2:219569/139‑1 (MQ=255)                                                                                                      tGCGTGATGATTTTGCTTTCGAAGATGGTCTGT‑‑TCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGACTCATCcgcg    <  2:1220044/139‑1 (MQ=255)                                                                                                       gCGTGATGATTTTGCTTTCGAAGATGGTCTGT‑‑TCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAATGGCTATGCGAAACGCGATGAAACACTGACt            >  2:493307/1‑130 (MQ=255)                                                                                                       gCGTGATGATTTTGCTTTCGAAGATGGTCTGT‑‑TCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAATGGCTATGCGAAACGCGATGAAACACTGACt            <  1:493307/130‑1 (MQ=255)                                                                                                        cGTGATGATTTTGCTTTCGAAGATGGTCTGT‑‑TCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGACTCATCCGCGCt  <  2:1456734/139‑1 (MQ=255)                                                                                                                               |                                                                                                                      TACGGACATTACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGACGGTCTGT‑‑TCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGACTCATCCGCGCT  >  NC_000913/1548234‑1548475

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑