Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 220,239 C→T L230L (CTG→CTA)  metQ ← DL‑methionine transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913220,2390CT100.0% 160.4 / NA 50L230L (CTG→CTA) metQDL‑methionine transporter subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/26);  total (24/26)

GCCTAAAAATTACCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTGCCAGAGCGATTTGCGCGTCGTCCAGAGAACGCGGCAG  >  NC_000913/220104‑220370
                                                                                                                                       |                                                                                                                                   
gCCTAAAAATTACCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAgg                                                                                                                                  <  1:1428259/139‑1 (MQ=255)
 ccTAAAAATTACCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGt                                                                                                                                 >  1:896390/1‑139 (MQ=255)
  cTAAAAATTACCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGtt                                                                                                                                <  1:1364684/139‑1 (MQ=255)
             cAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACgggg                                                                                                                     <  1:70083/139‑1 (MQ=255)
                          cTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTc                                                                                                        >  2:375090/1‑139 (MQ=255)
                          cTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTc                                                                                                        >  1:632080/1‑139 (MQ=255)
                           tCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTca                                                                                                       >  1:795433/1‑139 (MQ=255)
                               ccgTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGAc                                                                                                                   <  2:888303/123‑1 (MQ=255)
                               ccgTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGAc                                                                                                                   >  1:888303/1‑123 (MQ=255)
                                  ttAAACACTTTGTTTTCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTc                                                                                                        <  1:1371740/131‑1 (MQ=255)
                                  ttAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTc                                                                                                        >  2:1371740/1‑131 (MQ=255)
                                            tgtttgCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATc                                                                                                           <  2:332104/118‑1 (MQ=255)
                                            tgtttgCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATc                                                                                                           >  1:332104/1‑118 (MQ=255)
                                                tgctgcTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGcc                                                                                  <  2:632080/139‑1 (MQ=255)
                                                    gctTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTtacgtacg                                                                                                                        >  2:636090/1‑97 (MQ=255)
                                                    gctTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTtacgtacg                                                                                                                        <  1:636090/97‑1 (MQ=255)
                                                    gctTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAg                                                                              >  1:600538/1‑139 (MQ=255)
                                                     ctTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGt                                                                             <  2:776912/139‑1 (MQ=255)
                                                        cGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAg                                                                          >  1:337213/1‑139 (MQ=255)
                                                                 cGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATctg                                                                 >  1:473562/1‑139 (MQ=255)
                                                                  gTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATctgg                                                                <  1:375090/139‑1 (MQ=255)
                                                                  gTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATctgg                                                                <  2:337213/139‑1 (MQ=255)
                                                                  gTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATctgg                                                                >  2:881540/1‑139 (MQ=255)
                                                                       aCTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCtggctggc                                                           <  2:754074/139‑1 (MQ=255)
                                                                       aCTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCtggctggc                                                           <  1:1464498/139‑1 (MQ=255)
                                                                         tGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCAt                                                         >  2:1115079/1‑139 (MQ=255)
                                                                           aTAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTtacgtac                                                                                                                         >  2:723560/1‑73 (MQ=255)
                                                                           aTAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTtacgtac                                                                                                                         <  1:723560/73‑1 (MQ=255)
                                                                           aTAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAg                                                       <  2:473562/139‑1 (MQ=255)
                                                                            tAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAgg                                                      <  2:600538/139‑1 (MQ=255)
                                                                                  tGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAggtg                                                    <  1:988755/135‑1 (MQ=255)
                                                                                  tGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCAGGCTGGCATAggtg                                                    >  2:988755/1‑135 (MQ=255)
                                                                                   ggACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACggg                                                                                                                      >  1:605026/1‑68 (MQ=255)
                                                                                   ggACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACggg                                                                                                                      <  2:605026/68‑1 (MQ=255)
                                                                                    gACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGcc                                                                                  <  2:262808/103‑1 (MQ=255)
                                                                                    gACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGcc                                                                                  >  1:262808/1‑103 (MQ=255)
                                                                                     aCGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTAt                                                >  2:482541/1‑136 (MQ=255)
                                                                                     aCGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTAt                                                <  1:482541/136‑1 (MQ=255)
                                                                                                      ctcGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAgg                                                      <  1:242622/113‑1 (MQ=255)
                                                                                                      ctcGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAgg                                                      >  2:242622/1‑113 (MQ=255)
                                                                                                         ggCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTGCCAGAGCGATTTgc                         <  1:281305/139‑1 (MQ=255)
                                                                                                                        ttCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATctgg                                                                >  2:1061440/1‑85 (MQ=255)
                                                                                                                        ttCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATctgg                                                                <  1:1061440/85‑1 (MQ=255)
                                                                                                                          cACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCAt                                                         >  1:9857/1‑90 (MQ=255)
                                                                                                                          cACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCAt                                                         <  2:9857/90‑1 (MQ=255)
                                                                                                                           aCGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAgg                                                      >  1:70698/1‑92 (MQ=255)
                                                                                                                           aCGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAgg                                                      <  2:70698/92‑1 (MQ=255)
                                                                                                                                tCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTGTTGATAACTGCCAGAGCGATTTGCGCGTCGTCCAGAGAACGCGGCAg  >  2:876762/1‑139 (MQ=255)
                                                                                                                                  aCGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAggtg                                                    <  2:195729/87‑1 (MQ=255)
                                                                                                                                  aCGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAggtg                                                    >  1:195729/1‑87 (MQ=255)
                                                                                                                                       |                                                                                                                                   
GCCTAAAAATTACCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTGCCAGAGCGATTTGCGCGTCGTCCAGAGAACGCGGCAG  >  NC_000913/220104‑220370

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑