Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,621,542	G→A	D71N (GAC→AAC)	ydeE →	putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,621,542	0	G	A	100.0%	151.8 / NA	47	D71N (GAC→AAC)	ydeE	putative transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/24);  total (23/24)

CTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCT‑‑CACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCGACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGCTCTTTTTTGCCCTCATTAACTGCGCCTATTCTG  >  NC_000913/1621410‑1621665                                                                                                                                       |                                                                                                                            cTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCT‑‑CACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGa                                                                                                                       <  1:1409834/139‑1 (MQ=255) cTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCT‑‑CACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGa                                                                                                                       <  2:614168/139‑1 (MQ=255)  tGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCT‑‑CACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGaa                                                                                                                      <  2:682349/139‑1 (MQ=255)              ccATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCT‑‑CACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCtat                                                                                                               <  2:388701/134‑1 (MQ=255)              ccATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCT‑‑CACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCtat                                                                                                               >  1:388701/1‑134 (MQ=255)                 tttACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCT‑‑CACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCt                                                                                                                 <  2:127360/129‑1 (MQ=255)                 tttACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCT‑‑CACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCt                                                                                                                 >  1:127360/1‑129 (MQ=255)                                aGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCT‑‑CACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCtt                                                                                        <  2:1288024/139‑1 (MQ=255)                                      gCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCT‑‑CACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAg                                                                                  <  1:558624/139‑1 (MQ=255)                                            gtgtCGATCTAATCGGTTATGCGATGACAATTGCGCT‑‑CACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAg                                                                                                                        <  1:51116/95‑1 (MQ=255)                                            gtgtCGATCTAATCGGTTATGCGATGACAATTGCGCT‑‑CACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAg                                                                                                                        >  2:51116/1‑95 (MQ=255)                                            gtgtCGATCTAATCGGTTATGCGATGACAATTGCGCT‑‑CACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGttt                                                                            >  1:1037020/1‑139 (MQ=255)                                                    cTAATCGGTTATGCGATGACAATTGCGCTCACACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGc                                                                      <  1:583934/139‑1 (MQ=255)                                                     tAATCGGTTATGCGATGACAATTGCGCT‑‑CACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCt                                                                                                                 >  2:368510/1‑93 (MQ=255)                                                     tAATCGGTTATGCGATGACAATTGCGCT‑‑CACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCt                                                                                                                 <  1:368510/93‑1 (MQ=255)                                                       aTCGGTTATGCGATGACAATTGCGCT‑‑CACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTa                                                                 >  2:767900/1‑139 (MQ=255)                                                             tATGCGATGACAATTGCGCT‑‑CACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGa                                                                                                                       <  2:1010710/79‑1 (MQ=255)                                                             tATGCGATGACAATTGCGCT‑‑CACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGa                                                                                                                       >  1:1010710/1‑79 (MQ=255)                                                             tATGCGATGACAATTGCGCT‑‑CACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCT‑GCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAg                                                                                  <  2:1384748/115‑1 (MQ=255)                                                             tATGCGATGACAATTGCGCT‑‑CACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCT‑GCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAg                                                                                  >  1:1384748/1‑115 (MQ=255)                                                               tGCGATGACAATTGCGCT‑‑CACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTg                                                         >  2:1077513/1‑139 (MQ=255)                                                                 cGATGACAATTGCGCT‑‑CACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACt                                                               <  1:1291922/131‑1 (MQ=255)                                                                 cGATGACAATTGCGCT‑‑CACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACt                                                               >  2:1291922/1‑131 (MQ=255)                                                                 cGATGACAATTGCGCT‑‑CACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGaa                                                       <  2:1037020/139‑1 (MQ=255)                                                                       cAATTGCGCT‑‑CACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGt                                                 <  1:767900/139‑1 (MQ=255)                                                                             cgcT‑‑CACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCt                                           >  1:1233231/1‑139 (MQ=255)                                                                                       aTTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGc                                   <  1:1105163/139‑1 (MQ=255)                                                                                             gtcgtTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGCTCtttt                             <  2:1233231/139‑1 (MQ=255)                                                                                              tcgtTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCtat                                                                                                               >  2:1320036/1‑56 (MQ=255)                                                                                              tcgtTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCtat                                                                                                               <  1:1320036/56‑1 (MQ=255)                                                                                              tcgtTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGCTCttttt                            >  2:1350934/1‑139 (MQ=255)                                                                                              tcgtTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGCTCttttt                            >  1:1017311/1‑139 (MQ=255)                                                                                              tcgtTTTTAGCCTCGGTTTTGGCATCCTGGCGGATAAGTTCAACAAGaa                                                                                                                      >  1:165340/1‑49 (MQ=255)                                                                                              tcgtTTTTAGCCTCGGTTTTGGCATCCTGGCGGATAAGTTCAACAAGaa                                                                                                                      <  2:165340/49‑1 (MQ=255)                                                                                                gtTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTa                                                                          >  1:175014/1‑91 (MQ=255)                                                                                                gtTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTa                                                                          <  2:175014/91‑1 (MQ=255)                                                                                                gtTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGCTCTTTTTTg                          >  1:739506/1‑139 (MQ=255)                                                                                                gtTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGAAGCTGGTTGTGCTCTTTTTTg                          >  1:910829/1‑139 (MQ=255)                                                                                                  ttttAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGCTCTTTTTTGcc                        <  1:1077513/139‑1 (MQ=255)                                                                                                           cGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACg                                                                                                                   >  2:1071966/1‑39 (MQ=255)                                                                                                           cGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACg                                                                                                                   <  1:1071966/39‑1 (MQ=38)                                                                                                           cGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTa                                                                          <  2:14403/80‑1 (MQ=255)                                                                                                           cGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTa                                                                          >  1:14403/1‑80 (MQ=255)                                                                                                           cGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGCTCTTTTTTGCCCTCATTAAc               >  2:784303/1‑139 (MQ=255)                                                                                                           cGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGCTCTTTTTTGCCCTCATTAAc               >  2:853128/1‑139 (MQ=255)                                                                                                                     aTCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGCTCTTTTTTGCCCTCATTATCTGCGCCTAtt     <  2:910829/139‑1 (MQ=255)                                                                                                                        cTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGCTCTTTTTTGCCCTCATTAACTGCGCCTATTCTg  >  1:349102/1‑139 (MQ=255)                                                                                                                                       |                                                                                                                            CTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCT‑‑CACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCGACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGCTCTTTTTTGCCCTCATTAACTGCGCCTATTCTG  >  NC_000913/1621410‑1621665

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑