Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,598,617	C→T	*531* (TAG→TAA)	lsrK ←	autoinducer‑2 (AI‑2) kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,598,617	0	C	T	100.0%	233.8 / NA	72	*531* (TAG→TAA)	lsrK	autoinducer‑2 (AI‑2) kinase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (38/34);  total (38/34)

TGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTCCGGGTCTGGT  >  NC_000913/1598494‑1598743                                                                                                                            |                                                                                                                               tGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAATTATAACCCAGGCGCt                                                                                                                 <  2:134464/139‑1 (MQ=255)   gTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAATTATAACCCAGGCGCttt                                                                                                               >  2:958600/1‑139 (MQ=255)        ggAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTAGCTGTCAATGAAATTATAACCCAGGCGCTTTCCATa                                                                                                          >  1:625517/1‑139 (MQ=255)          aaCGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAac                                                                                                        >  1:398263/1‑139 (MQ=255)             gATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAacgac                                                                                                     >  1:1288393/1‑139 (MQ=255)                  aTCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTAGCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGAcgtcgt                                                                                                <  2:625517/139‑1 (MQ=255)                           cTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATg                                                                                       >  1:731708/1‑139 (MQ=255)                             ggCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGAt                                                                                     >  1:491406/1‑139 (MQ=255)                                              ctctAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAATTATAACCCAGGCGCTTTCCATa                                                                                                          <  2:459863/101‑1 (MQ=255)                                              ctctAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAATTATAACCCAGGCGCTTTCCATa                                                                                                          >  1:459863/1‑101 (MQ=255)                                              ctctAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGa                                                                    <  2:255437/139‑1 (MQ=255)                                                ctAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAGTTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATc                                                                  <  1:958600/139‑1 (MQ=255)                                                        agGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATaaa                                                          >  2:534609/1‑139 (MQ=255)                                                        agGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATaaa                                                          >  1:88106/1‑139 (MQ=255)                                                        agGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATaaa                                                          >  2:1418733/1‑139 (MQ=255)                                                        agGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATaaa                                                          <  1:1418733/139‑1 (MQ=255)                                                         gggAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCt                                                                      <  2:651626/126‑1 (MQ=255)                                                         gggAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCt                                                                      >  1:651626/1‑126 (MQ=255)                                                         gggAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAAc                                                         >  1:69601/1‑139 (MQ=255)                                                            aaaTAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAActgc                                                      <  2:398263/139‑1 (MQ=255)                                                                    aTAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACt                                              >  2:1094000/1‑139 (MQ=255)                                                                     tAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGa                                                                    <  2:632570/116‑1 (MQ=255)                                                                     tAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGa                                                                    >  1:632570/1‑116 (MQ=255)                                                                          ataCTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAATTATAACCCAGGCGCt                                                                                                                 <  1:630899/66‑1 (MQ=255)                                                                          ataCTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAATTATAACCCAGGCGCt                                                                                                                 >  2:630899/1‑66 (MQ=255)                                                                           taCTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGAcgtcgt                                                                                                <  2:1457409/82‑1 (MQ=255)                                                                           taCTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGAcgtcgt                                                                                                >  1:1457409/1‑82 (MQ=255)                                                                           taCTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATcgcg                                       >  2:1417381/1‑139 (MQ=255)                                                                             cTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAATTATAACCCAGgcgc                                                                                                                  <  2:1473747/62‑1 (MQ=255)                                                                             cTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAATTATAACCCAGgcgc                                                                                                                  >  1:1473747/1‑62 (MQ=255)                                                                             cTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAacga                                                                                                      <  1:496442/74‑1 (MQ=255)                                                                             cTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAacga                                                                                                      >  2:496442/1‑74 (MQ=255)                                                                             cTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGACgtcgtc                                                                                               >  1:849357/1‑81 (MQ=255)                                                                             cTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGACgtcgtc                                                                                               <  2:849357/81‑1 (MQ=255)                                                                             cTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAAcc                                                                                >  2:108176/1‑96 (MQ=255)                                                                             cTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAAcc                                                                                <  1:108176/96‑1 (MQ=255)                                                                             cTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGa                                                                    <  2:727808/108‑1 (MQ=255)                                                                             cTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGa                                                                    >  1:727808/1‑108 (MQ=255)                                                                             cTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTg                                     >  1:1313836/1‑139 (MQ=255)                                                                               cAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGaa                                   <  2:1288393/139‑1 (MQ=255)                                                                                aaaaaCTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAAt                                  <  1:1417381/139‑1 (MQ=255)                                                                                  aaccTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAActgc                                                      <  2:820658/114‑1 (MQ=255)                                                                                  aaaCTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAATTATAACCCAGgcgc                                                                                                                  >  1:1460377/1‑57 (MQ=255)                                                                                  aaaCTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAATTATAACCCAGgcgc                                                                                                                  <  2:1460377/57‑1 (MQ=255)                                                                                  aaaCTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAActgc                                                      >  1:820658/1‑117 (MQ=255)                                                                                          aGCCACGGTCATCATGATGTGGCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGc                                                                             <  1:103750/86‑1 (MQ=255)                                                                                          aGCCACGGTCATCATGATGTGGCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGc                                                                             >  2:103750/1‑86 (MQ=255)                                                                                                ggTCATCATGATGTGGCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAActgc                                                      <  1:875911/103‑1 (MQ=255)                                                                                                ggTCATCATGATGTGGCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAActgc                                                      >  2:875911/1‑103 (MQ=255)                                                                                                  tcatcaTGATGTGGCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCt                >  2:1455885/1‑139 (MQ=255)                                                                                                      catGATGTGGCTGTCAATGAAATTATAACCCAGGCTCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAGGTTCATGCTTTTc            <  2:1465195/139‑1 (MQ=255)                                                                                                      catGATGTGGCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAGGTTCATGCTTTTc            <  2:491406/139‑1 (MQ=255)                                                                                                            gtgGCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAac                                                                                                        >  2:190264/1‑41 (MQ=255)                                                                                                            gtgGCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAac                                                                                                        <  1:190264/41‑1 (MQ=255)                                                                                                            gtgGCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGAcgtcgt                                                                                                <  1:415072/49‑1 (MQ=255)                                                                                                            gtgGCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGAcgtcgt                                                                                                >  2:415072/1‑49 (MQ=255)                                                                                                              ggCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCTCTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTCCGGGTCTg    <  1:1041018/139‑1 (MQ=255)                                                                                                               gCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGAcgtcgt                                                                                                <  1:919871/46‑1 (MQ=255)                                                                                                               gCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGAcgtcgt                                                                                                >  2:919871/1‑46 (MQ=255)                                                                                                               gCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTc            >  1:854993/1‑130 (MQ=255)                                                                                                               gCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTc            <  2:854993/130‑1 (MQ=255)                                                                                                                cTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGAcgtcgt                                                                                                >  2:496937/1‑45 (MQ=255)                                                                                                                cTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGAcgtcgt                                                                                                <  1:496937/45‑1 (MQ=255)                                                                                                                cTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGAcgtcgt                                                                                                <  1:1394112/45‑1 (MQ=255)                                                                                                                cTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGAcgtcgt                                                                                                <  1:126638/45‑1 (MQ=255)                                                                                                                cTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGAcgtcgt                                                                                                >  2:1394112/1‑45 (MQ=255)                                                                                                                cTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGAcgtcgt                                                                                                >  2:126638/1‑45 (MQ=255)                                                                                                                cTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTCCGGGTCTGgt  >  2:265268/1‑139 (MQ=255)                                                                                                                   tCAATGAAATTATAACCCAGGCGCTTTCCATAACTACGTCGTCAGt                                                                                            <  1:731588/46‑1 (MQ=38)                                                                                                                   tCAATGAAATTATAACCCAGGCGCTTTCCATAACTACGTCGTCAGt                                                                                            >  2:731588/1‑46 (MQ=255)                                                                                                                     aaTGAAATTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGa                                                                    >  2:356815/1‑68 (MQ=255)                                                                                                                     aaTGAAATTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGa                                                                    <  1:356815/68‑1 (MQ=255)                                                                                                                            |                                                                                                                               TGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTCCGGGTCTGGT  >  NC_000913/1598494‑1598743

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑