Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,317,027	C→T	*950* (TAG→TAA)	rcsC ←	hybrid sensory kinase in two‑component regulatory system with RcsB and YojN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,317,027	0	C	T	100.0%	146.8 / NA	46	*950* (TAG→TAA)	rcsC	hybrid sensory kinase in two‑component regulatory system with RcsB and YojN
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/23);  total (23/23)

TTATCCGGCCTACGATTCCCATTATTTCAACAAATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCCTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCTGCTTCTCTTCAGCCAACG  >  NC_000913/2316904‑2317160                                                                                                                            |                                                                                                                                      ttATCCGGCCTACGATTCCCATTATTTCAACAAATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGAt                                                                                                                        >  1:174379/1‑139 (MQ=255)         ccTACGATTCCCATTATTTCAACAAATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACg                                                                                                                                   <  1:551195/120‑1 (MQ=255)         ccTACGATTCCCATTATTTCAACAAATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACg                                                                                                                                   >  2:551195/1‑120 (MQ=255)           tACGATTCCCATTATTTCAACAAATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGAccc                                                                                                              <  1:1254272/139‑1 (MQ=255)              gATTCCCATTATTTCAACAAATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCctct                                                                                                           <  1:701474/139‑1 (MQ=255)              gATTCCCATTATTTCAACAAATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCctct                                                                                                           <  1:878692/139‑1 (MQ=255)               aTTCCCATTATTTCAACAAATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCtctc                                                                                                          <  2:174379/139‑1 (MQ=255)                   ccATTATTTCAACAAATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTAc                                                                                                                                    <  2:1080207/109‑1 (MQ=255)                   ccATTATTTCAACAAATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTAc                                                                                                                                    >  1:1080207/1‑109 (MQ=255)                        attTCAACAAATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCCTTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAGTCCCGCGATTTCCTGACCCTCTCGGCATATaa                                                                                                 >  1:557722/1‑139 (MQ=255)                             aacaaATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCa                                                                                            >  1:515377/1‑139 (MQ=255)                                      cATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGTCTGtt                                                                                   <  1:379329/139‑1 (MQ=255)                                          aaaGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGTCTGTTTTAt                                                                               <  1:1213010/139‑1 (MQ=255)                                          aaaGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGTCTGTTTTAt                                                                               <  2:1247123/139‑1 (MQ=255)                                          aaaGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGTCTGTTTTAt                                                                               <  1:778879/139‑1 (MQ=255)                                                ggCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGTCTGTTTTATCACATc                                                                         >  2:1162028/1‑139 (MQ=255)                                                ggCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGTCTGTTTTATCACATc                                                                         >  2:1348435/1‑139 (MQ=255)                                                 gCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCctct                                                                                                           <  2:447439/104‑1 (MQ=255)                                                 gCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCctct                                                                                                           >  1:447439/1‑104 (MQ=255)                                                     gATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGTCTGTTTTATCACATCCAGCg                                                                    >  1:367668/1‑139 (MQ=255)                                                      aTAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTGTTACGAATCCCGCGATTTCCTGAc                                                                                                                <  2:897914/94‑1 (MQ=255)                                                      aTAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCcgcg                                                                                                                          <  1:324636/84‑1 (MQ=255)                                                      aTAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCcgcg                                                                                                                          >  2:324636/1‑84 (MQ=255)                                                      aTAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGAc                                                                                                                >  1:897914/1‑94 (MQ=255)                                                      aTAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCata                                                                                                    <  1:1472739/106‑1 (MQ=255)                                                      aTAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCata                                                                                                    >  2:1472739/1‑106 (MQ=255)                                                           aCGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGAccc                                                                                                              >  1:1286422/1‑91 (MQ=255)                                                           aCGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGAccc                                                                                                              <  2:1286422/91‑1 (MQ=255)                                                           aCGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCg                                                              >  1:176221/1‑139 (MQ=255)                                                                tCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCgg                                                                                                        <  1:237228/92‑1 (MQ=255)                                                                tCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCgg                                                                                                        >  2:237228/1‑92 (MQ=255)                                                                tCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTc                                                         >  2:394864/1‑139 (MQ=255)                                                                             tGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGt                                            <  1:1162028/139‑1 (MQ=255)                                                                              ggCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGTCTGTTTTa                                                                                >  2:557872/1‑102 (MQ=255)                                                                              ggCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGTCTGTTTTa                                                                                <  1:557872/102‑1 (MQ=255)                                                                              ggCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTc                                           >  2:1465964/1‑139 (MQ=255)                                                                                     gCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCg                                    <  2:367668/139‑1 (MQ=255)                                                                                                cGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATTCCGCGATTTCCTGAc                                                                                                                <  1:869617/52‑1 (MQ=18)                                                                                                cGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATTCCGCGATTTCCTGAc                                                                                                                >  2:869617/1‑52 (MQ=18)                                                                                                cGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGAccc                                                                                                              <  2:1111237/54‑1 (MQ=255)                                                                                                cGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGAccc                                                                                                              >  1:1111237/1‑54 (MQ=255)                                                                                                cGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGAc                                                                                                                <  2:1173115/52‑1 (MQ=255)                                                                                                cGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGAc                                                                                                                >  1:1173115/1‑52 (MQ=255)                                                                                                  gATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACAcc                       >  2:434971/1‑139 (MQ=255)                                                                                                                   ttATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCTGCTTCTCTTCAGcc      >  1:1299754/1‑139 (MQ=255)                                                                                                                       ccGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCTGCTTCTCTTCAGCCAACg  <  1:1465964/139‑1 (MQ=255)                                                                                                                            |                                                                                                                                      TTATCCGGCCTACGATTCCCATTATTTCAACAAATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCCTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCTGCTTCTCTTCAGCCAACG  >  NC_000913/2316904‑2317160

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑