Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,691,192	G→A	Q447Q (CAG→CAA)	ydgA →	DUF945 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,691,192	0	G	A	100.0%	197.7 / NA	61	Q447Q (CAG→CAA)	ydgA	DUF945 family protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/32);  total (29/32)

ATGCGAAACTGACCATTCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGGCAGAAAATGTCGTTAGAAGATTTTGTTGGCATGTTTGCGATGCCAGCTCTT  >  NC_000913/1691059‑1691324                                                                                                                                      |                                                                                                                                     aTGCGAAACTGACCATTCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGtt                                                                                                                                 <  1:350915/139‑1 (MQ=255)   gCGAAACTGACCATTCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTcc                                                                                                                               >  1:685839/1‑139 (MQ=255)   gCGAAACTGACCATTCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTcc                                                                                                                               >  2:1057354/1‑139 (MQ=255)            aCCATTCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGacca                                                                                                                      <  1:1057354/139‑1 (MQ=255)            aCCATTCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGacca                                                                                                                      <  1:747392/139‑1 (MQ=255)            aCCATTCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGacca                                                                                                                      >  1:985231/1‑139 (MQ=255)            aCCATTCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGacca                                                                                                                      <  1:149295/139‑1 (MQ=255)                 tCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAACAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTg                                                                                                                 <  1:306612/139‑1 (MQ=255)                  ccGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTGc                                                                                                                <  2:1397671/139‑1 (MQ=255)                   cGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTGCa                                                                                                               <  1:1349852/139‑1 (MQ=255)                           atGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGa                                                                                                                         <  1:789701/121‑1 (MQ=255)                           atGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGa                                                                                                                         >  2:789701/1‑121 (MQ=255)                           atGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTGCAGGACAATa                                                                                                       >  1:695138/1‑139 (MQ=255)                             ggCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTGCAGGACAATAcc                                                                                                     <  2:1139277/139‑1 (MQ=255)                                  cTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTGCAGGACAATACCATcac                                                                                                >  1:97303/1‑139 (MQ=255)                                    gAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTGCAGGACAATACCATcacca                                                                                              <  2:685839/139‑1 (MQ=255)                                    gAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTGCAGGACAATACCATcacca                                                                                              <  1:395194/139‑1 (MQ=255)                                        ttATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAAAAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAg                                                                                          <  1:1055590/139‑1 (MQ=255)                                         tATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGc                                                                                         <  2:854445/139‑1 (MQ=255)                                                 aGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTGCAg                                                                                                              >  1:396043/1‑110 (MQ=255)                                                 aGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTGCAg                                                                                                              <  2:396043/110‑1 (MQ=255)                                                       cGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTaa                                                                           >  1:1239412/1‑139 (MQ=255)                                                            cTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTc                                                                      >  2:367955/1‑139 (MQ=255)                                                            cTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTc                                                                      >  2:871280/1‑139 (MQ=255)                                                             tGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCa                                                                     >  1:102915/1‑139 (MQ=255)                                                               gAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTGCAgg                                                                                                             <  1:1473077/97‑1 (MQ=255)                                                               gAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTGCAgg                                                                                                             >  2:1473077/1‑97 (MQ=255)                                                                 aGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTGCAGGACAATACCATcaccac                                                                                             >  2:477952/1‑111 (MQ=255)                                                                 aGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTGCAGGACAATACCATcaccac                                                                                             <  1:477952/111‑1 (MQ=255)                                                                       tCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTa                                                           >  1:467673/1‑139 (MQ=255)                                                                         aGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGa                                                                                                                         <  1:688753/75‑1 (MQ=255)                                                                         aGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGa                                                                                                                         >  2:688753/1‑75 (MQ=255)                                                                              gATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGc                                                                                     >  2:609442/1‑106 (MQ=255)                                                                              gATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGc                                                                                     <  1:609442/106‑1 (MQ=255)                                                                              gATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACg                                                                         >  1:820924/1‑118 (MQ=255)                                                                              gATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACg                                                                         <  2:820924/118‑1 (MQ=255)                                                                                   aGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGacca                                                                                                                      <  1:1037017/68‑1 (MQ=255)                                                                                   aGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGacca                                                                                                                      >  1:1133226/1‑68 (MQ=255)                                                                                   aGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGacca                                                                                                                      <  2:1133226/68‑1 (MQ=255)                                                                                   aGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGacca                                                                                                                      >  2:1037017/1‑68 (MQ=255)                                                                                   aGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGGCAGaaa                                               >  2:121601/1‑139 (MQ=255)                                                                                   aGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGGCAGaaa                                               <  1:1294421/139‑1 (MQ=255)                                                                                     cGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGacca                                                                                                                      <  2:126203/66‑1 (MQ=255)                                                                                     cGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGacca                                                                                                                      >  1:126203/1‑66 (MQ=255)                                                                                      gAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGGCAGAAAATg                                            <  1:871280/139‑1 (MQ=255)                                                                                         aaaaCTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGGCAGAAAATGTCg                                         >  1:1352795/1‑139 (MQ=255)                                                                                             cTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGGCAGAAAATGTCGTTag                                     <  2:985231/139‑1 (MQ=255)                                                                                              tGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGGCAGAAAATGTCGTTaga                                    <  2:467673/139‑1 (MQ=255)                                                                                                                   gTGCATCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGGCAGAAAATGTCGTTAGAAGATTTTGTTGGCATGTTTGc               <  2:1239412/139‑1 (MQ=255)                                                                                                                       aTCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTGCAGGACAATACCATcacca                                                                                              >  1:642165/1‑56 (MQ=255)                                                                                                                       aTCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTGCAGGACAATACCATcacca                                                                                              <  2:642165/56‑1 (MQ=255)                                                                                                                       aTCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGGCAGAAAATGTCGTTAGAAGATTTTGTTGGCATGTTTGCGATg           >  2:548198/1‑139 (MQ=255)                                                                                                                           gCAATGGGGCAAATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGGCAGAAAATGTCGTTAGAAGATTTTGTTGGCATGTTTGCGATGCCAg       >  2:1397809/1‑139 (MQ=255)                                                                                                                              aTGGGGCAAATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTg                                                                                      >  1:639541/1‑57 (MQ=255)                                                                                                                              aTGGGGCAAATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTg                                                                                      <  2:639541/57‑1 (MQ=255)                                                                                                                               tGGGGCAAATGTTCCGTCTGCCCACCTTGCAGGACAATACCATCACCACCAGCCTGc                                                                                     <  1:1466392/57‑1 (MQ=255)                                                                                                                               tGGGGCAAATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGc                                                                                     >  2:1466392/1‑57 (MQ=255)                                                                                                                                ggggCAAATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGGCAGAAAATGTCGTTAGAAGATTTTGTTGGCATGTTTGCGATGCCAGCTCtt  <  2:695138/139‑1 (MQ=255)                                                                                                                                ggggCAAATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGGCAGAAAATGTCGTTAGAAGATTTTGTTGGCATGTTTGCGATGCCAGCTCtt  >  2:385038/1‑139 (MQ=255)                                                                                                                                 gggCAAATGTTCCGTCTGACCACCTTGCAGGACAATACCAt                                                                                                   <  1:1378919/41‑1 (MQ=255)                                                                                                                                 gggCAAATGTTCCGTCTGACCACCTTGCAGGACAATACCAt                                                                                                   >  2:1378919/1‑41 (MQ=255)                                                                                                                                      |                                                                                                                                     ATGCGAAACTGACCATTCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGGCAGAAAATGTCGTTAGAAGATTTTGTTGGCATGTTTGCGATGCCAGCTCTT  >  NC_000913/1691059‑1691324

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑