Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,655,057	T→C	T29A (ACG→GCG)	rspA ←	bifunctional D‑altronate/D‑mannonate dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,655,057	0	T	C	100.0%	206.7 / NA	59	T29A (ACG→GCG)	rspA	bifunctional D‑altronate/D‑mannonate dehydratase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (32/27);  total (32/27)

ACCTTTATAGAAAAACTGCCAGATATCTTCGATACGGTGCGCATCGCGACCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGTAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCTACGCAACTG  >  NC_000913/1654923‑1655190                                                                                                                                       |                                                                                                                                      aCCTTTATAGAAAAACTGCCAGATATCTTCGATACGGTGCGCATCGCGACCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATa                                                                                                                                   <  2:225693/139‑1 (MQ=255) aCCTTTATAGAAAAACTGCCAGATATCTTCGATACGGTGCGCATCGCGACCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATa                                                                                                                                   <  1:783367/139‑1 (MQ=255)   cTTTATAGAAAAACTGCCAGATATCTTCGATACGGTGCGCATCGCGACCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATAcc                                                                                                                                 >  2:1107506/1‑139 (MQ=255)               aCTGCCAGATATCTTCGATACGGTGCGCATCGCGACCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAgtggt                                                                                                                     <  2:440174/139‑1 (MQ=255)                         atCTTCGATACGGTGCGCATCGCGACCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAAt                                                                                                           <  1:342553/139‑1 (MQ=255)                             tCGATACGGTGCGCATCGCGACCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGa                                                                                                       >  1:713689/1‑139 (MQ=255)                                aTACGGTGCGCATCGCGACCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGa                                                                                                    >  2:1168088/1‑139 (MQ=255)                                    ggTGCGCATCGCGACCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAAtt                                                                                                >  2:549862/1‑139 (MQ=255)                                     gTGCGCATCGCGACAAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTa                                                                                               >  2:893424/1‑139 (MQ=255)                                      tGCGCATCGCGACCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTAc                                                                                              >  2:422179/1‑139 (MQ=255)                                        cgcATCGCGACCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGc                                                                                            >  1:975724/1‑139 (MQ=255)                                           aTCGCGACCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGcccc                                                                                         >  1:1245038/1‑139 (MQ=255)                                             cgcgACCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCgg                                                                                       >  1:573427/1‑139 (MQ=255)                                              gcgACCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGa                                                                                      <  2:713689/139‑1 (MQ=255)                                              gcgACCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGa                                                                                      >  1:1403057/1‑139 (MQ=255)                                                    aaTAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTa                                                                                <  1:1168088/139‑1 (MQ=255)                                                        aGCGGCGGACAAAGGTGATCCTGCAAATAAGATGCCACGGAAAGCGCACGTCCATTGAGGGTAGCATCCCCAAGGCCCGCAATACCGTCGTCAGTAGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACaa                                                                            <  1:699080/139‑1 (MQ=255)                                                            gCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCt                                                                                                                            <  1:242982/87‑1 (MQ=255)                                                            gCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCt                                                                                                                            >  2:242982/1‑87 (MQ=255)                                                            gCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAAc                                                                        >  1:912505/1‑139 (MQ=255)                                                              ggACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACtt                                                                      <  1:1163087/139‑1 (MQ=255)                                                               gACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCTCCGGACAGGTAACAAAAACTTc                                                                     >  2:323472/1‑139 (MQ=255)                                                                 cAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAg                                                                   <  2:1245038/139‑1 (MQ=255)                                                                    aGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACaa                                                                            <  2:414940/127‑1 (MQ=255)                                                                    aGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACaa                                                                            >  1:414940/1‑127 (MQ=255)                                                                    aGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCt                                                                <  1:893424/139‑1 (MQ=255)                                                                       tGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTa                                                             >  1:960438/1‑139 (MQ=255)                                                                         aTCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACTAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACg                                                           <  2:960438/139‑1 (MQ=255)                                                                         aTCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACg                                                           >  2:973087/1‑139 (MQ=255)                                                                          tCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGa                                                          >  2:297679/1‑139 (MQ=255)                                                                                aaaTAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGcc                                                                                           >  1:979480/1‑100 (MQ=255)                                                                                aaaTAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGcc                                                                                           <  2:979480/100‑1 (MQ=255)                                                                                      gagGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCg                                              <  1:1166021/139‑1 (MQ=255)                                                                                      gagGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCg                                              <  2:573427/139‑1 (MQ=255)                                                                                         gCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAAtt                                                                                                <  2:668780/86‑1 (MQ=255)                                                                                         gCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAAtt                                                                                                >  1:668780/1‑86 (MQ=255)                                                                                           cACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTcc                                         >  2:1204128/1‑139 (MQ=255)                                                                                              ggAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTg                                      >  2:873876/1‑139 (MQ=255)                                                                                                aaaGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTCACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCa                                    <  2:912505/139‑1 (MQ=255)                                                                                                           ccATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGt                         <  1:404591/139‑1 (MQ=255)                                                                                                             aTTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGa                       <  2:975724/139‑1 (MQ=255)                                                                                                             aTTGAGGGTGGAATCCCACAGGCCTGCAATACGTACCTCAGTGGTGAGTTTTAATGTGACGAAATGACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGa                       <  1:1107506/139‑1 (MQ=255)                                                                                                                     tGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGaa               <  1:973087/139‑1 (MQ=255)                                                                                                                     tGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGaa               <  2:1091008/139‑1 (MQ=255)                                                                                                                     tGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGaa               >  1:1091008/1‑139 (MQ=255)                                                                                                                     tGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCACCGGACAGGTAACAAAAACTTCAGCCTTTACGGTCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGaa               >  1:955928/1‑139 (MQ=255)                                                                                                                      ggCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGaaa              >  1:746970/1‑139 (MQ=255)                                                                                                                      ggCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGaaa              >  1:619225/1‑139 (MQ=255)                                                                                                                      ggCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGaaa              >  1:164294/1‑139 (MQ=255)                                                                                                                       gCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAAtt                                                                                                >  1:835864/1‑56 (MQ=255)                                                                                                                       gCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAAtt                                                                                                <  2:835864/56‑1 (MQ=255)                                                                                                                       gCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCTCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAAt             <  1:323472/139‑1 (MQ=255)                                                                                                                       gCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAAt             >  2:632524/1‑139 (MQ=255)                                                                                                                           ccccAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGa                                                                                                       >  2:768744/1‑45 (MQ=255)                                                                                                                           ccccAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGa                                                                                                       <  1:768744/45‑1 (MQ=255)                                                                                                                            cccAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCTACg        >  2:456424/1‑139 (MQ=255)                                                                                                                             ccAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCTa          <  1:1416852/136‑1 (MQ=255)                                                                                                                             ccAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCTa          >  2:1416852/1‑136 (MQ=255)                                                                                                                                  gCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCTACGCAACTg  >  2:913562/1‑139 (MQ=255)                                                                                                                                       |                                                                                                                                      ACCTTTATAGAAAAACTGCCAGATATCTTCGATACGGTGCGCATCGCGACCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGTAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCTACGCAACTG  >  NC_000913/1654923‑1655190

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑