Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,084,773 G→A G251G (GGC→GGT)  yeeE ← UPF0394 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,084,7730GA99.6% 726.8 / NA 224G251G (GGC→GGT) yeeEUPF0394 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (110/113);  total (110/114)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 226 total aligned reads displayed.

TGGCACCGAAGCCCATCAGTACACCACCTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACGCCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAGCGCAATCAAACCGAT  >  NC_000913/2084637‑2084907
                                                                                                                                        |                                                                                                                                      
tGGCACCGAAGCCCATCAGTACACCACCTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACAcc                                                                                                                                      <  2:1145530/139‑1 (MQ=255)
 ggCACCGAAGCCCATCAGTACACCACCTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACAccc                                                                                                                                     <  1:652852/139‑1 (MQ=255)
 ggCACCGAAGCCCATCAGTACACCACCTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACAccc                                                                                                                                     <  1:919458/139‑1 (MQ=255)
  gCACCGAAGCCCATCAGTACACCACCTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACAcccc                                                                                                                                    <  2:553035/139‑1 (MQ=255)
  gCACCGAAGCCCATCAGTACACCACCTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACAcccc                                                                                                                                    >  2:142196/1‑139 (MQ=255)
    aCCGAAGCCCATCAGTACACCACCTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGTGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGA‑CCCTAACACCAGGAAAACACCCCAGt                                                                                                                                 <  2:1188068/139‑1 (MQ=255)
    aCCGAAGCCCATCAGTACACCACCTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAg                                                                                                                                  >  2:854492/1‑139 (MQ=255)
        aaGCCCATCAGTACACCACCTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACAcc                                                                                                                                      <  2:300885/131‑1 (MQ=255)
        aaGCCCATCAGTACACCACCTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACAcc                                                                                                                                      >  1:300885/1‑131 (MQ=255)
        aaGCCCATCAGTACACCACCTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGt                                                                                                                                 >  2:966821/1‑136 (MQ=255)
        aaGCCCATCAGTACACCACCTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTaa                                                                                                                              <  1:1003039/139‑1 (MQ=255)
         aGCCCATCAGTACACCACCTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAAt                                                                                                                             >  1:195666/1‑139 (MQ=255)
                          ccTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCGCGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCcg                                                                                                            >  1:61256/1‑139 (MQ=255)
                          ccTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGCCCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCcg                                                                                                            <  1:210607/139‑1 (MQ=255)
                          ccTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACAcc                                                                                                                                      <  2:1358292/113‑1 (MQ=255)
                          ccTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACAcc                                                                                                                                      >  1:69011/1‑113 (MQ=255)
                          ccTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACAcc                                                                                                                                      >  1:1358292/1‑113 (MQ=255)
                          ccTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCcg                                                                                                            >  2:1443177/1‑139 (MQ=255)
                          ccTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCcg                                                                                                            >  1:1212215/1‑139 (MQ=255)
                           cTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACAcc                                                                                                                                      <  2:807483/112‑1 (MQ=255)
                             aaCCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTAt                                                                                                                         <  2:156607/123‑1 (MQ=255)
                             aaCCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGa                                                                                                         >  1:845380/1‑139 (MQ=255)
                               cccGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGt                                                                                                                                 >  2:1106598/1‑113 (MQ=255)
                                     aCGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAAt                                                                                                 >  2:767427/1‑139 (MQ=255)
                                       gTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTg                                                                                               <  1:1262297/139‑1 (MQ=255)
                                        tAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGc                                                                                              <  2:195666/139‑1 (MQ=255)
                                         aaTGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAGTAAATGACCCCACGAAGATCCCTAACCCCAGGAAAACGCCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCa                                                                                             <  1:200707/139‑1 (MQ=255)
                                         aaTGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCa                                                                                             <  1:784132/139‑1 (MQ=255)
                                         aaTGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCa                                                                                             <  1:451833/139‑1 (MQ=255)
                                          aTGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAg                                                                                            <  1:767427/139‑1 (MQ=255)
                                           tgttgtTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGa                                                                                           <  2:499384/139‑1 (MQ=255)
                                                tttgtGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGt                                                                                                                                 >  1:469260/1‑96 (MQ=255)
                                                tttgtGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGa                                                                                                    <  2:963101/125‑1 (MQ=255)
                                                tttgtGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATAtt                                                                                      >  2:939801/1‑139 (MQ=255)
                                                  tgtgCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATg                                                                                                                            >  1:243725/1‑99 (MQ=255)
                                                   gtgCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTAt                                                                                                                         >  1:858568/1‑101 (MQ=255)
                                                   gtgCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGc                                                                                   <  2:766182/139‑1 (MQ=255)
                                                   gtgCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGc                                                                                   <  1:803969/139‑1 (MQ=255)
                                                    tgCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACAcc                                                                                                                                      <  2:345812/87‑1 (MQ=255)
                                                    tgCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACAcc                                                                                                                                      >  1:345812/1‑87 (MQ=255)
                                                    tgCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGt                                                                                                                                 <  2:447450/92‑1 (MQ=255)
                                                    tgCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGt                                                                                                                                 >  1:447450/1‑92 (MQ=255)
                                                    tgCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCa                                                                                             <  1:817433/128‑1 (MQ=255)
                                                    tgCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGcc                                                                                  >  1:89551/1‑139 (MQ=255)
                                                     gCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCg                                                                                 <  2:252707/139‑1 (MQ=255)
                                                     gCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCg                                                                                 <  2:845380/139‑1 (MQ=255)
                                                        tCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTg                                                                              <  2:1333295/139‑1 (MQ=255)
                                                          aGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTggg                                                                            >  2:438316/1‑139 (MQ=255)
                                                              gcgcGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGt                                                                                                                                 >  1:1065911/1‑82 (MQ=255)
                                                              gcgcGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGt                                                                                                                                 <  2:1065911/82‑1 (MQ=255)
                                                              gcgcGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTaa                                                                                                                              <  1:1429889/85‑1 (MQ=255)
                                                               cgcgAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTaa                                                                                                                              <  1:1396509/84‑1 (MQ=255)
                                                               cgcgAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTaa                                                                                                                              >  2:1396509/1‑84 (MQ=255)
                                                               cgcgAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGt                                                                       <  1:1147929/139‑1 (MQ=255)
                                                                     cgcgGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTcc                                                                 <  2:89551/139‑1 (MQ=255)
                                                                         gAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAg                                                                                            <  2:871758/108‑1 (MQ=255)
                                                                         gAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAg                                                                                            >  1:871758/1‑108 (MQ=255)
                                                                               aCGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACAcc                                                                                                                                      <  1:1446741/60‑1 (MQ=255)
                                                                               aCGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGt                                                                                                                                 >  1:83263/1‑65 (MQ=255)
                                                                                 ggCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACAccc                                                                                                                                     <  2:578410/59‑1 (MQ=255)
                                                                                 ggCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACAccc                                                                                                                                     >  1:578410/1‑59 (MQ=255)
                                                                                       cTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGt                                                                                                                                 >  2:210636/1‑57 (MQ=255)
                                                                                       cTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTcc                                               >  2:458247/1‑139 (MQ=255)
                                                                                                  aTAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCAc                                    >  2:826827/1‑139 (MQ=255)
                                                                                                      aTGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGt                                                                                                                                 >  2:469568/1‑42 (MQ=255)
                                                                                                          ccccACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGc                            <  1:1443177/139‑1 (MQ=255)
                                                                                                          ccccACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGc                            <  1:939801/139‑1 (MQ=255)
                                                                                                          ccccACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGc                            <  2:126635/139‑1 (MQ=255)
                                                                                                           cccACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGt                                                                       >  2:409359/1‑95 (MQ=255)
                                                                                                               cGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCa                                                                                             >  2:916874/1‑69 (MQ=255)
                                                                                                               cGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCa                                                                                             <  1:916874/69‑1 (MQ=255)
                                                                                                               cGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGc                                                                                   >  1:733608/1‑79 (MQ=255)
                                                                                                               cGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGc                                                                                   <  2:733608/79‑1 (MQ=255)
                                                                                                               cGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTgggg                                                                           >  2:68509/1‑87 (MQ=255)
                                                                                                               cGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGc                       >  2:1022240/1‑139 (MQ=255)
                                                                                                                  aGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTaa                    <  1:99287/139‑1 (MQ=255)
                                                                                                                    aTCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGc                                                                                   <  1:1036116/74‑1 (MQ=255)
                                                                                                                     tCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTcc                                                                 >  1:351266/1‑91 (MQ=255)
                                                                                                                     tCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAg                 >  2:83272/1‑139 (MQ=255)
                                                                                                                        cTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCa                                                          >  1:1104117/1‑95 (MQ=255)
                                                                                                                          aaCACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCcg                                                                                                            >  2:1468790/1‑43 (MQ=255)
                                                                                                                            cacCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGTGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAGCGCAATc          >  1:1347784/1‑139 (MQ=255)
                                                                                                                              ccAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCgg                                             >  2:634513/1‑102 (MQ=255)
                                                                                                                              ccAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCgg                                             <  1:634513/102‑1 (MQ=255)
                                                                                                                               cAGGAAAACACCCCAGTTAATGTATTTCATGTCAcc                                                                                                              <  2:886525/36‑1 (MQ=255)
                                                                                                                               cAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCa                                                                                             >  1:96677/1‑53 (MQ=255)
                                                                                                                               cAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCgg                                             <  1:21915/101‑1 (MQ=255)
                                                                                                                               cAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAGCGCAATCaaa       >  1:40686/1‑139 (MQ=255)
                                                                                                                                 ggAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGa                                                                                                    <  1:436880/44‑1 (MQ=255)
                                                                                                                                 ggAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGc                            >  1:259575/1‑116 (MQ=255)
                                                                                                                                 ggAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGc                            <  2:129518/116‑1 (MQ=255)
                                                                                                                                 ggAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGc                            >  1:129518/1‑116 (MQ=255)
                                                                                                                                 ggAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGc                            <  2:259575/116‑1 (MQ=255)
                                                                                                                                 ggAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAGCGCAATCaaa       >  1:157493/1‑137 (MQ=255)
                                                                                                                                 ggAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAGCGCAATCAAAcc     >  1:891161/1‑139 (MQ=255)
                                                                                                                                 ggAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAGCGCAATCAAAcc     >  2:610311/1‑139 (MQ=255)
                                                                                                                                 ggAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAGCGCAATCAAAcc     >  1:1185482/1‑139 (MQ=255)
                                                                                                                                  gAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAGCGCAATCAAACCg    <  1:142196/139‑1 (MQ=255)
                                                                                                                                    aaaCACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAGCGCAATCAAACCGAt  <  2:384036/139‑1 (MQ=255)
                                                                                                                                    aaaCACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAGCGCAATCAAACCGAt  <  2:220382/139‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                      
TGGCACCGAAGCCCATCAGTACACCACCTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACGCCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAGCGCAATCAAACCGAT  >  NC_000913/2084637‑2084907

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑