Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,656,744 G→A *187* (TAG→TAA speG → spermidine N(1)‑acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,656,7440GA100.0% 262.3 / NA 81*187* (TAG→TAAspeGspermidine N(1)‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (43/38);  total (43/38)

ATGCACGAGTTCTTTATTAATGGTCAATATCGTAATGCCATTCGCATGTGTATATTCCAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAGCATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATCAACAATGATTAGCTGACAGTCCACCGGATTAGCGTGACTGTCATATTCACAGCTCTGTTTTA  >  NC_000913/1656622‑1656874
                                                                                                                          |                                                                                                                                  
aTGCACGAGTTCTTTATTAATGGTCAATATCGTAATGCCATTCGCATGTGTATATTCCAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAatcga                                                                                                                    >  2:600730/1‑139 (MQ=255)
  gCACGAGTTCTTTATTAATGGTCAATATCGTAATGCCATTCGCATGTGTATATTCCAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGTCGACCGCACAATAACATTAGTAATAatcgatc                                                                                                                  >  2:1282227/1‑139 (MQ=255)
            tttATTAATGGTCAATATCGTAATGCCATTCGCATGTGTATATTCCAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGTCGACCGCACAATAACATTAGTAATAATCGATCGTATTTTTGa                                                                                                        <  1:1282227/139‑1 (MQ=255)
            tttATTAATGGTCAATATCGTAATGCCATTCGCATGTGTATATTCCAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGa                                                                                                        <  1:869975/139‑1 (MQ=255)
            tttATTAATGGTCAATATCGTAATGCCATTCGCATGTGTATATTCCAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGa                                                                                                        <  2:667605/139‑1 (MQ=255)
            tttATTAATGGTCAATATCGTAATGCCATTCGCATGTGTATATTCCAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGa                                                                                                        <  1:766351/139‑1 (MQ=255)
                   aTGGTCAATATCGTAATGCCATTCGCATGTGTATATTCCAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGa                                                                                                        >  1:258486/1‑132 (MQ=255)
                   aTGGTCAATATCGTAATGCCATTCGCATGTGTATATTCCAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGa                                                                                                        <  2:258486/132‑1 (MQ=255)
                      gTCAATATCGTAATGCCATTCGCATGTGTATATTCCAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAAc                                                                                              >  1:1237120/1‑139 (MQ=255)
                      gTCAATATCGTAATGCCATTCGCATGTGTATATTCCAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATGAATAATAATCGATCGTATTTTTGAAGGTGTAAAc                                                                                              >  2:941504/1‑139 (MQ=255)
                      gTCAATATCGTAATGCCATTCGCATGTGTATATTCCAGCATCAGAATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTTTAAAc                                                                                              >  1:1042551/1‑139 (MQ=255)
                        cAATATCGTAATGCCATTCGCATGTGTATATTCCAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGa                                                                                                        >  2:750202/1‑127 (MQ=255)
                        cAATATCGTAATGCCATTCGCATGTGTATATTCCAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGa                                                                                                        <  1:750202/127‑1 (MQ=255)
                              cGTAATGCCATTCGCATGTGTATATTCCAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGa                                                                                      >  1:347156/1‑139 (MQ=255)
                                 aaTGCCATTCGCATGTGTATATTCCAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGAcg                                                                                    <  2:609836/138‑1 (MQ=255)
                                 aaTGCCATTCGCATGTGTATATTCCAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGAcg                                                                                    >  1:609836/1‑138 (MQ=255)
                                   tGCCATTCGCATGTGTATATTCCAGCATCAGTATCTGGCAGAGCACAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGAcggcgg                                                                                >  2:1437550/1‑139 (MQ=255)
                                     ccATTCGCATGTGTATATTCCAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCggg                                                                               >  2:337274/1‑139 (MQ=255)
                                        ttCGCATGTGTATATTCCAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGttt                                                                            >  2:411706/1‑139 (MQ=255)
                                        ttCGCATGTGTATATTCCAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGttt                                                                            >  2:1285412/1‑139 (MQ=255)
                                          cGCATGTGTATATTCCAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGAcggc                                                                                  <  1:21361/131‑1 (MQ=255)
                                          cGCATGTGTATATTCCAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGAcggc                                                                                  >  2:21361/1‑131 (MQ=255)
                                            cATGTGTATATTCCAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACt                                                                        <  2:112484/139‑1 (MQ=255)
                                            cATGTGTATATTCCAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACt                                                                        <  1:337274/139‑1 (MQ=255)
                                            cATGTGTATATTCCAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACt                                                                        <  1:411706/139‑1 (MQ=255)
                                              tgtgTATATTCCAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTcc                                                                      <  2:347156/139‑1 (MQ=255)
                                                       tCCAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACt                                                                        >  2:91792/1‑128 (MQ=255)
                                                       tCCAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACt                                                                        <  1:91792/128‑1 (MQ=255)
                                                         cAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCGGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATcaacaa                                                           >  1:745060/1‑139 (MQ=255)
                                                           gCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATCAACAATg                                                         >  2:257008/1‑139 (MQ=255)
                                                            cATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACa                                                                                                                                  >  1:1114064/1‑65 (MQ=255)
                                                            cATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACa                                                                                                                                  <  2:1114064/65‑1 (MQ=255)
                                                            cATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTa                                                                          >  2:1452060/1‑121 (MQ=255)
                                                            cATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTa                                                                          <  1:1452060/121‑1 (MQ=255)
                                                            cATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATCAACAATGa                                                        >  1:1074442/1‑139 (MQ=255)
                                                            cATCAGTATCTGACAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATCAACAATGa                                                        >  2:1234081/1‑139 (MQ=255)
                                                              tCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACa                                                                                                                                  >  2:996632/1‑63 (MQ=255)
                                                              tCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACa                                                                                                                                  <  1:996632/63‑1 (MQ=255)
                                                                 gTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACt                                                                        <  1:506390/118‑1 (MQ=255)
                                                                 gTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACt                                                                        >  2:506390/1‑118 (MQ=255)
                                                                 gTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTtcatc                                                                >  2:80600/1‑126 (MQ=255)
                                                                 gTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTtcatc                                                                <  1:80600/126‑1 (MQ=255)
                                                                 gTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATCAACAATGATTAGc                                                   >  2:1415091/1‑139 (MQ=255)
                                                                    tCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACa                                                                                                                                  >  1:798973/1‑57 (MQ=255)
                                                                            agCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACa                                                                                                                                  >  2:409156/1‑49 (MQ=255)
                                                                            agCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACa                                                                                                                                  >  2:1469321/1‑49 (MQ=255)
                                                                            agCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACa                                                                                                                                  <  1:409156/49‑1 (MQ=255)
                                                                            agCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACa                                                                                                                                  <  1:1469321/49‑1 (MQ=255)
                                                                            agCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAatcgatc                                                                                                                  >  2:902316/1‑65 (MQ=255)
                                                                            agCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAatcgatc                                                                                                                  <  1:902316/65‑1 (MQ=255)
                                                                            agCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGa                                                                                                        >  1:1464509/1‑75 (MQ=255)
                                                                            agCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGa                                                                                                        <  2:1464509/75‑1 (MQ=255)
                                                                            agCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGAcgg                                                                                   >  2:1176294/1‑96 (MQ=255)
                                                                            agCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGAcgg                                                                                   <  1:1176294/96‑1 (MQ=255)
                                                                            agCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATCAACAATGATTAGCTGACAGTCCAc                                        >  2:1368022/1‑139 (MQ=255)
                                                                              cacaAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGa                                                                                                        >  2:1287940/1‑73 (MQ=255)
                                                                              cacaAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGa                                                                                                        <  1:1287940/73‑1 (MQ=255)
                                                                              cacaAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATCAACAATGATTAGCTGACAGTCCACCg                                      >  2:628542/1‑139 (MQ=255)
                                                                                       ccGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATCAACAATGATTAGCTGACAGTCCACCGGATTAGCGt                             >  1:38746/1‑139 (MQ=255)
                                                                                         gggTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTTTTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATCAACAATGATTAGCTGACAGTCCACCGGATTAGCGTGa                           <  1:1323117/139‑1 (MQ=255)
                                                                                         gggTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATCAACAATGATTAGCTGACAGTCCACCGGATTAGCGTGa                           <  1:941504/139‑1 (MQ=255)
                                                                                         gggTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATCAACAATGATTAGCTGACAGTCCACCGGATTAGCGTGa                           <  2:1074442/139‑1 (MQ=255)
                                                                                         gggTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATCAACAATGATTAGCTGACAGTCCACCGGATTAGCGTGa                           <  2:1237120/139‑1 (MQ=255)
                                                                                         gggTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTAATCAACAATGATTAGCTGACAGTCCACCGGATTAGCGTGa                           <  1:1368022/139‑1 (MQ=255)
                                                                                           gTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATCAACAATGATTAGCTGACAGTCCACCGGATTAGCGTGACt                         <  1:1415091/139‑1 (MQ=255)
                                                                                           gTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATCAACAATGATTAGCTGACAGTCCACCGGATTAGCGTGACt                         <  2:38746/139‑1 (MQ=255)
                                                                                                    tcatGAAGCCGGCCGCACAATAACATTAATAATAATCGATCGTATTTTTGATAGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATCAACAATGATTAGCTGACAGTCCACCGGATTAGCGTGACTGTCATATTc                >  1:852240/4‑139 (MQ=255)
                                                                                                     ccTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGa                                                                                                        <  2:340193/50‑1 (MQ=255)
                                                                                                     ccTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGa                                                                                                        >  1:340193/1‑50 (MQ=255)
                                                                                                         aaGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATCAACAATGATTAGCTGACAGTCCACCGGATTAGCGTGACTGTCATATTCACAGc           <  1:1285412/139‑1 (MQ=255)
                                                                                                          aGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGa                                                                                                        <  2:414392/45‑1 (MQ=255)
                                                                                                          aGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGa                                                                                                        >  1:414392/1‑45 (MQ=255)
                                                                                                          aGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATCAACAATGATTAGCTGACAGTCCACCGGATTAGCGTGACTGTCATATTCACAGct          <  1:1437550/139‑1 (MQ=255)
                                                                                                              gACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATcaa                                                              <  2:1328219/83‑1 (MQ=255)
                                                                                                              gACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATcaa                                                              >  1:1328219/1‑83 (MQ=255)
                                                                                                              gACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATCAACAATGATTAGCTGACAGTCCACCGGATTAGCGTGa                           >  2:325929/1‑118 (MQ=255)
                                                                                                              gACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATCAACAATGATTAGCTGACAGTCCACCGGATTAGCGTGa                           <  1:325929/118‑1 (MQ=255)
                                                                                                              gACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATCAACAATGATTAGCTGACAGTCCACCGGATTAGCGTGACTGTCATATTCACAGCTCTGt      >  1:531089/1‑139 (MQ=255)
                                                                                                              gACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATCAACAATGATTAGCTGACAGTCCACCGGATTAGCGTGACTGTCATATTCACAGCTCTGt      >  2:1381799/1‑139 (MQ=255)
                                                                                                              gACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATCAACAATGATTAGCTGACAGTCCACCGGATTAGCGTGACTGTCATATTCACAGCTCTGt      >  1:827967/1‑139 (MQ=255)
                                                                                                                  gCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATCAACAATGATTAGCTGACAGTCCACCGGATTAGCGTGACTGTCATATTCACAGCTCTGTTTTa  <  1:600730/139‑1 (MQ=255)
                                                                                                                          |                                                                                                                                  
ATGCACGAGTTCTTTATTAATGGTCAATATCGTAATGCCATTCGCATGTGTATATTCCAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAGCATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATCAACAATGATTAGCTGACAGTCCACCGGATTAGCGTGACTGTCATATTCACAGCTCTGTTTTA  >  NC_000913/1656622‑1656874

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: