Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,874,078	C→T	*35* (TAG→TAA)	yoaI ←	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,874,078	0	C	T	100.0%	195.9 / NA	61	*35* (TAG→TAA)	yoaI	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/33);  total (28/33)

CTCTTCGAACGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAACTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAGCTGTTTTCA  >  NC_000913/1873952‑1874211                                                                                                                               |                                                                                                                                      ctctTCGAACGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGt                                                                                                                           <  1:1461471/139‑1 (MQ=255)      cGAACGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGAt                                                                                                                      >  2:795735/1‑139 (MQ=255)         aCGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATaag                                                                                                                   >  2:872715/1‑139 (MQ=255)             tttGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAagaaga                                                                                                               <  2:93926/139‑1 (MQ=255)                   gaAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGAc                                                                                                         <  1:8892/139‑1 (MQ=255)                       tCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACaaaa                                                                                                     >  1:1097472/1‑139 (MQ=255)                       tCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACaaaa                                                                                                     >  2:761413/1‑139 (MQ=255)                        cGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTATCCGGTCCGTTCGATAAGAAGAACGGACAAAAc                                                                                                    <  1:872715/139‑1 (MQ=255)                        cGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAAc                                                                                                    <  2:543300/139‑1 (MQ=255)                              cAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTac                                                                                              >  1:560876/1‑139 (MQ=255)                                        aTGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAAAAGCAATg                                                                                    >  2:180430/1‑139 (MQ=255)                                           cTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACaaa                                                                                                      >  1:734820/1‑118 (MQ=255)                                           cTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACaaa                                                                                                      <  2:734820/118‑1 (MQ=255)                                           cTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCa                                                                                 >  2:826846/1‑139 (MQ=255)                                              gATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCg                                                                                                                                 >  2:625962/1‑88 (MQ=255)                                              gATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCg                                                                                                                                 <  1:625962/88‑1 (MQ=255)                                                tAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCaaaaaa                                                                            >  2:87635/1‑139 (MQ=255)                                                     ccGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATg                                                                       <  1:87635/139‑1 (MQ=255)                                                     ccGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATg                                                                       <  1:795735/139‑1 (MQ=255)                                                       gTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGAc                                                                     <  2:1097472/139‑1 (MQ=255)                                                        ttATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTA‑CCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGt                                                                   >  1:617845/1‑139 (MQ=255)                                                         tATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGt                                                                   <  2:560876/139‑1 (MQ=255)                                                                     cgcgGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAgtgt                                                       >  1:941919/1‑139 (MQ=255)                                                                     cgcgGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAgtgt                                                       >  2:1230719/1‑139 (MQ=255)                                                                           ttattTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGAc                                                 <  2:670331/139‑1 (MQ=255)                                                                           ttattTGCGCATTGCGCAACCAGAACTGGTCAATCTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGAc                                                 <  2:941919/139‑1 (MQ=255)                                                                                    cattgcgcaACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTcc                                                                                                                             <  1:748180/54‑1 (MQ=255)                                                                                    cattgcgcaACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTcc                                                                                                                             >  2:748180/1‑54 (MQ=255)                                                                                     attgcgcaACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGa                                       <  1:1230719/139‑1 (MQ=255)                                                                                      ttgcgcaACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAg                                                                                                                                    >  1:1266876/1‑45 (MQ=255)                                                                                      ttgcgcaACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAg                                                                                                                                    <  2:1266876/45‑1 (MQ=255)                                                                                       tgcgcaACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATc                                     <  1:180430/139‑1 (MQ=255)                                                                                        gcgcaACCAGAACTGTTCAATTTCCGCCGCACTGCGTAATTAGCCGGt                                                                                                                               <  1:381521/48‑1 (MQ=39)                                                                                        gcgcaACCAGAACTGTTCAATTTCCGCCGCACTGCGTAATTAGCCGGt                                                                                                                               >  2:381521/1‑48 (MQ=255)                                                                                         cgcaACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTAATCGt                                   >  1:600529/1‑139 (MQ=255)                                                                                                 gAACTGGTCAATTTCCGCCGCACTGCGTAATTTGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCa                           >  2:623756/1‑139 (MQ=255)                                                                                                 gAACTGGTCAATTTCCGCCGCACTGCGTAATTA‑CCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCAt                          <  2:617845/139‑1 (MQ=255)                                                                                                  aaCTGGTCAATTTCCGCCGCACTGCGTAATTTGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCAt                          <  1:623756/139‑1 (MQ=255)                                                                                                  aaCTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCAt                          <  1:826846/139‑1 (MQ=255)                                                                                                      ggTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATg                                                                       >  1:588551/1‑90 (MQ=255)                                                                                                      ggTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATg                                                                       <  2:588551/90‑1 (MQ=255)                                                                                                       gTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGcccc                     >  1:1322450/1‑139 (MQ=255)                                                                                                       gTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGcccc                     <  2:540779/139‑1 (MQ=255)                                                                                                        tCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGt                                                                                                                           <  2:336506/36‑1 (MQ=255)                                                                                                        tCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGt                                                                                                                           >  1:336506/1‑36 (MQ=255)                                                                                                        tCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAgaagaa                                                                                                              >  1:994647/1‑49 (MQ=255)                                                                                                        tCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAgaagaa                                                                                                              <  2:994647/49‑1 (MQ=255)                                                                                                        tCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGa                                                                                                          <  1:1368795/53‑1 (MQ=255)                                                                                                        tCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGa                                                                                                          >  2:1368795/1‑53 (MQ=255)                                                                                                        tCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTg                                                                  <  2:1048607/93‑1 (MQ=255)                                                                                                        tCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTg                                                                  >  1:1048607/1‑93 (MQ=255)                                                                                                                 gccgcACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGa                                       >  2:30762/1‑111 (MQ=255)                                                                                                                 gccgcACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGa                                       <  1:30762/111‑1 (MQ=255)                                                                                                                 gccgcACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAg           <  2:865485/139‑1 (MQ=255)                                                                                                                     cACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGAc                                                                                                         >  2:434371/1‑41 (MQ=255)                                                                                                                     cACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGAc                                                                                                         <  1:434371/41‑1 (MQ=255)                                                                                                                       cTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAGCTGttt     <  2:1322450/139‑1 (MQ=255)                                                                                                                         gCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAGCTGTTTTc   <  1:1148065/139‑1 (MQ=255)                                                                                                                          cGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAAcc                                                                                                   >  2:148202/1‑42 (MQ=255)                                                                                                                          cGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAAcc                                                                                                   <  1:148202/42‑1 (MQ=255)                                                                                                                          cGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAGCTGTTTTCa  >  1:1019648/1‑139 (MQ=255)                                                                                                                               |                                                                                                                                      CTCTTCGAACGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAACTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAGCTGTTTTCA  >  NC_000913/1873952‑1874211

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑