Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,713,568 T→C W225R (TGG→CGG)  srmB → ATP‑dependent RNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,713,5680TC100.0% 152.3 / NA 43W225R (TGG→CGG) srmBATP‑dependent RNA helicase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/23);  total (20/23)

ACCCTGCTCTTTTCGGCAACGCTGGAAGGCGATGCGATTCAGGACTTTGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGTGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGACCCGCTCAATTGTGTTTGTGCGTAAGCGTGAGCGTGTGCATGAGCTGGCAAAC  >  NC_000913/2713445‑2713693
                                                                                                                           |                                                                                                                             
aCCCTGCTCTTTTCGGCAACGCTGGAAGGCGATGCGATTCAGGACTTTGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCg                                                                                                                >  2:1421858/1‑139 (MQ=255)
aCCCTGCTCTTTTCGGCAACGCTGGAAGGCGATGCGATTCAGGACTTTGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCg                                                                                                                >  2:1456718/1‑139 (MQ=255)
                       ggAAGGCGATGCGATTCAGGACTTTGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACcgcg                                                                                         <  2:161676/139‑1 (MQ=255)
                           ggCGATGCGATTCAGGACTTTGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGc                                                                                     <  1:1456718/139‑1 (MQ=255)
                                tgcgatTCAGGACTTTGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTg                                                                                <  1:491591/139‑1 (MQ=255)
                                  cgatTCAGGACTTTGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCa                                                                              <  1:741052/139‑1 (MQ=255)
                                      tCAGGACTTTGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCgcgc                                                                                                                  >  2:719985/1‑99 (MQ=255)
                                      tCAGGACTTTGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCgcgc                                                                                                                  <  1:719985/99‑1 (MQ=255)
                                            cTTTGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTaaaa                                                                    <  2:755973/139‑1 (MQ=255)
                                               tGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACcgc                                                                                                                    >  2:796954/1‑88 (MQ=255)
                                               tGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACcgc                                                                                                                    <  1:796954/88‑1 (MQ=255)
                                               tGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACcg                                                                                           >  2:538146/1‑113 (MQ=255)
                                               tGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACcg                                                                                           <  1:538146/113‑1 (MQ=255)
                                               tGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAg                                                                 >  1:1458066/1‑139 (MQ=255)
                                               tGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGGTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAg                                                                 >  2:120850/1‑139 (MQ=255)
                                                           gctgGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGAcc                                                     >  1:738938/1‑139 (MQ=255)
                                                                aaGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGACCCGCTc                                                <  1:1421858/139‑1 (MQ=255)
                                                                     ccGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCa                                                                              >  1:917917/1‑104 (MQ=255)
                                                                     ccGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCa                                                                              <  2:917917/104‑1 (MQ=255)
                                                                          ggAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGACCCGCTCAATTGTGttt                                      <  1:659712/139‑1 (MQ=255)
                                                                              gTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCa                                                                              >  1:770027/1‑95 (MQ=255)
                                                                              gTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCa                                                                              >  1:1405406/1‑95 (MQ=255)
                                                                              gTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCa                                                                              <  2:770027/95‑1 (MQ=255)
                                                                              gTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCa                                                                              <  2:1405406/95‑1 (MQ=255)
                                                                                ttCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGACCCGCTCAATTGTGTTTGTGCGt                                <  2:738938/139‑1 (MQ=255)
                                                                                             tCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGc                                                                                     >  1:778618/1‑73 (MQ=255)
                                                                                             tCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGc                                                                                     <  2:778618/73‑1 (MQ=255)
                                                                                               cACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACcgcg                                                                                         <  1:4008/67‑1 (MQ=255)
                                                                                               cACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACcgcg                                                                                         >  2:4008/1‑67 (MQ=255)
                                                                                                 cccGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGACCCGCTCAATTGTGTTTGTGCGTAAGCGTGAGCGTGTGCa               <  2:963297/139‑1 (MQ=255)
                                                                                                   cGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGACCCGCTCAATTGTGTTTGTGCGTAAGCGTGAGCGTGTGCATg             <  2:782955/139‑1 (MQ=255)
                                                                                                    gTGAGCGCAAAAAAATTCATCAGCGGTATTACCgcgc                                                                                                                  <  1:927756/37‑1 (MQ=255)
                                                                                                    gTGAGCGCAAAAAAATTCATCAGCGGTATTACCgcgc                                                                                                                  >  2:927756/1‑37 (MQ=255)
                                                                                                     tGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGACCCGCTCAATTGTGTTTGTGCGTAAGCGTGAGCGTGTGCATGAg           >  1:1100496/1‑139 (MQ=255)
                                                                                                       aGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGACCCGCTCAATTGTGTTTGTGCGTAagcgt                          >  2:1275776/1‑122 (MQ=255)
                                                                                                       aGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGACCCGCTCAATTGTGTTTGTGCGTAagcgt                          <  1:1275776/122‑1 (MQ=255)
                                                                                                         cgcAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGACCCGCTCAATTGTGTTTGTGCGTAAGCGTGAGCGTGTGCATGAGCTgg       >  2:253335/1‑139 (MQ=255)
                                                                                                         cgcAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGACCCGCTCAATTGTGTTTGTGCGTAAGCGTGAGCGTGTGCATGAGCTgg       >  2:823537/1‑139 (MQ=255)
                                                                                                           cAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCa                                                                              >  1:839252/1‑66 (MQ=255)
                                                                                                           cAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCa                                                                              <  2:839252/66‑1 (MQ=255)
                                                                                                            aaaaaaaTTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGACCCGCTCAATTGTGTTTGTGCGt                                >  1:1147528/1‑111 (MQ=255)
                                                                                                            aaaaaaaCTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGACCCGCTCAATTGTGTTTGTGCGt                                <  2:1147528/111‑1 (MQ=255)
                                                                                                              aaaaaTTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGACCCGCTCAATTGTGTTTGTGCGTAAGCGTGAGCGTGTGCATGAGCTGGCAAAc  <  2:1292006/139‑1 (MQ=255)
                                                                                                                           |                                                                                                                             
ACCCTGCTCTTTTCGGCAACGCTGGAAGGCGATGCGATTCAGGACTTTGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGTGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGACCCGCTCAATTGTGTTTGTGCGTAAGCGTGAGCGTGTGCATGAGCTGGCAAAC  >  NC_000913/2713445‑2713693

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑