Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,462,834	T→C	intergenic (+188/‑178)	fadL → / → yfdF	long‑chain fatty acid outer membrane transporter/uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,462,834	0	T	C	100.0%	238.5 / NA	67	intergenic (+188/‑178)	fadL/yfdF	long‑chain fatty acid outer membrane transporter/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/38);  total (29/38)

CTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGG  >  NC_000913/2462702‑2462969                                                                                                                                     |                                                                                                                                        cTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGt                                                                                                                                   <  1:1213949/139‑1 (MQ=255)   tCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTcc                                                                                                                                 <  2:780854/139‑1 (MQ=255)                       aTTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTa                                                                                                                  <  2:947962/134‑1 (MQ=255)                       aTTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTa                                                                                                                  >  1:947962/1‑134 (MQ=255)                              aCCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCAtt                                                                                                      >  1:1307159/1‑139 (MQ=255)                                ctcctGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACtttt                                                                                                             >  1:674194/1‑130 (MQ=255)                                ctcctGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACtttt                                                                                                             <  2:674194/130‑1 (MQ=255)                                 tcctGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTcc                                                                                                   <  1:300222/139‑1 (MQ=255)                                   ctGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAg                                                                                                 >  1:878755/1‑139 (MQ=255)                                   ctGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAg                                                                                                 >  1:299945/1‑139 (MQ=255)                                    tGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGt                                                                                                                                   >  2:864511/1‑104 (MQ=255)                                    tGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGt                                                                                                                                   <  1:864511/104‑1 (MQ=255)                                     gAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCAt                                                                                                       >  2:317920/1‑131 (MQ=255)                                     gAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCAt                                                                                                       <  1:317920/131‑1 (MQ=255)                                               aaaaGCCTGTTGGCAGCTTGCACTTTCAAATCAATAAGCCGTATCCACAGACAAACCCGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTcccccc                                                                                     <  1:1394861/136‑1 (MQ=255)                                               aaCAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTcccccc                                                                                     <  2:790789/139‑1 (MQ=255)                                               aaCAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTcccccc                                                                                     <  2:878755/139‑1 (MQ=255)                                                           gCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGAC‑AACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATtgtg                                                                       <  2:276320/139‑1 (MQ=255)                                                            cAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGt                                                                                                                                   <  2:708590/80‑1 (MQ=255)                                                            cAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGt                                                                                                                                   >  1:708590/1‑80 (MQ=255)                                                            cAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATtgtg                                                                       <  2:371958/139‑1 (MQ=255)                                                              gCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCCAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTgcg                                                                     <  1:1435144/139‑1 (MQ=255)                                                              gCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCCTCCTTCTTAAAGAATTTACTTTTCTGGCTTTCCAGGATAC‑CCCCCCCCCTGGCTATTGTgcg                                                                     <  1:546605/139‑1 (MQ=255)                                                               cTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTgcgc                                                                    >  1:448160/1‑139 (MQ=255)                                                                 tGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATTTTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑CCCCCCCCCTGGCTATTGTGCGCTCa                                                                 <  2:856123/139‑1 (MQ=255)                                                                        tCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTcc                                                                                         >  1:25650/1‑110 (MQ=255)                                                                        tCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTcc                                                                                         <  2:25650/110‑1 (MQ=255)                                                                         cAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTc                                                          >  1:686142/1‑139 (MQ=255)                                                                         cAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTc                                                          <  2:448160/139‑1 (MQ=255)                                                                                aTAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTcc                                                                                                                                 >  1:28921/1‑62 (MQ=255)                                                                                aTAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTcc                                                                                                                                 <  2:28921/62‑1 (MQ=255)                                                                                 tAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTg                                                                                                          >  1:621860/1‑84 (MQ=255)                                                                                 tAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTg                                                                                                          <  2:621860/84‑1 (MQ=255)                                                                                    gCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTc                                                                                                    <  1:746479/87‑1 (MQ=255)                                                                                    gCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTc                                                                                                    >  2:746479/1‑87 (MQ=255)                                                                                    gCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                               <  2:1307159/139‑1 (MQ=255)                                                                                        tATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTcccccc                                                                                     >  2:1269411/1‑98 (MQ=255)                                                                                        tATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTcccccc                                                                                     <  1:1269411/98‑1 (MQ=255)                                                                                          tCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTcccccccctggc                                                                               <  1:775829/102‑5 (MQ=255)                                                                                          tCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTcccccccctggc                                                                               >  2:775829/1‑98 (MQ=255)                                                                                               aGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATacac                                                            >  1:1000618/1‑115 (MQ=255)                                                                                               aGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATacac                                                            <  2:1000618/115‑1 (MQ=255)                                                                                               aGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCtaaat                                    >  2:1231613/1‑139 (MQ=255)                                                                                                  cAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGc                                                                                                                                       <  2:429333/38‑1 (MQ=255)                                                                                                  cAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGc                                                                                                                                       >  1:429333/1‑38 (MQ=255)                                                                                                  cAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCAttt                                                                                                     >  1:314482/1‑72 (MQ=255)                                                                                                  cAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCAttt                                                                                                     <  2:314482/72‑1 (MQ=255)                                                                                                    aaCCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATg                               >  2:130310/1‑139 (MQ=255)                                                                                                     aCCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGc                                                                                                         >  2:502638/1‑65 (MQ=255)                                                                                                     aCCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGc                                                                                                         <  1:502638/65‑1 (MQ=255)                                                                                                      ccTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTAC‑TTTCTGCATTTCCAGGATACTcccccccc                                                                                   >  2:1368122/1‑85 (MQ=255)                                                                                                      ccTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAATTTAC‑TTTCTGCATTTCCAGGATACTcccccccc                                                                                   <  1:1368122/85‑1 (MQ=255)                                                                                                        tGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCa                                                                                                                           <  2:575654/44‑1 (MQ=255)                                                                                                        tGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCa                                                                                                                           >  1:575654/1‑44 (MQ=255)                                                                                                         gAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGt                          <  2:1349984/139‑1 (MQ=255)                                                                                                           aGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGc                                                                                                         >  1:1372864/1‑59 (MQ=255)                                                                                                           aGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGc                                                                                                         <  2:1372864/59‑1 (MQ=255)                                                                                                               aaaGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTcccccc                                                                                     >  1:573143/1‑75 (MQ=255)                                                                                                               aaaGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTcccccc                                                                                     <  2:573143/75‑1 (MQ=255)                                                                                                                aaGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGa                   <  2:299945/139‑1 (MQ=255)                                                                                                                aaGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGa                   <  1:1231613/139‑1 (MQ=255)                                                                                                                            cAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCa       <  1:130310/139‑1 (MQ=255)                                                                                                                             aGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGc                                                                                                         <  2:873650/41‑1 (MQ=255)                                                                                                                             aGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCCTGGCTATtgtg                                                                       >  1:182662/1‑75 (MQ=255)                                                                                                                             aGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCCTGGCTATtgtg                                                                       <  2:182662/75‑1 (MQ=255)                                                                                                                             aGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTAATTTTCTGc                                                                                                         >  1:873650/1‑41 (MQ=255)                                                                                                                                 gTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAgg  >  2:65040/1‑139 (MQ=255)                                                                                                                                     |                                                                                                                                        CTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGG  >  NC_000913/2462702‑2462969

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑