Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,519,189	T→C	I126T (ATT→ACT)	yfeD →	DUF1323 family putative DNA‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,519,189	0	T	C	100.0%	204.5 / NA	58	I126T (ATT→ACT)	yfeD	DUF1323 family putative DNA‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/29);  total (29/29)

TACGCTCCTGGAGCACGCGCCCCGGCGTATCGACAAATCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGG  >  NC_000913/2519053‑2519320                                                                                                                                         |                                                                                                                                    tACGCTCCTGGAGCACGCGCCCCGGCGTATCGACAAATCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATACTg                                                                                                                                   >  1:527378/1‑139 (MQ=255)      tCCTGGAGCACGCGCCCCGGCGTATCGACAAATCATCAACGCGATAGATAATATGACAGGCATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATACTGACGaa                                                                                                                              >  2:1064360/1‑139 (MQ=255)                cgcgCCCCGGCGTATCGACAAATCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATACTGACGAATCAGCataaa                                                                                                                    >  2:870915/1‑139 (MQ=255)                cgcgCCCCGGCGTATCGACAAATCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATACTGACGAATCAGCataaa                                                                                                                    >  1:797994/1‑139 (MQ=255)                cgcgCCCCGGCGTATCGACAAATCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATACTGACGAATCAGCataaa                                                                                                                    >  1:191659/1‑139 (MQ=255)                cgcgCCCCGGCGTATCGACAAATCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATACTGACGAATCAGCataaa                                                                                                                    >  1:883814/1‑139 (MQ=255)                        ggCGTATCGACAAATCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATACTGACGAATc                                                                                                                            <  1:925342/123‑1 (MQ=255)                        ggCGTATCGACAAATCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATACTGACGAATc                                                                                                                            >  2:925342/1‑123 (MQ=255)                          cGTATCGACAAATCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATACTGACGAATCAGCATAAATAAAAAACgg                                                                                                          <  2:797994/139‑1 (MQ=255)                          cGTATCGACAAATCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATACTGACGAATCAGCATAAATAAAAAACgg                                                                                                          <  2:883814/139‑1 (MQ=255)                           gTATCGAAAAATCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATACTGACGAATCAGCATAAATAAAAAACGGc                                                                                                         <  1:870915/139‑1 (MQ=255)                            tATCGACAAATCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTTGCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATACTGACGAATCAGCATAAATAAAAAACGGCa                                                                                                        <  2:718616/139‑1 (MQ=255)                              tCGACAAATCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATACTGACGAATCAGCATAAATAAAAAACGGCAgg                                                                                                      <  2:657105/139‑1 (MQ=255)                                           aaCGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATACTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTg                                                                                         <  1:13245/139‑1 (MQ=255)                                           aaCGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATACTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTg                                                                                         <  2:527378/139‑1 (MQ=255)                                            aCGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATACTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGc                                                                                        >  2:217162/1‑139 (MQ=255)                                                gatagataATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATACTGACGAATCAGCATACATAAAAAACGGCAGGATATTATCTCCTGCCGtt                                                                                    >  2:1062560/1‑139 (MQ=255)                                                gatagataATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATACTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGtt                                                                                    <  2:211142/139‑1 (MQ=255)                                                 atagataATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATACTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGttt                                                                                   >  2:384265/1‑139 (MQ=255)                                                          atGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATACTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTa                                                                          <  1:746376/139‑1 (MQ=255)                                                           tGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATACTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTac                                                                         <  1:493505/139‑1 (MQ=255)                                                           tGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATACTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTac                                                                         <  2:33825/139‑1 (MQ=255)                                                              cagacaTTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATACTGACGAATCAGCATAAATAAAAAACGGCAgg                                                                                                      >  1:629527/1‑107 (MQ=255)                                                              cagacaTTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATACTGACGAATCAGCATAAATAAAAAACGGCAgg                                                                                                      <  2:629527/107‑1 (MQ=255)                                                              cagacaTTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATACTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACg                                                                      >  1:448978/1‑139 (MQ=255)                                                              cagacaTTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATACTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACg                                                                      >  1:1468380/1‑139 (MQ=255)                                                               agacaTTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATACTGACGAATCAGCATAAATAAAAAACGGCa                                                                                                        >  1:655464/1‑104 (MQ=255)                                                               agacaTTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATACTGACGAATCAGCATAAATAAAAAACGGCa                                                                                                        <  2:655464/104‑1 (MQ=255)                                                               agacaTTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATACTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTcc                                                                                           <  1:184694/117‑1 (MQ=255)                                                               agacaTTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATACTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTcc                                                                                           >  2:184694/1‑117 (MQ=255)                                                               agacaTTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATACTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGc                                                                     >  2:148224/1‑139 (MQ=255)                                                               agacaTTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATACTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGc                                                                     >  1:478016/1‑139 (MQ=255)                                                                gacaTTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATACTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCt                                                                    >  2:1033023/1‑139 (MQ=255)                                                                 acaTTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATACTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTa                                                                   >  2:369154/1‑139 (MQ=255)                                                                   aTTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATACTGACGAATCAGCATAAATAAAAAACGGc                                                                                                         >  1:205335/1‑99 (MQ=255)                                                                   aTTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATACTGACGAATCAGCATAAATAAAAAACGGc                                                                                                         <  2:205335/99‑1 (MQ=255)                                                                   aTTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATACTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAAt                                                                 >  1:1002779/1‑139 (MQ=255)                                                                    ttGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATACTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTac                                                                         >  1:1065871/1‑130 (MQ=255)                                                                    ttGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATACTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTac                                                                         <  2:1065871/130‑1 (MQ=255)                                                                                 aGTCGCGCAATTTTTGTCACGTGAAGCAATTCGCAATTTCCTCGCCCGTCTCGATACTGACTATTCAGCATAAATAAAAAACGGGAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTACTGATTTTc                                                   >  1:1176523/1‑139 (MQ=255)                                                                                    cgcgCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATACTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTcgcg                                                <  2:478016/139‑1 (MQ=255)                                                                                      cgcAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATACTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGtt                                              >  2:1328006/1‑139 (MQ=255)                                                                                      cgcAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATACTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGtt                                              <  1:217162/139‑1 (MQ=255)                                                                                           tttttGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATACTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCa                                         <  1:1328006/139‑1 (MQ=255)                                                                                                gTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATACTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATaaa                                    >  1:1076378/1‑139 (MQ=255)                                                                                                      tGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATACTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTa                                                               <  1:860001/106‑1 (MQ=255)                                                                                                      tGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATACTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTa                                                               >  2:860001/1‑106 (MQ=255)                                                                                                        aaGGAATTCGCAATTTCCTCGCCCGTCTCGATACTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAgcg                            <  1:384265/139‑1 (MQ=255)                                                                                                        aaGGAATTCGCAATTTCCTCGCCCGTCTCGATACTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAgcg                            <  2:191659/139‑1 (MQ=255)                                                                                                        aaGGAATTCGCAATTTCCTCGCCCGTCTCGATACTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAgcg                            <  1:148224/139‑1 (MQ=255)                                                                                                        aaGGAATTCGCAATTTCCTCGCCCGTCTCGATACTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAgcg                            <  2:1076378/139‑1 (MQ=255)                                                                                                        aaGGAATTCGCAATTTCCTCGCCCGTCTCGATACTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAgcg                            <  1:1064360/139‑1 (MQ=255)                                                                                                                cGCAATTTCCTCGCCCGTCTCGATACTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTcgcg                                                <  1:626556/111‑1 (MQ=255)                                                                                                                cGCAATTTCCTCGCCCGTCTCGATACTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTcgcg                                                >  2:626556/1‑111 (MQ=255)                                                                                                                cGCAATTTCCTCGCCCGTCTCGATACTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTttgttg                    >  2:841122/1‑139 (MQ=255)                                                                                                                                 tctcGATACTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTac                                                                         <  1:1462676/69‑1 (MQ=255)                                                                                                                                 tctcGATACTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTac                                                                         >  2:1462676/1‑69 (MQ=255)                                                                                                                                  ctcGATACTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCgg  <  2:1002779/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                                    TACGCTCCTGGAGCACGCGCCCCGGCGTATCGACAAATCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGG  >  NC_000913/2519053‑2519320

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑