Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,510,886	G→A	*419* (TAG→TAA)	yfeO →	putative ion channel protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,510,886	0	G	A	100.0%	206.9 / NA	64	*419* (TAG→TAA)	yfeO	putative ion channel protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (31/33);  total (31/33)

GCAGTCGTTGTACCCAATACCACATTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGAAGGAGAATAACAATGTTCAGGTCACTGTTT  >  NC_000913/2510752‑2511018                                                                                                                                       |                                                                                                                                     gcAGTCGTTGTACCCAATACCACATTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAACaaa                                                                                                                                  <  2:1251441/139‑1 (MQ=255)      cGTTGTACCCAATACCACATTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAACAAAAAATg                                                                                                                             <  1:589480/139‑1 (MQ=255)      cGTTGTACCCAATACCACATTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAACAAAAAATg                                                                                                                             <  2:760180/139‑1 (MQ=255)        ttGTACCCAATACCACATTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAACAAAAAATGct                                                                                                                           <  1:1003607/139‑1 (MQ=255)                aaTACCACATTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAACaaa                                                                                                                                  >  2:1224461/1‑124 (MQ=255)                aaTACCACATTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAACaaa                                                                                                                                  <  1:1224461/124‑1 (MQ=255)                aaTACCACATTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAACAAAAAATGCTCTCCTTTa                                                                                                                   <  1:1389682/139‑1 (MQ=255)                       caTTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAACAAAAAATGCTCTCCTTTATGATTTa                                                                                                            >  1:1197695/1‑139 (MQ=255)                       caTTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAACAAAAAATGCTCTCCTTTATGATTTa                                                                                                            >  2:217629/1‑139 (MQ=255)                          tGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAACAAAAAATGct                                                                                                                           <  2:147428/121‑1 (MQ=255)                          tGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAACAAAAAATGct                                                                                                                           >  1:147428/1‑121 (MQ=255)                                    gctCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAACaaa                                                                                                                                  <  2:50747/104‑1 (MQ=255)                                    gctCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAACaaa                                                                                                                                  >  1:50747/1‑104 (MQ=255)                                     ctctGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAATCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAACAAAAAATGCTCTCCTTTa                                                                                                                   <  1:255204/118‑1 (MQ=255)                                     ctctGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCAGCCCACGATAACGTTTAACAAAAAATGCTCTCCTTTATGATTTAAGAGGTATGGCgct                                                                                              >  1:868362/1‑137 (MQ=255)                                     ctctGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAACAAAAAATGCTCTCCTTTa                                                                                                                   >  2:255204/1‑118 (MQ=255)                                           aTCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAACAAAAAATGCTCTCCTTTa                                                                                                                   <  2:404595/112‑1 (MQ=255)                                           aTCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAACAAAAAATGCTCTCCTTTa                                                                                                                   >  1:404595/1‑112 (MQ=255)                                             cGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAACAAAAAATGCTCTCCTTTATGATTTAAGAGTTa                                                                                                     <  1:902168/124‑1 (MQ=255)                                             cGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAACAAAAAATGCTCTCCTTTATGATTTAAGAGTTa                                                                                                     >  2:902168/1‑124 (MQ=255)                                               tCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAACAAAAAATGCTCTCCTTTATGATTTAAGAGTTAt                                                                                                    <  2:1001674/123‑1 (MQ=255)                                               tCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAACAAAAAATGCTCTCCTTTATGATTTAAGAGTTAt                                                                                                    >  1:1001674/1‑123 (MQ=255)                                                  tGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAACAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCttt                                                                                 >  1:508546/1‑139 (MQ=255)                                                  tGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAACAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCttt                                                                                 >  1:731295/1‑139 (MQ=255)                                                  tGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAACAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCttt                                                                                 >  1:731346/1‑139 (MQ=255)                                                    cTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAACAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCga                                                                                               <  1:114834/123‑1 (MQ=255)                                                    cTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAACAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCga                                                                                               >  2:114834/1‑123 (MQ=255)                                                    cTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAACAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTAc                                                                               >  1:839283/1‑139 (MQ=255)                                                    cTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAACAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTAc                                                                               <  2:508546/139‑1 (MQ=255)                                                    cTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAACAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTAc                                                                               >  2:787051/1‑139 (MQ=255)                                                      tCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAACAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCt                                                                             <  2:731295/139‑1 (MQ=255)                                                      tCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAACAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCt                                                                             >  2:114474/1‑139 (MQ=255)                                                      tCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAACAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCt                                                                             <  2:839283/139‑1 (MQ=255)                                                      tCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAACAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCt                                                                             <  1:1173444/139‑1 (MQ=255)                                                        cGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAACAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTcc                                                                           <  1:217629/139‑1 (MQ=255)                                                        cGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAACAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTcc                                                                           <  1:994640/139‑1 (MQ=255)                                                         ggCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAACAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTccc                                                                          <  1:787051/139‑1 (MQ=255)                                                               gCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAACAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAat                                                                    <  1:114474/139‑1 (MQ=255)                                                                       taGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAACAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGtt                                                            >  1:318808/1‑139 (MQ=255)                                                                         gCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAACAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTaa                                                          >  2:958582/1‑139 (MQ=255)                                                                          cAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAACAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAAc                                                         >  1:1083449/1‑139 (MQ=255)                                                                             gTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAACAAAAAATGct                                                                                                                           <  1:1427600/70‑1 (MQ=255)                                                                             gTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAACAAAAAATGct                                                                                                                           >  2:1427600/1‑70 (MQ=255)                                                                             gTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAACAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTcc                                                                           <  2:1371434/118‑1 (MQ=255)                                                                             gTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAACAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTcc                                                                           >  1:1371434/1‑118 (MQ=255)                                                                             gTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAACAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAgg                                                      >  1:309466/1‑139 (MQ=255)                                                                             gTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAACAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAgg                                                      >  2:68125/1‑139 (MQ=255)                                                                             gTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAACAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAgg                                                      <  1:1447629/139‑1 (MQ=255)                                                                                aGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAACAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCg                                                   >  2:1247414/1‑139 (MQ=255)                                                                                 gCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAACAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCt                                                                             <  1:1250387/112‑1 (MQ=255)                                                                                 gCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAACAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCt                                                                             >  2:1250387/1‑112 (MQ=255)                                                                                            tgGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAACAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCg                                       >  2:198268/1‑139 (MQ=255)                                                                                             ggTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAACAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGa                                      <  2:731346/139‑1 (MQ=255)                                                                                                   tCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAACAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGa                                <  2:318808/139‑1 (MQ=255)                                                                                                     gTCCGAAGCAACAGCCACCCCACGATAACGTTTAACAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGAAg                              <  1:1247414/139‑1 (MQ=255)                                                                                                     gTCCGAAGCAACAGCCACCCCACGATAACGTTTAACAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGAAg                              <  1:680644/139‑1 (MQ=255)                                                                                                     gTCCGAAGCAACAGCCACCCCACGATAACGTGTAACAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGAAg                              <  1:68125/139‑1 (MQ=255)                                                                                                        cGAAGCAACAGCCACCCCACGATAACGTTTAACAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGa                                <  1:768320/134‑1 (MQ=255)                                                                                                        cGAAGCAACAGCCACCCCACGATAACGTTTAACAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGa                                >  2:768320/1‑134 (MQ=255)                                                                                                                     aCCCCACGATAACGTTTAACAAAAAATGCTCTCCTTTATGAttt                                                                                                             >  2:697206/1‑44 (MQ=255)                                                                                                                     aCCCCACGATAACGTTTAACAAAAAATGCTCTCCTTTATGAttt                                                                                                             <  1:697206/44‑1 (MQ=255)                                                                                                                        ccACGATAACGTTTAACAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGAAGGAGAATAACAATGTTCAgg           <  2:309466/139‑1 (MQ=255)                                                                                                                             aTAACGTTTAACAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGAAGGAGAATAACAATGTTCAGGTCACt      >  1:908608/1‑139 (MQ=255)                                                                                                                                 cGTTTAACAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGAAGGAGAATAACAATGTTCAGGTCACTGttt  >  2:29983/1‑139 (MQ=255)                                                                                                                                       |                                                                                                                                     GCAGTCGTTGTACCCAATACCACATTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGAAGGAGAATAACAATGTTCAGGTCACTGTTT  >  NC_000913/2510752‑2511018

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑