Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,334,336 C→T pseudogene (67/4605 nt)
*208* (TAG→TAA
yfaS ←
yfaT ←
pseudogene, bacterial alpha2‑macroglobulin YfaS variant family, putative membrane protein
DUF1175 family protein, putative host defense protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,334,3360CT100.0% 122.2 / NA 38pseudogene (67/4605 nt)
*208* (TAG→TAA
yfaS
yfaT
pseudogene, bacterial alpha2‑macroglobulin YfaS variant family, putative membrane protein
DUF1175 family protein, putative host defense protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/16);  total (22/16)

CTGTCAGCAAGCAAAAAGAATGTTCCCCCGGCAGGCGGCGCATAGTTGCTGGAAGGAAGCGAATCATCAGCATTAGCAAGCCCTGTTCCCACAAGAGACAGGGACAAGCACGCTGCTATCGCGCCAGAAAATTTAAACGATAAATGCCAATGAAATTGGGATTGGAATCGTTGGGTATCCATCGGGTGTCCTTCCATGTCATAAGTTGTTGCAGACTGACTGCGCGCATTCCGTTGTCAGTTTTC  >  NC_000913/2334221‑2334465
                                                                                                                   |                                                                                                                                 
ctgTCAGCAAGCAAAAAGAATGTTCCCCCGGCAGGCGGCGCATAGTTGCTGGAAGGAAGCGAATCATCAGCATTAGCAAGCCCTGTTCCCACAAGAGACAGGGACAAGCACGCTGTTATCGCGCCAGAAAATTTAAACg                                                                                                            >  1:889376/1‑139 (MQ=255)
 tgTCAGCAAGCAAAAAGAATGTTCCCCCGGCAGGCGGCGCATAGTTGCTGGAAGGAAGCGAATCATCAGCATTAGCAAGCCCTGTTCCCACAAGAGACAGGGACAAGCACGCTGTTATCGCGCCAGAAAATTTAAACGa                                                                                                           >  2:761214/1‑139 (MQ=255)
               aaGAATGTTCCCCCGGCAGGCGGCGCATAGTTGCTGGAAGGAAGCGAATCATCAGCATTAGCAAGCCCTGTTCCCACAAGAGACAGGGACAAGCACGCTGTTATCGCGCCAGAAAATTTAAACGATAAATGCCAATGaa                                                                                             >  2:1155849/1‑139 (MQ=255)
                  aaTGTTCCCCCGGCAGGCGGCGCATAGTTGCTGGAAGGAAGCGAATCATCAGCATTAGCAAGCCCTGTTCCCACAAGAGACAGGGACAAGCACGCTGTTATCGCGCCAGAAAATTTAAACGATAAATGCCAATGAAAtt                                                                                          <  1:761214/139‑1 (MQ=255)
                            cGGCAGGCGGCGCATAGTTGCTGGAAGGAAGCGAATCATCAGCATTAGCAAGCCCTGTTCCCACAAGAGACAGGGACAAGCACGCTGTTATCGCGCCa                                                                                                                         >  1:1151353/1‑98 (MQ=255)
                            cGGCAGGCGGCGCATAGTTGCTGGAAGGAAGCGAATCATCAGCATTAGCAAGCCCTGTTCCCACAAGAGACAGGGACAAGCACGCTGTTATCGCGCCa                                                                                                                         <  2:1151353/98‑1 (MQ=255)
                              gCAGGCGGCGCATAGTTGCTGGAAGGAAGCGAATCATCAGCATTAGCAAGCCCTGTTCCCACAAGAGACAGGGACAAGCACGCTGTTATCGCGCCAGAAAATTTAAACGATAAATGCCAATGAAATTGGGATTGGAATc                                                                              >  1:411392/1‑139 (MQ=255)
                                aGGCGGCGCATAGTTGCTGGAAGGAAGCGAATCATCAGCATTAGCAAGCCCTGTTCCCACAAGAGACAGGGACAAGCACGCTGTTATCGCGCCAGAAAATTTAAACGATAAATGCCAATGAAAtt                                                                                          >  1:1130884/1‑125 (MQ=255)
                                aGGCGGCGCATAGTTGCTGGAAGGAAGCGAATCATCAGCATTAGCAAGCCCTGTTCCCACAAGAGACAGGGACAAGCACGCTGTTATCGCGCCAGAAAATTTAAACGATAAATGCCAATGAAAtt                                                                                          <  2:1130884/125‑1 (MQ=255)
                                            gTTGCTGGAAGGAAGCGAATCATCAGCATTAGCAAGCCCTGTTCCCACAAGAGACAGGGACAAGCACGCTGTTATCGCGCCAGAAAATTTAAACGATAAATGCCAATGAAATTGGGATTGGAATCGTTGGGTATCCATc                                                                <  2:411392/139‑1 (MQ=255)
                                                 tGGAAGGAAGCGAATCATCAGCATTAGCAAGCCCTGTTCCCACAAGAGACAGGGACAAGCACGCTGTTATCGCGCCAGAAAATTTAAACGATAAATGCCAATGAAATTGGGATTGGAATCGTTGGGTATCCATCGGgtg                                                           <  1:1155849/139‑1 (MQ=255)
                                                       gaagCGAATCATCAGCATTAGCAAGCCCTGTTCCCACAAGAGACAGGGACAAGCACGCTGTTATCGCGCCAGAAAATTTAAACGATAAATGCCAATGAAATTGGGATTGGAATCGTTggg                                                                        <  1:1067911/120‑1 (MQ=255)
                                                       gaagCGAATCATCAGCATTAGCAAGCCCTGTTCCCACAAGAGACAGGGACAAGCACGCTGTTATCGCGCCAGAAAATTTAAACGATAAATGCCAATGAAATTGGGATTGGAATCGTTggg                                                                        >  2:1067911/1‑120 (MQ=255)
                                                       gaagCGAATCATCAGCATTAGCAAGCCCTGTTCCCACAAGAGACAGGGACAAGCACGCTGTTATCGCGCCAGAAAATTTAAACGATAAATGCCAATGAAATTGGGATTGGAATCGTTGGGTATCCATCGGGTGTCCTTc                                                     <  2:889376/139‑1 (MQ=255)
                                                                     gCATTAGCAAGCCCTGTTCCCACAAGAGACAGGGACAAGCACGCTGTTATCGCGCCAGAAAATTTAAACGATAAATGCCAATGAAATTGGGATTGGAATCGTTGGGTATCCATCGGgtg                                                           <  2:398315/119‑1 (MQ=255)
                                                                     gCATTAGCAAGCCCTGTTCCCACAAGAGACAGGGACAAGCACGCTGTTATCGCGCCAGAAAATTTAAACGATAAATGCCAATGAAATTGGGATTGGAATCGTTGGGTATCCATCGGgtg                                                           >  1:398315/1‑119 (MQ=255)
                                                                        ttAGCAAGCCCTGTTCCCACAAGAGACAGGGACAAGCACGCTGTTATCGCGCCAGAAAATTTAAACGATAAATGCCAATGAAATTGGGATTGGAATCGTTGGGTATCCATCgg                                                              <  1:302455/113‑1 (MQ=255)
                                                                        ttAGCAAGCCCTGTTCCCACAAGAGACAGGGACAAGCACGCTGTTATCGCGCCAGAAAATTTAAACGATAAATGCCAATGAAATTGGGATTGGAATCGTTGGGTATCCATCgg                                                              >  2:302455/1‑113 (MQ=255)
                                                                           gCAAGCCCTGTTCCCACAAGAGACAGGGACAAGCACGCTGTTATCGCGCCAGAAAATTTAAACGATAAATGCCAATGAAATTGGGATTGGAATCGTTGGGTATCCATCGGGTGTCCTTCCATGTCATAAGTTGTTGCAg                                 >  2:332710/1‑139 (MQ=255)
                                                                            cAAGCCCTGTTCCCACAAGAGACAGGGACAAGCACGCTGTTATCGCGCCAGAAAATTTAAACGATAAATGCCAATGAAATTGGGATTGGAATCGTTGGGTATCCATCGGGTGTCCTTCCATGTCATAAGTTGTTGCAga                                >  1:581884/1‑139 (MQ=255)
                                                                              aGCCCTGTTCCCACAAGAGACAGGGACAAGCACGCTGTTATCGCGCCAGAAAATTTaaa                                                                                                              >  1:23122/1‑59 (MQ=255)
                                                                              aGCCCTGTTCCCACAAGAGACAGGGACAAGCACGCTGTTATCGCGCCAGAAAATTTaaa                                                                                                              <  2:23122/59‑1 (MQ=255)
                                                                              aGCCCTGTTCCCACAAGAGACAGGGACAAGCACGCTGTTATCGCGCCAGAAAATTTAAACGATAAATGCCAATGAAATTGGGATTGGAATCGTTGGGTATCCATCGGGTGTCCTTCCATGTCATAAGTTGTTGCAgact                              <  1:332710/139‑1 (MQ=255)
                                                                                cccTGTTCCCACAAGAGACAGGGACAAGCACGCTGTTATCGCGCCAGAAAATTTAAACGATAAATGCCAATGAAATTGGGATTGGAATCGTTGGGTATCCATCGGGTGTCCTTCCATGTCATAAg                                          <  1:713580/125‑1 (MQ=255)
                                                                                cccTGTTCCCACAAGAGACAGGGACAAGCACGCTGTTATCGCGCCAGAAAATTTAAACGATAAATGCCAATGAAATTGGGATTGGAATCGTTGGGTATCCATCGGGTGTCCTTCCATGTCATAAg                                          >  2:713580/1‑125 (MQ=255)
                                                                                cccTGTTCCCACAAGAGACAGGGACAAGCACGCTGTTATCGCGCCAGAAAATTTAAACGATAAATGCCAATGAAATTGGGATTGGAATCGTTGGGTATCCATCGGGTGTCCTTCCATGTCATAAGTTGTTGCAgactga                            >  1:460112/1‑139 (MQ=255)
                                                                                   tGTTCCCACAAGAGACAGGGACAAGCACGCTGTTATCGCGCCAGAAAATTTAAACGATAAATGCCAATGAAATTGGGATTGGAATCGTTGGGTATCCATCGGGTGTCCTTCCATGTCATAAGTTGTTGCAGactgactg                         >  1:885835/1‑139 (MQ=255)
                                                                                     ttCCCACAAGAGACAGGGACAAGCACGCTGTTATCGCGCCAGAAAATTTAAACGATAAATGCCAATGAAATTGGGATTGGAATCGTTGGGTATCCATCGGGTGTCCTTCCATGTCATAAGTTGTTGCAgactgcgcgca                       >  2:1299861/1‑133 (MQ=255)
                                                                                     ttCCCACAAGAGACAGGGACAAGCACGCTGTTATCGCGCCAGAAAATTTAAACGATAAATGCCAATGAAATTGGGATTGGAATCGTTGGGTATCCATCGGGTGTCCTTCCATGTCATAAGTTGTTGCAGACTGACTgcg                       >  1:1167106/1‑139 (MQ=255)
                                                                                       cccACAAGAGACAGGGACAAGCACGCTGTTATCGCGCCAGAAAATTTAAACGATAAATGCCAATGAAATTGGGATTGGAAt                                                                               <  1:106622/81‑1 (MQ=255)
                                                                                       cccACAAGAGACAGGGACAAGCACGCTGTTATCGCGCCAGAAAATTTAAACGATAAATGCCAATGAAATTGGGATTGGAAt                                                                               >  2:106622/1‑81 (MQ=255)
                                                                                                   aGGGACAAGCACGCTGTTATCGCGCCAGAAAATTTAAACGATAAATGCCAATGAAATTGGGATTGGAATCg                                                                             >  2:513448/1‑71 (MQ=255)
                                                                                                   aGGGACAAGCACGCTGTTATCGCGCCAGAAAATTTAAACGATAAATGCCAATGAAATTGGGATTGGAATCg                                                                             <  1:513448/71‑1 (MQ=255)
                                                                                                    gggACAAGCACGCTGTTATCGCGCCAGAAAATTTAAACGATAAATGcc                                                                                                   <  2:517814/48‑1 (MQ=255)
                                                                                                    gggACAAGCACGCTGTTATCGCGCCAGAAAATTTAAACGATAAATGcc                                                                                                   >  1:517814/1‑48 (MQ=255)
                                                                                                          aGCACGCTGTTATCGCGCCAGAAAATTTAAACGATAAATGCCAATGAAATTggg                                                                                       >  1:49584/1‑54 (MQ=255)
                                                                                                          aGCACGCTGTTATCGCGCCAGAAAATTTAAACGATAAATGCCAATGAAATTggg                                                                                       <  2:49584/54‑1 (MQ=255)
                                                                                                          aGCACGCTGTTATCGCGCCAGAAAATTTAAACGATAAATGCCAATGAAATTGGGATTGGAATCGTTGGGTATCCATCGGGTGTCCTTCCATGTCATAAGTTGTTGCAGACTGACTGCGCGCATTCCGTTGTCAGTTTTc  >  1:843019/1‑139 (MQ=255)
                                                                                                                ctgttATCGCGCCAGAAAATTTAAACGATAAATGCCAATGAAATTGGGATTGGAATCGTTGGGTATCCATc                                                                >  2:523141/1‑71 (MQ=255)
                                                                                                                ctgttATCGCGCCAGAAAATTTAAACGATAAATGCCAATGAAATTGGGATTGGAATCGTTGGGTATCCATc                                                                <  1:523141/71‑1 (MQ=255)
                                                                                                                   |                                                                                                                                 
CTGTCAGCAAGCAAAAAGAATGTTCCCCCGGCAGGCGGCGCATAGTTGCTGGAAGGAAGCGAATCATCAGCATTAGCAAGCCCTGTTCCCACAAGAGACAGGGACAAGCACGCTGCTATCGCGCCAGAAAATTTAAACGATAAATGCCAATGAAATTGGGATTGGAATCGTTGGGTATCCATCGGGTGTCCTTCCATGTCATAAGTTGTTGCAGACTGACTGCGCGCATTCCGTTGTCAGTTTTC  >  NC_000913/2334221‑2334465

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: