Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,618,218	G→A	*397* (TAG→TAA)	ydeA →	arabinose efflux transporter, arabinose‑inducible

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,618,218	0	G	A	100.0%	174.3 / NA	54	*397* (TAG→TAA)	ydeA	arabinose efflux transporter, arabinose‑inducible
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/26);  total (28/26)

TAATCAGGTGAGTTTGCACTGGTCAATGTCGATGATTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCATCAGACGGGAGATCGCTTCAAT  >  NC_000913/1618086‑1618341                                                                                                                                     |                                                                                                                            taATCAGGTGAGTTTGCACTGGTCAATGTCGATGATTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACAATCGAAGAACAGACGCAATAATTGaaa                                                                                                                       >  1:937700/1‑139 (MQ=255)   aTCAGGTGAGTTTGCACTGGTCAATGTCGATGATTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAgg                                                                                                                     >  1:751718/1‑139 (MQ=255)    tCAGGTGAGTTTGCACTGGTCAATGTCGATGATTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGc                                                                                                                    >  1:1126957/1‑139 (MQ=255)    tCAGGTGAGTTTGCACTGGTCAATGTCGATGATTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGc                                                                                                                    >  2:175675/1‑139 (MQ=255)          gAGTTTGCACTGGTCAATGTCGATGATTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTc                                                                                                              >  1:828792/1‑139 (MQ=255)             tttGCACTGGTCAATGTCGATGATTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCggg                                                                                                           <  1:1041466/139‑1 (MQ=255)               tGCACTGGTCAATGTCGATGATTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGcc                                                                                                         <  2:828792/139‑1 (MQ=255)                                atgatTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGtt                                                                                        >  1:251864/1‑139 (MQ=255)                                 tgatTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCAtt                                                                                                               >  2:1065954/1‑115 (MQ=255)                                 tgatTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCAtt                                                                                                               <  1:1065954/115‑1 (MQ=255)                                    ttGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTaa                                                                                    <  1:175675/139‑1 (MQ=255)                                       gTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGa                                                                                 >  1:650381/1‑139 (MQ=255)                                         tATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCttttt                                                                                                    >  2:500081/1‑118 (MQ=255)                                         tATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCttttt                                                                                                    <  1:500081/118‑1 (MQ=255)                                             tgGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCa                                                                           >  1:242343/1‑139 (MQ=255)                                               ggCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGa                                                                                                                         <  1:1173877/91‑1 (MQ=255)                                               ggCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGa                                                                                                                         >  2:1173877/1‑91 (MQ=255)                                               ggCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGcc                                                                                                         <  2:85239/107‑1 (MQ=255)                                               ggCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGcc                                                                                                         >  1:85239/1‑107 (MQ=255)                                               ggCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAgg                                                                         >  2:1158074/1‑139 (MQ=255)                                                      tGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGt                                                                  <  2:650381/139‑1 (MQ=255)                                                      tGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGt                                                                  <  2:251864/139‑1 (MQ=255)                                                              tttGCCTCGTTAATTTTGTCAATGATTATATTTCGCCGCTGGCCAGTTACACTCGAAGAACTGACACAATAATTGAAAGGCCCATTCGGGTCTTTTTTAATGCTACGTTTTAATGAGTCCCAGGATGCCGTtaagaata                                                          <  1:931305/139‑1 (MQ=255)                                                                     cgTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGca                                                   <  1:1158074/139‑1 (MQ=255)                                                                     cgTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGca                                                   <  1:596153/139‑1 (MQ=255)                                                                       ttAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGcaca                                                 <  2:1126957/139‑1 (MQ=255)                                                                                              tCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGa                                                                                                                         >  2:1224723/1‑44 (MQ=255)                                                                                              tCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGa                                                                                                                         <  1:1224723/44‑1 (MQ=255)                                                                                              tCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATcc                          >  1:584124/1‑139 (MQ=255)                                                                                               cgccgcTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCttt                                                                                                      >  1:579342/1‑62 (MQ=255)                                                                                               cgccgcTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCttt                                                                                                      <  2:579342/62‑1 (MQ=255)                                                                                               cgccgcTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGt                                                                  >  2:968246/1‑98 (MQ=255)                                                                                               cgccgcTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGt                                                                  <  1:968246/98‑1 (MQ=255)                                                                                               cgccgcTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTtaataata                                                          >  1:1283987/1‑106 (MQ=255)                                                                                               cgccgcTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTtaataata                                                          <  2:1283987/106‑1 (MQ=255)                                                                                                 ccgcTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCAt                       <  2:937700/139‑1 (MQ=255)                                                                                                 ccgcTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCAt                       <  2:660895/139‑1 (MQ=255)                                                                                                  cgcTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCATc                      >  1:467640/1‑139 (MQ=255)                                                                                                       gCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGt                                                                  >  2:110937/1‑90 (MQ=255)                                                                                                       gCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGt                                                                  <  1:110937/90‑1 (MQ=255)                                                                                                                 cTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGt                                                                                             >  1:600494/1‑53 (MQ=255)                                                                                                                 cTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGt                                                                                             <  2:600494/53‑1 (MQ=255)                                                                                                                 cTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGca                                                   <  2:133751/95‑1 (MQ=255)                                                                                                                 cTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGca                                                   >  1:133751/1‑95 (MQ=255)                                                                                                                 cTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCATCAGACGGGAGATCGCt       >  1:791209/1‑139 (MQ=255)                                                                                                                      agaaCAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCATCAGACGGGAGATCGCTTCAAt  >  1:637992/1‑139 (MQ=255)                                                                                                                         aCAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAgg                                                                         >  2:1225771/1‑65 (MQ=255)                                                                                                                         aCAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAgg                                                                         <  1:1225771/65‑1 (MQ=255)                                                                                                                             aCGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATg                                                                                  <  2:1057586/52‑1 (MQ=255)                                                                                                                             aCGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATg                                                                                  >  1:1057586/1‑52 (MQ=255)                                                                                                                              cGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTtaataata                                                          >  2:1216148/1‑75 (MQ=255)                                                                                                                              cGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTtaataata                                                          <  1:1216148/75‑1 (MQ=255)                                                                                                                                  aTAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGc                                                    <  2:1292550/77‑1 (MQ=255)                                                                                                                                  aTAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGc                                                    >  1:1292550/1‑77 (MQ=255)                                                                                                                                     |                                                                                                                            TAATCAGGTGAGTTTGCACTGGTCAATGTCGATGATTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCATCAGACGGGAGATCGCTTCAAT  >  NC_000913/1618086‑1618341

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑