Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,781,132	T→C	D532G (GAC→GGC)	ypjA ←	adhesin‑like autotransporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,781,132	0	T	C	100.0%	161.7 / NA	46	D532G (GAC→GGC)	ypjA	adhesin‑like autotransporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/23);  total (23/23)

CATTTTTAGCTTCTGAATTCCTGATGTAGAAAGCATCACCATAACTGTTGGTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGTCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTGGACAATCAGCTTACCGCCAGTATCAATGGTAGTATTTTTCGCCGAGGTTTTAGCCACTACCGTCAGTTCG  >  NC_000913/2780998‑2781263                                                                                                                                       |                                                                                                                                    cATTTTTAGCTTCTGAATTCCTGATGTAGAAAGCATCACCATAACTGTTGGTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTg                                                                                                                                 >  2:662993/1‑139 (MQ=255) cATTTTTAGCTTCTGAATTCCTGATGTAGAAAGCATCACCATAACTGTTGGTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTg                                                                                                                                 >  2:1267316/1‑139 (MQ=255)        aGCTTCTGAATTCCTGATGTAGAAAGCATCACCATAACTGTTGGTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTcc                                                                                                                          <  1:653963/139‑1 (MQ=255)            tCTGAATTCCTGATGTAGAAAGCATCACCATAACTGTTGGTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTAAACATGCTTAGCCTCACAACCGCCCTAAACCTCCAGaa                                                                                                                      >  1:69646/1‑139 (MQ=255)               gAATTCCTGATGTAGAAAGCATCACCATAACTGTTGGTTCCTTCGATAAGTGTTCCGGAGGCCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAAcgc                                                                                                                   <  1:931569/139‑1 (MQ=255)                  ttCCTGATGTAGAAAGCATCACCATAACTGTTGGTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAAcgccgc                                                                                                                <  2:362131/139‑1 (MQ=255)                   tCCTGATGTAGAAAGCATCACCATAACTGTTGGTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACgccgcc                                                                                                               <  2:660248/139‑1 (MQ=255)                       gATGTAGAAAGCATCACCATAACTGTTGTTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCatta                                                                                                           >  2:1418593/1‑139 (MQ=255)                                  cATCACCATAACTGTTGGTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGt                                                                                                <  1:403097/139‑1 (MQ=255)                                    tCACCATAACTGTTGGTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTc                                                                                                    <  1:885950/133‑1 (MQ=255)                                    tCACCATAACTGTTGGTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCACAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTc                                                                                                    >  2:885950/1‑133 (MQ=255)                                     cACCATAACTGTTGGTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAAcgccg                                                                                                                 >  2:565666/1‑119 (MQ=255)                                     cACCATAACTGTTGGTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAAcgccg                                                                                                                 <  1:565666/119‑1 (MQ=255)                                     cACCATAACTGTTGGTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCt                                                                                             >  1:998234/1‑139 (MQ=255)                                          tAACTGTTGGTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTg                                                                                        >  2:428965/1‑139 (MQ=255)                                                ttGGTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAg                                                                                  >  1:107334/1‑139 (MQ=255)                                                  ggTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGcc                                                                                <  2:107334/139‑1 (MQ=255)                                                  ggTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGcc                                                                                >  1:928638/1‑139 (MQ=255)                                                     tCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACgccgcc                                                                                                               <  2:1351010/105‑1 (MQ=255)                                                     tCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACgccgcc                                                                                                               >  1:1351010/1‑105 (MQ=255)                                                      ccTTCGATAAGTGTTCTGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGccacct                                                                            <  2:998234/139‑1 (MQ=255)                                                      ccTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGcctcct                                                                            >  2:391894/1‑139 (MQ=255)                                                        ttCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTc                                                                          >  2:1208307/1‑139 (MQ=255)                                                                 tgtTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTGGACAATc                                                                 <  2:69646/139‑1 (MQ=255)                                                                 tgtTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTGGACAATc                                                                 <  1:1418593/139‑1 (MQ=255)                                                                            tCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTGGACAATCAGCTTACCGcc                                                      <  2:928638/139‑1 (MQ=255)                                                                            tCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTGGACAATCAGCTTACCGcc                                                      <  1:1267316/139‑1 (MQ=255)                                                                             cGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCattat                                                                                                          >  1:808732/1‑86 (MQ=255)                                                                             cGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCattat                                                                                                          <  2:808732/86‑1 (MQ=255)                                                                             cGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTGGACAATCAGCTTACCGCCa                                                     >  1:1113870/1‑139 (MQ=255)                                                                             cGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTGGACAATCAGCTTACCGCCa                                                     >  2:468819/1‑139 (MQ=255)                                                                               tGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTGGACAATCAGCTTACCGCCAGt                                                   >  2:256705/1‑139 (MQ=255)                                                                                 gAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTGGACAATCAGCTTACCGCCAGTAt                                                 <  1:428965/139‑1 (MQ=255)                                                                                    gCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTGGACAATCAGCTTACCGCCAGTATCaa                                              <  1:256705/139‑1 (MQ=255)                                                                                    gCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTGGACAATCAGCTTACCGCCAGTATCaa                                              <  1:662993/139‑1 (MQ=255)                                                                                        ttAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGt                                                                                                >  2:1100196/1‑85 (MQ=255)                                                                                        ttAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGt                                                                                                <  1:1100196/85‑1 (MQ=255)                                                                                                ccgccgGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTAt                                                                                           >  1:1426575/1‑82 (MQ=255)                                                                                                ccgccgGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTAt                                                                                           <  2:1426575/82‑1 (MQ=255)                                                                                                  gccgGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTGGACAATCAGCTTACCGCCAGTATCAATGGTAGTATTTTTc                                >  1:46249/1‑139 (MQ=255)                                                                                                      gATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTGGACAATCAGCTTACCGCCAGTATCAATGGTAGTATTTTTCGCCg                            <  1:468819/139‑1 (MQ=255)                                                                                                                      gCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTgg                                                                                                 <  2:576193/54‑1 (MQ=255)                                                                                                                      gCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTgg                                                                                                 >  1:576193/1‑54 (MQ=255)                                                                                                                       cTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTGGACAATCAGCTTACCGCCAGTATCAATGGTAGTATTTTTCGCCg                            >  2:1361357/1‑122 (MQ=255)                                                                                                                       cTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTGGACAATCAGCTTACCGCCAGTATCAATGGTAGTATTTTTCGCCg                            <  1:1361357/122‑1 (MQ=255)                                                                                                                                accaccGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTGGACAATCAGCTTACCGCCAGTATCAATGGTAGTATTTTTCGCCGAGGTTTTAGCCACTACCGTCAGTTCg  >  1:893708/1‑139 (MQ=255)                                                                                                                                       |                                                                                                                                    CATTTTTAGCTTCTGAATTCCTGATGTAGAAAGCATCACCATAACTGTTGGTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGTCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTGGACAATCAGCTTACCGCCAGTATCAATGGTAGTATTTTTCGCCGAGGTTTTAGCCACTACCGTCAGTTCG  >  NC_000913/2780998‑2781263

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑