Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	167,743	C→T	T87I (ACC→ATC)	fhuA →	ferrichrome outer membrane transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	167,743	0	C	T	100.0%	175.4 / NA	54	T87I (ACC→ATC)	fhuA	ferrichrome outer membrane transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/31);  total (23/31)

CACCGTTACCGCTGCACCTGCGCCGCAAGAAAGCGCATGGGGGCCTGCTGCAACTATTGCGGCGCGACAGTCTGCTACCGGCACTAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGACCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCAACACCTATGACCACCTGATCATTCGCGGCTTTGCGGCAGAAG  >  NC_000913/167609‑167877                                                                                                                                       |                                                                                                                                       cACCGTTACCGCTGCACCTGCGCCGCAAGAAAGCGCATGGGGGCCTGCTGCAACTATTGCGGCGCGACAGTCTGCTACCGGCACTAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGAtcgcc                                                                                                                                    >  1:522444/1‑139 (MQ=255)      ttACCGCTGCACCTGCGCCGCAAGAAAGCGCATGGGGGCCTGCTGCAACTATTGCGGCGCGACAGTCTGCTACCGGCACTAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAaga                                                                                                                               <  2:3349/139‑1 (MQ=255)        aCCGCTGCACCTGCGCCGCAAGAAAGCGCATGGGGGCCTGCTGCAACTATTGCGGCGCGACAGTCTGCTACCGGCACTAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAgaga                                                                                                                             >  2:1311939/1‑139 (MQ=255)             tGCACCTGCGCCGCAAGAAAGCGCATGGGGGCCTGCTGCAACTATTGCGGCGCGACAGTCTGCTACCGGCACTAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGgcg                                                                                                                        <  1:599898/139‑1 (MQ=255)             tGCACCTGCGCCGCAAGAAAGCGCATGGGGGCCTGCTGCAACTATTGCGGCGCGACAGTCTGCTACCGGCACTAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGgcg                                                                                                                        <  2:1307004/139‑1 (MQ=255)              gCACCTGCGCCGCAAGAAAGCGCATGGGGGCCTGCTGCAACTATTGCGGCGCGACAGTCTGCTACCGGCACTAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGgcgc                                                                                                                       >  2:229477/1‑139 (MQ=255)                 ccTGCGCCGCAAGAAAGCGCATGGGGGCCTGCTGCAACTATTGCGGCGCGACAGTCTGCTACCGGCACTAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGc                                                                                                                    >  2:710115/1‑139 (MQ=255)                          cAAGAAAGCGCATGGGGGCCTGCTGCAACTATTGCGGCGCGACAGTCTGCTACCGGCACTAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGa                                                                                                           >  2:95349/1‑139 (MQ=255)                            aGAAAGCGCATGGGGGCCTGCTGCAACTATTGCGGCGCGACAGTCTGCTACCGGCACTAAATCCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAg                                                                                                         <  1:708564/139‑1 (MQ=255)                            aGAAAGCGCATGGGGGCCTGCTGCAACTATTGCGGCGCGACAGTCTGCTACCGGCACTAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAg                                                                                                         <  1:423362/139‑1 (MQ=255)                                  cgcATGGGGGCCTGCTGCAACTATTGCGGCGCGACAGTCTGCTACCGGCACTAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGaa                                                                                                          <  1:104596/132‑1 (MQ=255)                                  cgcATGGGGGCCTGCTGCAACTATTGCGGCGCGACAGTCTGCTACCGGCACTAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGaa                                                                                                          >  2:104596/1‑132 (MQ=255)                                  cgcATGGGGGCCTGCTGCAACTATTGCGGCGCGACAGTCTGCTACCGGCACTAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTa                                                                                                   >  2:691831/1‑139 (MQ=255)                                      tGGGGGCCTGCTGCAACTATTGCGGCGCGACAGTCTGCTACCGGCACTAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAaag                                                                                               <  2:522444/139‑1 (MQ=255)                                       gggggCCTGCTGCAACTATTGCGGCGCGACAGTCTGCTACCGGCACTAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAaaga                                                                                              <  2:855285/139‑1 (MQ=255)                                       gggggCCTGCTGCAACTATTGCGGCGCGACAGTCTGCTACCGGCACTAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAaaga                                                                                              <  1:1186418/139‑1 (MQ=255)                                        ggggCCTGCTGCAACTATTGCGGCGCGACAGTCTGCTACCGGCACTAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAaagaa                                                                                             <  1:725823/139‑1 (MQ=255)                                        ggggCCTGCTGCAACTATTGCGGCGCGACAGTCTGCTACCGGCACTAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAaagaa                                                                                             >  2:1154036/1‑139 (MQ=255)                                           gCCTGCTGCAACTATTGCGGCGCGACAGTCTGCTACCGGCACTAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAgcg                                                                                          <  1:691831/139‑1 (MQ=255)                                                 tgcAACTATTGCGGCGCGACAGTCTGCTACCGGCACTAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGaa                                                                                                          >  2:235892/1‑117 (MQ=255)                                                 tgcAACTATTGCGGCGCGACAGTCTGCTACCGGCACTAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGaa                                                                                                          <  1:235892/117‑1 (MQ=255)                                                   cAACTATTGCGGCGCGACAGTCTGCTACCGGCACTAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTa                                                                                  <  1:1439868/139‑1 (MQ=255)                                                               cgcgACAGTCTGCTACCGGCACTAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGgtgt                                                                      <  1:752508/139‑1 (MQ=255)                                                               cgcgACAGTCTGCTACCGGCACTAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGgtgt                                                                      <  2:160107/139‑1 (MQ=255)                                                                            tACCGGCACTAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTAcg                                                         >  2:748806/1‑139 (MQ=255)                                                                             aCCGGCACTAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTAcgc                                                        <  2:111921/139‑1 (MQ=255)                                                                              ccGGCACTAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTAcgcg                                                       <  1:710115/139‑1 (MQ=255)                                                                                ggCACTAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTg                                                     <  1:748806/139‑1 (MQ=255)                                                                                 gCACTAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGcc                                                                            >  1:671862/1‑115 (MQ=255)                                                                                 gCACTAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGcc                                                                            <  2:671862/115‑1 (MQ=255)                                                                                 gCACTAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGgt                                                                        >  2:315095/1‑119 (MQ=255)                                                                                 gCACTAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGgt                                                                        <  1:315095/119‑1 (MQ=255)                                                                                  cACTAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGtct                                                                    >  2:860017/1‑122 (MQ=255)                                                                                  cACTAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGtct                                                                    <  1:860017/122‑1 (MQ=255)                                                                                  cACTAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGgc                                                   <  1:95349/139‑1 (MQ=255)                                                                                   aCTAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGgcg                                                  <  1:1294989/139‑1 (MQ=255)                                                                                     tAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCa                                                >  1:1142100/1‑139 (MQ=255)                                                                                     tAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCa                                                <  1:557643/139‑1 (MQ=255)                                                                                       aaaCCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATc                                              >  1:519491/1‑139 (MQ=255)                                                                                        aaCCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATcc                                             <  1:1154036/139‑1 (MQ=255)                                                                                          ccGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCaa                                           <  2:148635/139‑1 (MQ=255)                                                                                             aTACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGgtgt                                                                      >  1:948984/1‑109 (MQ=255)                                                                                             aTACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGgtgt                                                                      <  2:948984/109‑1 (MQ=255)                                                                                                                 ccACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCt                                                                                        <  1:679383/71‑1 (MQ=255)                                                                                                                 ccACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCt                                                                                        >  2:679383/1‑71 (MQ=255)                                                                                                                       tCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCAACACCTATGACCACCTGATCATTCGCGGCt              >  1:419648/1‑139 (MQ=255)                                                                                                                               tgttgtGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCAACACCTATGACCACCTGATCATTCGCGGCTTTGCGGCa      >  1:97158/1‑139 (MQ=255)                                                                                                                                 ttgtGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCAACACCTATGACCACCTGATCATTCGCgg                <  1:216888/127‑1 (MQ=255)                                                                                                                                 ttgtGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCAACACCTATGACCACCTGATCATTCGCgg                >  2:216888/1‑127 (MQ=255)                                                                                                                                 ttgtGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCAACACCTATGACCACCTGATCATTCGCGGCTTTGCGGCAGa    >  1:1423176/1‑139 (MQ=255)                                                                                                                                   gtgATCGCCGAAGAGATGGCGCTGCATCAGCCGAAg                                                                                                         >  1:1397003/1‑36 (MQ=255)                                                                                                                                   gtgATCGCCGAAGAGATGGCGCTGCATCAGCCGAAg                                                                                                         <  2:1397003/36‑1 (MQ=255)                                                                                                                                   gtgATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCtt                                                                                       >  1:136059/1‑54 (MQ=255)                                                                                                                                   gtgATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCtt                                                                                       <  2:136059/54‑1 (MQ=255)                                                                                                                                   gtgATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCAACACCTATGACCACCTGATCATTCGCGGCTTTGCGGCAGAAg  >  1:292102/1‑139 (MQ=255)                                                                                                                                       |                                                                                                                                       CACCGTTACCGCTGCACCTGCGCCGCAAGAAAGCGCATGGGGGCCTGCTGCAACTATTGCGGCGCGACAGTCTGCTACCGGCACTAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGACCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCAACACCTATGACCACCTGATCATTCGCGGCTTTGCGGCAGAAG  >  NC_000913/167609‑167877

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑