Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,274,166 A→G K601K (AAA→AAG)  yejA → microcin C ABC transporter periplasmic binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,274,1660AG100.0% 237.2 / NA 66K601K (AAA→AAG) yejAmicrocin C ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (35/31);  total (35/31)

TGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGC  >  NC_000913/2274030‑2274297
                                                                                                                                        |                                                                                                                                   
tgtgGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCagcaagca                                                                                                                                   <  2:623705/139‑1 (MQ=255)
  ttttcaaTGGCGGAAGACCGTCCTGGCTGGTGGGAAAAATTCTCCCAGTTGGCCTGGCTCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAAAAAAGCAGCCAAACTGCCTTCCGCCAGCAAGCAgg                                                                                                                                 <  1:954133/133‑1 (MQ=255)
                      tctcGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAGAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTAc                                                                                                             >  2:893047/1‑139 (MQ=255)
                           ccTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGAt                                                                                                        >  1:91839/1‑139 (MQ=255)
                                             cccAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGt                                                                                      <  2:1234778/139‑1 (MQ=255)
                                             cccAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGt                                                                                      >  2:870550/1‑139 (MQ=255)
                                             cccAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGt                                                                                      <  2:64256/139‑1 (MQ=255)
                                               cAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAGAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGa                                                                                    <  1:893047/139‑1 (MQ=255)
                                                   cggccgTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATccc                                                                                <  1:1156904/139‑1 (MQ=255)
                                                      ccgTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTg                                                                                        <  1:676729/128‑1 (MQ=255)
                                                      ccgTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTg                                                                                        >  2:676729/1‑128 (MQ=255)
                                                                cATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCg                                                                   >  2:185324/1‑139 (MQ=255)
                                                                cATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCg                                                                   >  2:1175901/1‑139 (MQ=255)
                                                                           tCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGgaga                                                                                                                             <  1:1258330/70‑1 (MQ=255)
                                                                           tCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGgaga                                                                                                                             >  2:1258330/1‑70 (MQ=255)
                                                                            cGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGt                                                                                                 >  2:1120110/1‑97 (MQ=255)
                                                                            cGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGt                                                                                                 <  1:1120110/97‑1 (MQ=255)
                                                                            cGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGt                                                                                      <  1:967422/108‑1 (MQ=255)
                                                                            cGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGt                                                                                      >  2:967422/1‑108 (MQ=255)
                                                                             ggTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGga                                                                                                                               >  1:738224/1‑66 (MQ=255)
                                                                             ggTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGga                                                                                                                               <  2:738224/66‑1 (MQ=255)
                                                                             ggTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTgg                                                                                       >  2:270627/1‑106 (MQ=255)
                                                                             ggTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTgg                                                                                       <  1:270627/106‑1 (MQ=255)
                                                                             ggTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATccc                                                                                >  1:774723/1‑113 (MQ=255)
                                                                             ggTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATccc                                                                                <  2:774723/113‑1 (MQ=255)
                                                                             ggTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCa                                                      >  2:343624/1‑139 (MQ=255)
                                                                             ggTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCa                                                      >  1:205991/1‑139 (MQ=255)
                                                                                       ccTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCAt                                            >  2:626511/1‑139 (MQ=255)
                                                                                        cTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATc                                           >  2:382093/1‑139 (MQ=255)
                                                                                           gtggtATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTc                                                                                                <  2:10450/83‑1 (MQ=255)
                                                                                           gtggtATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTc                                                                                                >  1:10450/1‑83 (MQ=255)
                                                                                           gtggtATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTa                                                                         >  1:1407528/1‑106 (MQ=255)
                                                                                           gtggtATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTa                                                                         <  2:1407528/106‑1 (MQ=255)
                                                                                           gtggtATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTg                                        >  1:655244/1‑139 (MQ=255)
                                                                                              gtATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTTATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCaa                                     >  2:219847/1‑139 (MQ=255)
                                                                                               tATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTaaat                                                                                                                       <  2:530469/56‑4 (MQ=255)
                                                                                               tATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTaaat                                                                                                                       >  1:530469/1‑53 (MQ=255)
                                                                                               tATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGt                                                                                             <  2:601679/82‑1 (MQ=255)
                                                                                               tATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGt                                                                                             >  1:601679/1‑82 (MQ=255)
                                                                                                      tCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGAt                                                                                                        <  1:1283094/64‑1 (MQ=255)
                                                                                                      tCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGAt                                                                                                        >  2:1283094/1‑64 (MQ=255)
                                                                                                      tCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTg                                                                                           >  1:1212318/1‑77 (MQ=255)
                                                                                                      tCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTg                                                                                           <  2:1212318/77‑1 (MQ=255)
                                                                                                      tCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGt                                                                                      <  2:1390480/82‑1 (MQ=255)
                                                                                                      tCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGt                                                                                      >  1:1390480/1‑82 (MQ=255)
                                                                                                      tCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCa                                                         >  2:869019/1‑111 (MQ=255)
                                                                                                      tCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCa                                                         <  1:869019/111‑1 (MQ=255)
                                                                                                             aGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTg                                                                                           >  1:1094842/1‑70 (MQ=255)
                                                                                                             aGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTg                                                                                           <  2:1094842/70‑1 (MQ=255)
                                                                                                             aGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGcggt                      <  2:91839/139‑1 (MQ=255)
                                                                                                               cGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATggg                                                                     >  2:293857/1‑90 (MQ=255)
                                                                                                               cGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATggg                                                                     <  1:293857/90‑1 (MQ=255)
                                                                                                               cGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATgg                                                                      >  2:1016102/1‑89 (MQ=255)
                                                                                                               cGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATgg                                                                      <  1:1016102/89‑1 (MQ=255)
                                                                                                               cGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGcggtcc                    >  1:107490/1‑139 (MQ=255)
                                                                                                                 gCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATc                                                                                  >  2:788190/1‑75 (MQ=255)
                                                                                                                 gCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATc                                                                                  <  1:788190/75‑1 (MQ=255)
                                                                                                                 gCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGcggtccgg                  <  1:870550/139‑1 (MQ=255)
                                                                                                                  ccAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATggg                                                                                                                    <  1:97533/40‑1 (MQ=255)
                                                                                                                  ccAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATggg                                                                                                                    >  2:97533/1‑40 (MQ=255)
                                                                                                                  ccAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATgg                                                                                                                     >  2:434680/1‑39 (MQ=255)
                                                                                                                  ccAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATgg                                                                                                                     <  1:434680/39‑1 (MQ=255)
                                                                                                                          ccGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCg                                                                                                     >  1:1198193/1‑47 (MQ=255)
                                                                                                                          ccGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCg                                                                                                     <  2:1198193/47‑1 (MQ=255)
                                                                                                                                 ccAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATcgc  <  1:176459/139‑1 (MQ=255)
                                                                                                                                  cAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTg                                                                                     >  1:1365063/1‑55 (MQ=255)
                                                                                                                                  cAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTg                                                                                     <  2:1365063/55‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                   
TGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGC  >  NC_000913/2274030‑2274297

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑