Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,788,278 G→A *278* (TAG→TAA)  ppsR → PEP synthase kinase and PEP synthase pyrophosphorylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,788,2780GA100.0% 150.0 / NA 47*278* (TAG→TAA) ppsRPEP synthase kinase and PEP synthase pyrophosphorylase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/24);  total (23/24)

GATGGAAGTCGCGGAAGTGGAAGCCTTGTACCGTAAAAATCAGATCCCGTGGATTAACAGTACCAATTATTCGGTAGAAGAGATTGCCACCAAGATCCTCGATATCATGGGCCTTAGTCGCCGAATGTACTAGAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCGGCGAGGTGTGACACACCTCGCACTTGAAATCAGCAGCGATTGGTTTATCGTGATGCGCATCACTTCCCGGCAGT  >  NC_000913/1788146‑1788399
                                                                                                                                    |                                                                                                                         
gATGGAAGTCGCGGAAGTGGAAGCCTTGTACCGTAAAAATCAGATCCCGTGGATTAACAGTACCAATTATTCGGTAGAAGAGATTGCCACCAAGATCCTCGATATCATGGGCCTTAGTCGCCGAATGTACTAAAGAACt                                                                                                                     >  2:1419025/1‑139 (MQ=255)
              aaGTGGAAGCCTTGTACCGTAAAAATCAGATCCCGTGGATTAACAGTACCAATTATTCGGTAGAAGAGATTGCCACCAAGATCCTCGATATCATGGGCCTTAGTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTa                                                                                                       <  2:1328824/139‑1 (MQ=255)
                      gCCTTGTACCGTAAAAATCAGATCCCGTGGATTAACAGTACCAATTATTCGGTAGAAGAGATTGCCACCAAGATCCTCGATATCATGGGCCTTAGTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTg                                                                                               <  1:580550/139‑1 (MQ=255)
                                    aaaTCAGATCCCGTGGATTAACAGTACCAATTATTCGGTAGAAGAGATTGCCACCAAGATCCTCGATATCATGGGCCTTAGTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAacc                                                                                 <  1:858681/139‑1 (MQ=255)
                                                gTGGATTAACAGTACCAATTATTCGGTAGAAGAGATTGCCACCAAGATCCTCGATATCATGGGCCTTAGTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCGGCGAGgt                                                                     <  2:1009388/139‑1 (MQ=255)
                                                 tGGATTAACAGTACCAATTATTCGGTAGAAGAGATTGCCACCAAGATCCTCGATATCATGGGCCTTAGTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCGGCGAGgtg                                                                    >  2:1473184/1‑139 (MQ=255)
                                                        aCAGTACCAATTATTCGGTAGAAGAGATTGCCACCAAGATCCTCGATATCATGGGCCTTAGTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCGGCGAGGTGTGacaca                                                             <  1:1419025/139‑1 (MQ=255)
                                                             aCCAATTATTAGGTAGAAGAGATTGCAACCAAGATCCTAGATATCATGGGCCTTAGTCGCCGAATGTACTAAAGAAATAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCAACCGACGAGGTGTGACACACCTCg                                                        >  1:984221/1‑139 (MQ=255)
                                                                 attattCGGTAGAAGAGATTGCCACCAAGATCCTCGATATCATGGGCCTTAGTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGTTa                                                                                            >  1:1319500/1‑99 (MQ=255)
                                                                 attattCGGTAGAAGAGATTGCCACCAAGATCCTCGATATCATGGGCCTTAGTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGTTa                                                                                            <  2:1319500/99‑1 (MQ=255)
                                                                      tCGGTAGAAGAGATTGCCACCAAGATGCTCGATATCATGGGCCTTAGTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCGGCGAGGTGTGACACACCTCGCACTTGaaa                                               >  2:1331273/1‑139 (MQ=255)
                                                                               gagaTTGCCACCAAGATCCTCGATATCATGGGCCTTAGTCGCCGAATGTACTaaag                                                                                                                         >  2:578259/1‑56 (MQ=255)
                                                                               gagaTTGCCACCAAGATCCTCGATATCATGGGCCTTAGTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGTTa                                                                                            <  1:373553/85‑1 (MQ=255)
                                                                               gagaTTGCCACCAAGATCCTCGATATCATGGGCCTTAGTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGTTa                                                                                            >  2:373553/1‑85 (MQ=255)
                                                                               gagaTTGCCACCAAGATCCTCGATATCATGGGCCTTAGTCGCCGAATGTAATAAAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCGGCGAGGTGTGACACACCTCGCCCTTGAAATCAGCAGCg                                      >  2:419739/1‑139 (MQ=255)
                                                                                  aTTGCCACCAAGATCCTCGATATCATGGGCCTTAGTCGCCGAATGTACTAAAGAACTAGTGCATTAGc                                                                                                          <  2:224637/68‑1 (MQ=255)
                                                                                  aTTGCCACCAAGATCCTCGATATCATGGGCCTTAGTCGCCGAATGTACTAAAGAACTAGTGCATTAGc                                                                                                          >  1:224637/1‑68 (MQ=255)
                                                                                    tGCCACCAAGATCCTCGATATCATGGGCCTTAGTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCGGCGAGGTGTGACACACCTCGCACTTGaa                                                >  1:1257164/1‑124 (MQ=255)
                                                                                    tGCCACCAAGATCCTCGATATCATGGGCCTTAGTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCGGCGAGGTGTGACACACCTCGCACTTGaa                                                <  2:1257164/124‑1 (MQ=255)
                                                                                    tGCCACCAAGATCCTCGATATCATGGGCCTTAGTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCGGCGAGGTGTGACACACCTCGCACTTGAAATCAGCAGCGATTgg                                 >  1:537777/1‑139 (MQ=255)
                                                                                          caAGATCCTCGATATCATGGGCCTTAGTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCGGCg                                                                         >  1:400621/1‑93 (MQ=255)
                                                                                          caAGATCCTCGATATCATGGGCCTTAGTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCGGCg                                                                         <  2:400621/93‑1 (MQ=255)
                                                                                             gATCCTCGATATCATGGGCCTTAGTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCGGCGAGGTGTGACACACCTCGCACTTGAAATCAGCAGCGATTGGTTTATCGTg                        >  1:1021333/1‑139 (MQ=255)
                                                                                             gATCCTCGATATCATGGGCCTTAGTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCGGCGAGGTGTGACACACCTCGCACTTGAAATCAGCAGCGATTGGTTTATCGTg                        <  1:654096/139‑1 (MQ=255)
                                                                                                ccTCGATATCATGGGCCTTAGTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCGGCGAGGTGTGACACACCTCGCACTTGAAATCAGCAGCGATTGGTTTATCGTGATg                     <  2:537777/139‑1 (MQ=255)
                                                                                                ccTCGATATCATGGGCCTTAGTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCGGCGAGGTGTGACACACCTCGCACTTGAAATCAGCAGCGATTGGTTTATCGTGATg                     <  1:1331273/139‑1 (MQ=255)
                                                                                                     atatCATGGGCCTTAGTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCGGCGAGGTGTGACACACCTCGCACTTGAAATCAGCAGCGATTGGTTTATCGTGATGCGCAt                <  1:419739/139‑1 (MQ=255)
                                                                                                       atCATGGGCCTTAGTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCGGCGAGGTGTGACACACCTCGCACTTGAAATCAGCAGCGATTGGTTTATCGTGATGCGCATCa              <  1:414322/139‑1 (MQ=255)
                                                                                                           tGGGCCTTAGTCGCCGAATGTACTAAAGAACTAGTGCATTAGc                                                                                                          >  1:2039/1‑43 (MQ=255)
                                                                                                           tGGGCCTTAGTCGCCGAATGTACTAAAGAACTAGTGCATTAGc                                                                                                          <  2:2039/43‑1 (MQ=255)
                                                                                                           tGGGCCTTAGTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCGGCGAGGTGTGACACACCTCGCACTTGAAATCAGCAGCGa                                     <  1:1470587/112‑1 (MQ=255)
                                                                                                           tGGGCCTTAGTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCGGCGAGGTGTGACACACCTCGCACTTGAAATCAGCAGCGa                                     >  2:1470587/1‑112 (MQ=255)
                                                                                                              gCCTTAGTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTa                                                                                    <  1:49269/62‑1 (MQ=255)
                                                                                                              gCCTTAGTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTa                                                                                    >  2:49269/1‑62 (MQ=255)
                                                                                                               ccTTAGTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCGGCGAGGTGTGACACACCTCGCACTTGAAATCAGCAGCGATTGGTTTATCGTGATGCGCATCACTTCCCgg      <  2:1021333/139‑1 (MQ=255)
                                                                                                                cTTAGTCGCCGAATGTACTAAAGAACTAGTGCAtt                                                                                                             >  1:57077/1‑35 (MQ=255)
                                                                                                                cTTAGTCGCCGAATGTACTAAAGAACTAGTGCAtt                                                                                                             <  2:57077/35‑1 (MQ=255)
                                                                                                                cTTAGTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCGGCGAGGTGTGACACACCTCGCACTTGAAATCAGCAGCGa                                     <  2:1415670/107‑1 (MQ=255)
                                                                                                                cTTAGTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCGGCGAGGTGTGACACACCTCGCACTTGAAATCAGCAGCGa                                     >  1:1415670/1‑107 (MQ=255)
                                                                                                                  tAGTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCGGCGAGGTGTGACACACCTCGCACTTGAAATCAGCAGCGATTGGTTTATCGTGATGCGCATCACTTCCCGGCAg   >  2:437878/1‑139 (MQ=255)
                                                                                                                   aGTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCgg                                                                           <  2:1355571/66‑1 (MQ=255)
                                                                                                                   aGTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCgg                                                                           >  1:1355571/1‑66 (MQ=255)
                                                                                                                   aGTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCGGCGAGGTGTGACACACCTCGCACTTGAAATCAGCAGCGATTGGTTTATCGTGATGCGCATCACTTCCCGGCAGt  >  1:1223517/1‑139 (MQ=255)
                                                                                                                   aGTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCGGCGAGGTGTGACACACCTCGCACTTGAAATCAGCAGCGATTGGTTTATCGTGATGCGCATCACTTCCCGGCAGt  >  1:480935/1‑139 (MQ=255)
                                                                                                                    gTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCGGCGAGGTGTGACACACCTCGCACTTGAAATcagca                                         >  2:249204/1‑99 (MQ=255)
                                                                                                                    gTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCGGCGAGGTGTGACACACCTCGCACTTGAAATcagca                                         <  1:249204/99‑1 (MQ=255)
                                                                                                                         cGAATGTACTAAAGAACTAGTGCATTAGCTTAtttttt                                                                                                 >  1:1153460/1‑38 (MQ=255)
                                                                                                                         cGAATGTACTAAAGAACTAGTGCATTAGCTTAtttttt                                                                                                 <  2:1153460/38‑1 (MQ=255)
                                                                                                                                    |                                                                                                                         
GATGGAAGTCGCGGAAGTGGAAGCCTTGTACCGTAAAAATCAGATCCCGTGGATTAACAGTACCAATTATTCGGTAGAAGAGATTGCCACCAAGATCCTCGATATCATGGGCCTTAGTCGCCGAATGTACTAGAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCGGCGAGGTGTGACACACCTCGCACTTGAAATCAGCAGCGATTGGTTTATCGTGATGCGCATCACTTCCCGGCAGT  >  NC_000913/1788146‑1788399

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑