Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,574,135 G→A D57D (GAC→GAT eutT ← cobalamin adenosyltransferase involved in ethanolamine utilization

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,574,1350GA100.0% 111.4 / NA 35D57D (GAC→GATeutTcobalamin adenosyltransferase involved in ethanolamine utilization
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/17);  total (18/17)

TTTTCCGCCGACAGGTGAGTCAGCGTATCCGGCTTTTTTGCCACCGGCTGGCGACACAGTTCGCAGCACGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCGTCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGATCTCTGCGCCTTCGCTGAGCGTATGGTT  >  NC_000913/2574004‑2574269
                                                                                                                                   |                                                                                                                                      
ttttGCGCCAACAGGTGAGTCAGCGGATCCGGATTTTTTGCCACCGGCTGGCGACACAGTTCGCAGCACGCCTGCGGATGTTCTTCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATcaacaa                                                                                                                                 <  1:77444/139‑1 (MQ=255)
      gccgACAGGTGAGTCAGCGTATCCGGCTTTTTTGCCACCGGCTGGCGACACAGTTCGCAGCACGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAggc                                                                                                                           >  2:1016625/1‑139 (MQ=255)
               tGAGTCAGCGTATCCGGCTTTTTTGCCACCGGCTGGCGACACAGTTCGCAGCACGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCt                                                                                                                  <  1:1016625/139‑1 (MQ=255)
                                           ccGGCTGGCGACACAGTTCGCAGCACGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATggcg                                                                                      <  2:572889/139‑1 (MQ=255)
                                                          gTTCGCAGCACGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTc                                                                       >  2:533573/1‑139 (MQ=255)
                                                               cagcaCGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCggg                                                                  <  2:409011/139‑1 (MQ=255)
                                                                 gcaCGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCa                                                                <  2:606630/139‑1 (MQ=255)
                                                                  caCGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCag                                                               <  1:533573/139‑1 (MQ=255)
                                                                     gCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAgg                                                            >  2:1365972/1‑139 (MQ=255)
                                                                      ccTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAggg                                                           <  2:270562/139‑1 (MQ=255)
                                                                       cTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGc                                                          >  2:783581/1‑139 (MQ=255)
                                                                       cTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGc                                                          >  1:555312/1‑139 (MQ=255)
                                                                                ttCATCGCTACTGGTCAGCCCATGAACGGGCTGCG‑‑‑GCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGt                                                                                                               >  1:493892/1‑74 (MQ=255)
                                                                                ttCATCGCTACTGGTCAGCCCATGAACGGGCTGCG‑‑‑GCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGt                                                                                                               <  2:493892/74‑1 (MQ=255)
                                                                                   aTCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTg                                              >  2:553350/1‑139 (MQ=255)
                                                                                      gCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTcc                                           >  2:1223965/1‑139 (MQ=255)
                                                                                          cTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCAg                                       <  2:1319474/139‑1 (MQ=255)
                                                                                                  cccATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTg                                                                                                                    >  1:164516/1‑54 (MQ=255)
                                                                                                  cccATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTg                                                                                                                    <  2:164516/54‑1 (MQ=255)
                                                                                                  cccATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCt                                                                                                                  <  1:1148651/56‑1 (MQ=255)
                                                                                                  cccATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCt                                                                                                                  >  2:1148651/1‑56 (MQ=255)
                                                                                                  cccATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATgg                               <  1:171219/139‑1 (MQ=255)
                                                                                                       gAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGcc                                                                                                                       <  2:1206793/46‑1 (MQ=255)
                                                                                                       gAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGcc                                                                                                                       >  1:1206793/1‑46 (MQ=255)
                                                                                                       gAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCaa                                                                          <  1:821062/91‑1 (MQ=255)
                                                                                                       gAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCaa                                                                          >  2:821062/1‑91 (MQ=255)
                                                                                                       gAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGAtctc                          >  1:987481/1‑139 (MQ=255)
                                                                                                            ggctgcggctgcTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCa                                                                                             >  2:1402072/1‑67 (MQ=255)
                                                                                                            ggctgcggctgcTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCa                                                                                             <  1:1402072/67‑1 (MQ=255)
                                                                                                                 cggctgcTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCAg                                       >  1:67012/1‑116 (MQ=255)
                                                                                                                 cggctgcTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCAg                                       <  2:67012/116‑1 (MQ=255)
                                                                                                                  ggctgcTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGATCTCTGCGCCTTCGc               >  2:114940/1‑139 (MQ=255)
                                                                                                                       ctgctgTTCATCATCAACAAACAGGCGGCCCTGCt                                                                                                                  <  2:330514/35‑1 (MQ=255)
                                                                                                                       ctgctgTTCATCATCAACAAACAGGCGGCCCTGCt                                                                                                                  >  1:330514/1‑35 (MQ=255)
                                                                                                                       ctgctgTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGATCTCTGCGCCTTCGCTGGGc          >  2:953697/1‑139 (MQ=255)
                                                                                                                              tcatcatcaACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGATCTCTGCGCCTTCGCTGAGCGTATGGt   <  1:114940/139‑1 (MQ=255)
                                                                                                                               catcatCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATggcg                                                                                      <  1:907829/55‑1 (MQ=255)
                                                                                                                               catcatCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATggcg                                                                                      >  2:907829/1‑55 (MQ=255)
                                                                                                                               catcatCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGATCTCTGCGCCTTCGCTGAGCGTATGGtt  <  1:990877/139‑1 (MQ=255)
                                                                                                                               catcatCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGATCTCTGCGCCTTCGCTGAGCGTATGGtt  <  1:553350/139‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                      
TTTTCCGCCGACAGGTGAGTCAGCGTATCCGGCTTTTTTGCCACCGGCTGGCGACACAGTTCGCAGCACGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCGTCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGATCTCTGCGCCTTCGCTGAGCGTATGGTT  >  NC_000913/2574004‑2574269

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: